CD28 Receptor Crosslinking Induces Tyrosine Phosphorylation of PLCγ1

  • Jeffrey A. Ledbetter
  • Peter S. Linsley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 323)


The CD28 receptor has been widely recognized for its ability to regulate T cell responses to T cell receptor (TCR) stimulation (see 1 for review). CD28 crosslinking upregulates cytokine mRNA expression and secretion 2,3 when CD28 is co-ligated with the CD3/TCR and can directly induce IL2 Receptor ∝ chain mRNA expression in resting T cells 4 in the absence of other signals. Despite the importance of the CD28 receptor, the mechanisms of CD28 signal transduction are not yet known. Here we provide evidence that phospholipase C (PLC) γ1 is phosphorylated on tyrosine after CD28 ligation on primed T cells. In addition, the CD28-mediated induction of IL2 mRNA is inhibited by the tyrosine kinase inhibition herbimycin A, suggesting that the IL2 mRNA response may be partly caused by PLC γ1 activation.


Tyrosine Phosphorylation Chinese Hamster Ovary Cell CD28 Receptor CD28 Ligation Induce Tyrosine Phosphorylation 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Jeffrey A. Ledbetter
    • 1
  • Peter S. Linsley
    • 1
  1. 1.Bristol-Myers Squibb Pharmaceutical Research InstituteSeattleUSA

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