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Dissection of the Hb(64–76) Determinant Reveals That the T Cell Receptor May Have the Capacity to Differentially Signal

  • Brian D. Evavold
  • Paul M. Allen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 323)

Abstract

The T cell inducing determinant Hb(64-76) was initially identified in studies examining the processing and presentation of the self antigen hemoglobin1. Murine hemoglobin exists in two allelic forms, Hbbs and Hbbd. Immunization of H-2k mice expressing the Hbbs allele, e.g. CE/J, with purified hemoglobin from Hbbd mice, e.g. CBA/J, resulted in a strong T cell response. This allelic response is entirely directed against a single determinant composed of residues 64-76 of the Hbbdminor chain. Three of the 13 amino acid differences between the s and d allelic forms of hemoglobin are found in this region.

Keywords

Critical Residue Allelic Form Contact Residue Wild Type Peptide Conservative Amino Acid Substitution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    R.G. Lorenz and P.M. Allen, Direct evidence for functional self protein/la-molecule complexes in vivo, Proc. Natl. Acad. Sci. USA 85:5220 (1988).Google Scholar
  2. 2.
    R.G. Lorenz and P.M. Allen, Thymic cortical epithelial cells lack full capacity for antigen presentation, Nature 340:557 (1989).Google Scholar
  3. 3.
    R.G. Lorenz and P.M. Allen, Thymic cortical epithelial cells can present self antigens in vivo, Nature 337:560 (1989).Google Scholar
  4. 4.
    B.D. Evavold, S.G. Williams, B.L. Hsu, S. Buus and P.M. Allen, Complete dissection of the Hb(64-76) determinant using Th1, Th2 clones, and T cell hybridomas, J. Immunol. 148:347 (1992).Google Scholar
  5. 5.
    B.S. Fox, C. Chen, E. Frage, C.A. French, B. Singh and R.H. Schwartz, Functionally distinct agretopic and epitopic sites. Analysis of the dominant T cell determinant of moth and pigeon cytochrome c with the use of synthetic peptide antigens, J Immunol. 139:1578 (1987).Google Scholar
  6. 6.
    P.M. Allen, G.R. Matsueda, R.I. Evans, Jr. J.B. Dunbar, G.R. Marshall and E.R. Unanue, Dissection of a T cell antigenic epitope: Identification of the T cell and Ia contact residues, Nature 327:713 (1987).Google Scholar
  7. 7.
    R.G. Lorenz, A.N. Tyler and P.M. Allen, Reconstruction of the immunogenic peptide RNase(43-56) by identification and transfer of the critical residues into an unrelated peptide backbone, J. Exp. Med. 170:203 (1989).Google Scholar
  8. 8.
    B.D. Evavold and P.M. Allen, Separation of IL-4 production from Th cell proliferation by an altered T cell receptor ligand, Science 252:1308 (1991).Google Scholar
  9. 9.
    F. Letourneur and R.D. Klausner, Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 ε, Science 255:79 (1992).Google Scholar
  10. 10.
    A.-M.K. Wegener, F. Letourneur, A. Hoeveler, T. Brocker, F. Luton and F. Malissen, The T cell receptor/CD3 complex is composed of at least two autonomous transduction modules, Cell 68:83 (1992).Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Brian D. Evavold
    • 1
  • Paul M. Allen
    • 1
  1. 1.Department of PathologyWashington University School of MedicineSt. LouisUSA

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