Abstract
Tissue blood flow measurements using the H2-clearance technique with implanted polarographic microelectrodes was described by Aukland et al. in 1964 (1). The H2-clearance technique has important advantages over other well-established methods such as radioactively labeled microspheres or 14C-iodoantipyrine quantitative autoradiography. Its primary benefits are the capacity for repeated regional cerebral blood flow (rCBF) measurements in the same animal and the avoidance of radioactive tracer material. However, CBF values obtained by the H2-clearance technique frequently differ from those obtained by radioactive tracer methods. Rat CBF values measured by iodoantipyrine (2) were significantly higher than measurements obtained by H2-clearance (3). Chronic implantation of electrodes leads to higher CBF values (4). These concerns were addressed recently by Tomida et al. who reported low rCBF values measured by H2-clearance shortly after electrode insertion in gerbils (5). CBF progressively increased by 24 hours, at which time the H2-clearance and the iodoantipyrine measurements correlated well. “Recovery” of rCBF was attributed to gradual resolution of spreading depression after the local trauma of electrode insertion (5).
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Uhl, M.W., Kochanek, P.M., Schiding, J.K., Melick, J.A., Nemoto, E.M. (1992). The Regional Cerebral Blood Flow Response to Cortical Microelectrode Insertion is Neutrophil Dependent. In: Erdmann, W., Bruley, D.F. (eds) Oxygen Transport to Tissue XIV. Advances in Experimental Medicine and Biology, vol 317. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3428-0_84
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DOI: https://doi.org/10.1007/978-1-4615-3428-0_84
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