Abstract
Strongly hydrophobic integral membrane proteins are difficult to purify and characterise using conventional biochemical techniques. Eight years ago my co-workers and I initiated a research programme using molecular biological techniques to help elucidate the structure and function of important proteins in the plasma membrane of plant cells. We first turned out attention to the H+-ATPase, since this enzyme is easy to assay and is one of the more abundant transport proteins in this membrane (Surrowy and Sussman, 1987).
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References
De Witt, N.D., Harper, J.F., and Sussman, M.R., 1991. Evidence for plasma membrane proton pump in phloem cells of higher plants. The Plant Journal, 1, 121–128.
Harper, J.F., Manney, L., de Witt, N.D., Yoo, M.H., and Sussman, M.R., 1990. The Arabidopsis thaliana plasma membrane H+-ATPase multigene family: genomic sequence and expression of a third isoform. Journal of Biological Chemistry, 265, 13601–13608.
Harper, J.F., Surowy, T.K., and Sussman, M.R., 1989. Molecular cloning and sequence of cDNA encoding the plasma membrane proton pump (H+-ATPase) of The Arabidopsis thaliana. Proceedings of the National Academy of Sciences, USA, 86, 1234–1238.
Harper, J.F., Schaller, G.E., Sussman, M.R., Putnam-Evans, C., Charbonneau, H., and Harmon, A.C., 1991. A calcium-dependent protein kinase with a regulatory domain that is similar to calmodulin. Science, 252; 951–954.
Janoshazi, A., Seifter, J.L., and Solomon, A.K., 1989. Interactions between anion exchange and other membrane proteins in rabbit kidney medullary collecting duct cells. Journal of Membrane Biology, 112, 39–49.
Janoshazi, A., and Solomon, A.K., 1989. Interaction among anion, cation and glucose transport proteins in the human red cell. Journal of Membrane Biology, 112, 25–37.
Katz, D., 1989; Studies on the plasma membrane H+-ATPase of oat roots - preparation and assay, cytological localisation and sulfhydryl chemistry. Ph.D. thesis, University of Wisconsin, Madison, WI.
Laimins, L.A., Rhonds, D.B., and Epstein, W., 1981. Osmotic control of kdp operon expression in Escherichia coli. Proceedings of the National Academy of Sciences, USA, 78, 464–468.
Pardo, J.M. and Serrano, R., 1989. Structure of plasma membrane H+-ATPase gene from the plant Arabidopsis thaliana. Journal of Biological Chemistry, 264, 8557–8562.
Schaller, G.E., Harmon, A.C., and Sussman, M., 1992. Characterization of calcium-and lipid-dependent protein kinase associated with the plasma membrane of oat. Biochemistry, in press.
Schaller, G.E., and Sussman, M.R., 1987. Kinase-mediated phosphorylation of the oat plasma membrane H+-ATPase. In Plant Membranes: Structure, Function and Biogenesis. UCLA Symposium on Molecular and Cellular Biology, New Series, Volume 63. Eds C. Leaver and H Sze.
Schaller, G.E. and Sussman, M.R., 1988. Phosphorylation of the plasma membrane H+-ATPase of oat roots by a calcium-stimulated protein kinase. Planta, 173, 509–518.
Schaller, G.E. and Sussman, M.R., 1988. Isolation and sequence of tryptic peptides from the oat plasma membrane H+-ATPase. Plant Physiology, 86, 512–516.
Surowy, T.K. and Sussman, M.R., 1987. Molecular cloning of the plant plasma membrane H+-ATPase. Plant and Soil, 99, 185–196.
Surowy, T.K., and Sussman, M.R., 1987. Immunological cross-reactivity and inhibitor sensitivities of the plasma membrane H+-ATPase from plants and fungi. Biochemica et Biophyica Acta, 848, 24–34.
Sussman, M.R. and Harper, J.F., 1989. Molecular biology of the plasma membrane of higher plants. The Plant Cell, 1, 953–960.
Sussman, M.R. and Sorowy, T.K., 1987. Physiology and molecular biology of membrane ATPases. In Oxford Surveys of Plant Molecular and Cellular Biology, Volume 4. pp 47–71. Ed B.J. Miflin. Oxford University Press, Oxford. (Review)
Vara, F., and Serrano, R., 1982. Partial purification and properties of the proton translocating ATPase of plant plasma membranes. Journal of Biological Chemistry, 252, 12826–12830.
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© 1992 Springer Science+Business Media New York
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Sussman, M.R. (1992). A Plethora of Plant Plasmalemma Proton Pumps. In: Cooke, D.T., Clarkson, D.T. (eds) Transport and Receptor Proteins of Plant Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3442-6_1
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DOI: https://doi.org/10.1007/978-1-4615-3442-6_1
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