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The Simultaneous Measurement of the Redox State of Cytochrome Oxidase in Heart and Brain of Rat in Vivo by NIR

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Oxygen Transport to Tissue XIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 316))

Abstract

Cytochrome oxidase(Cyt.ox.), which contains heme and copper, is a terminal enzyme of respiratory chain and transfers electrons to molecular oxygen (Erecinska & Wilson 1978). For this reason, monitoring oxidation-reduction state of Cyt.ox. provides the information about the pathologic state of tissues caused by impaired oxygen supply. The Cyt.ox., hemoglobin(Hb) and myoglobin(Mb) have a broad absorption band in near infrared(NIR) region and the light in this region easily penetrates biological tissues. Thus NIR spectrophotometry can measure noninvasively the redox state of Cyt.ox. and oxygenation of Hb and Mb through skin and bone. In this paper, to obtain the mutual relationship between the oxygenation state of cardiac and brain tissues, we examined the redox behavior of copper in Cyt.ox. with anesthetized rat.

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References

  • Erecinska, M., Wilson, D.F. (1978) “Cytochrome C oxidase; A synopsis.” Arch.Biochem.Biophys. 188:1

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  • Hazeki, O., and Tamura, M., (1989) “Near infrared quadruple wavelength spectrophotometry of rat head” Adv.Exp.Med.Biol. 248:63

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Author information

Authors and Affiliations

  1. Biophysics Division, Research Institute of Applied Electricity, Hokkaido University, Sapporo, 060, Japan

    Yasuyuki Kakihana, Kinji Ito & Mamoru Tamura

Authors
  1. Yasuyuki Kakihana
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  2. Kinji Ito
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  3. Mamoru Tamura
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Editor information

Editors and Affiliations

  1. Northwestern University, Evanston, Illinois, USA

    Thomas K. Goldstick

  2. University of Kuwait, Sulaibikhat, Kuwait

    Michael McCabe

  3. Griffith University, Brisbane, Queensland, Australia

    David J. Maguire

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© 1992 Springer Science+Business Media New York

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Kakihana, Y., Ito, K., Tamura, M. (1992). The Simultaneous Measurement of the Redox State of Cytochrome Oxidase in Heart and Brain of Rat in Vivo by NIR. In: Goldstick, T.K., McCabe, M., Maguire, D.J. (eds) Oxygen Transport to Tissue XIII. Advances in Experimental Medicine and Biology, vol 316. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3404-4_14

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  • DOI: https://doi.org/10.1007/978-1-4615-3404-4_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6504-4

  • Online ISBN: 978-1-4615-3404-4

  • eBook Packages: Springer Book Archive

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