Abstract
Continuous recording of changes in local pO2 during and after brain infarction in surviving animals which can be followed for months or years, may provide interesting information concerning pathophysiology and treatment of stroke and thrombosis. We performed such measurements before, during and till 4 weeks after photochemical induction of a cerebrocortical infarction in three rabbits.
Rose bengal - a photosensitive dye which sticks to endothelial cells and gives rise to endothelial damage and thrombosis when illuminated - was injected intravenously. After injection, a circular area (diameter 3 mm) of the brain cortex was illuminated using an optic fiber conducting light from a halogen lamp, whether or not filtered by heat and colour filters.
In order to enable pO2 measurement in and near the infarct zone, we constructed a transparent plastic frame in which pO2 electrodes were fixed beneath and 1 mm besides a shaft permitting mounting of the optic fiber. A black adhesive ring (inner diameter 3 mm) was attached to the bottom of the frame providing a perfectly demarcated illumination area. After fixation the electrodes were calibrated and the frame was implanted in the rabbit’s skull.
Ten days later an infarction was induced; pO2 was monitored continuously before, during and till 4 hours after this induction. Furthermore, pO2 was recorded 24 hours, 48 hours, 5 days, 14 days and 4 weeks after infarction.
Parameters describing the time course of pO2 were determined. In the illuminated area pO2 decreased after a certain latency time to reach a very low level, probably zero level, where it remained for at least 24 hours. Gradually, recovery was observed during the following days, and four weeks after infarction both level and pattern of electrode current appeared to be normal again. In the border zone pO2 decreased but did not reach zero level. Recovery was observed earlier than in the illuminated area.
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References
Busto, R., Dietrich, W. D., Globus, M. Y.-T., Valdes, I., Scheinberg, P., and Ginsberg, M. D., 1987, Small differences in intraischemic brain temperature critically determine the extent of ischemic neuronal injury, J. of Cerebral Blood Flow and Metabolism, 7: 729–738.
Colin, F., Manil, J., and Bourgain, R. H., 1981, The effect of acute anoxia on the cortical somatosensory evoked potential in the rabbit, Neurological Research, 3(4): 393–407.
De Ryck, M., Van Reempst, J., Borgers, M., Wauquier, A., and Janssen, P. A. J., 1989, Photochemical stroke model: flunarizine prevents sensorimotor deficits after neocortical infarcts in rats, Stroke, 20(10): 1383–1390.
Dietrich, W. D., Ginsberg, M. D., Busto, R., and Watson, B. D., 1986, Photochemically induced cortical infarction in rat. 1. Time course of hemodynamic consequences. 2. Acute and subacute alterations in local glucose utilization, J. of Cerebral Blood Flow and Metabolism, 6: 184–202.
Ginsberg, M. D., Castella, Y., Dietrich, W. D., Watson, B. D., and Busto, R, 1989, Acute thrombotic infarction suppresses metabolic activation of ipsilateral somatosensory cortex: evidence for functional diaschisis, J. of Cerebral Blood Flow and Metabolism, 9: 329–341.
Graham, D. I., 1988, Focal cerebral infarction, Journal of Cerebral Blood Flow and Metabolism, 8: 769–773.
Grome, J. J., Gojowczyk, G., Hofmann, W., and Graham, D. I., 1988, Quantitation of photochemically induced focal cerebral ischemia in the rat, J. of Cerebral Blood Flow and Metabolism, 8: 89–95.
Royster, A. J., Nanda, S. K., Hatchell, D. L., Tiedeman, J. S., Dutton, J. J., and Hatchell, M. C., Photochemical initiation of thrombosis Fluorescein angiographic, histologic and ultrastructural alterations in the choroid, retinal pigment epithelium and retina, Arch. Ophtalmol., 106: 1608–1614.
Van Reempst, J., Van Deuren, B., Van de Ven, M., Cornelissen, F., and Borgers, M., 1987, Flunarazine reduces cerebral infarct size after photochemically induced thrombosis in spontaneously hypertensive rats, Stroke, 18(6): 1113–1119.
van Rossem, K., Vermariën, H., Jacqueloot, J., and Bourgain, R., 1989, Continuous sensing of oxygen tension in rabbit brain cortical tissue during and after photochemically induced infarction, Proceedings V Mediterranean Conf Med. Biol. Eng., Patras, 190–191.
Van Waeyenberge, M., Vermariën, H., De Backer, H., Manil, J., and Bourgain, R. H., 1985, Discriminant parameters representing cerebral cortical function during anoxic anoxia investigations, In: “Advances in Experimental Medicine and Biology”, F. Kreuzer, S. M. Cain, Z. Turek and T. K. Goldstick, eds., vol. 191: Oxygen Transport to Tissue VII, Plenum, New York, 149–161.
Watson, B. D., Dietrich, W. D., Busto, R., Wachtel, M. S., and Ginsberg, M. D., 1985, Induction of reproducible brain infarction by photochemically initiated thrombosis, Ann. Neurol., 17: 497–504.
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© 1992 Springer Science+Business Media New York
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van Rossem, K., Vermariën, H., Decuyper, K., Bourgain, R. (1992). Photothrombosis in Rabbit Brain Cortex: Follow up by Continuous pO2 Measurement. In: Goldstick, T.K., McCabe, M., Maguire, D.J. (eds) Oxygen Transport to Tissue XIII. Advances in Experimental Medicine and Biology, vol 316. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3404-4_11
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DOI: https://doi.org/10.1007/978-1-4615-3404-4_11
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