Abstract
Localization of basic fibroblast growth factor (bFGF) in a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma of rat was determined by two immunological techniques using a specific monoclonal antibody against bFGF. Concentration of bFGF in cell extract was measured by sandwich radioimmunoassay (RIA) with heparin-Sepharose and 125I-labeled monoclonal antibody. bFGF concentration in the extract of AT-3 cells increased with increasing concentration of NaCl in extraction buffer. Localization of bFGF in AT-3 cells was determined by counting radioactivity of 125I-labeled monoclonal antibody binding to AT-3 cells before or after increasing permeability of the cells. The binding increased significantly by this treatment, indicating that bFGF within the cells was detected.
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Abbreviations
- FGF:
-
fibroblast growth factor
- bFGF:
-
basic fibroblast growth factor
- aFGF:
-
acidic fibrolast growth factor
- CHAPS:
-
3-[(3-chol-amidopropyl) dimenthylammonio]-1-propane sulfonate:
- PBS:
-
phosphate buffered saline
- BSA:
-
bovine serum albumin
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Nishikawa, K., Yoshitake, Y., Minemura, M., Yamada, K., Matuo, Y. (1992). Localization of Basic Fibroblast Growth Factor (bFGF) in a Metastatic Cell Line (AT-3) Established from the Dunning Prostatic Carcinoma of Rat: Application of a Specific Monoclonal Antibody. In: Karr, J.P., Yamanaka, H. (eds) Prostate Cancer and Bone Metastasis. Advances in Experimental Medicine and Biology, vol 324. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3398-6_13
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DOI: https://doi.org/10.1007/978-1-4615-3398-6_13
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