T Cell Receptor Structure and Function: Analysis by Expression of Portions of Isolated Subunits

  • Isaac Engel
  • Francois Letourneur
  • John T. B. Houston
  • Tom H. M. Ottenhoff
  • Richard D. Klausner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 323)


The T cell antigen receptor (TCR) is a multi-component cell surface complex composed of the products of at least six genes (1,2). Specific recognition of antigen/MHC is mediated by two chains of the TCR complex (generally ∝ and β) that are expressed as disulfide linked heterodimers and display a high degree of clonotypic diversity. Associated with this specialized heterodimer are the invariant dimers CD3 ε-δ and CD3 ε-γ and a disulfide-linked dimer formed by members of the ζ gene family (typically a ζ-ζ homodimer). The invariant chains of the TCR have been shown to be necessary for efficient assembly and transport of the receptor to the cell surface (3), and are also thought to be essential for signal transduction.


Cytoplasmic Tail Invariant Chain Cell Antigen Receptor Heterodimer Formation Chimeric Construct 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    H.C. Clevers, B. Alarcon, T.E. Wileman, and C. Terhorst, The T-cell receptor/CD3 complex: A dynamic protein ensemble, Annu. Rev. Immunol. 6:629 (1988).Google Scholar
  2. (2).
    R.D. Klausner, J. Lippincott-Schwartz, and I.S. Bonifacino, The T-cell antigen receptor: Insights into organelle biology, Annu. Rev. Cell. Biol. 6:403 (1990).Google Scholar
  3. (3).
    R.D. Klausner, I. Lippincott-Schwartz, and I.S. Bonifacino, Architiectural editing: Regulating the surface expression of the multicomponent T-cell antigen receptor, Current Topics in Membranes and Transport 36:31 (1990).Google Scholar
  4. (4).
    R.D. Klausner, and L.E. Samelson, T cell antigen receptor activation pathways: The tyrosine kinase connection, Cell 64:875 (1991).Google Scholar
  5. (5).
    D.I. Park, H.W. Rho, and S.O. Rhee, CD3 stimulation causes phosphorylation of phospholipase C-γ1 on serine and tyrosine residues in a human T-cell line, Proc. Natl. Acad. Sci. USA 88:5453 (1991).Google Scholar
  6. (6).
    A. Weiss, O. Koretsky, R. Schatzman, and T. Kadlecek, Functional activation of the T cell antigen receptor induces tyrosine phosphorylation of phospholipase C-γ1, Proc. Natl. Acad. Sci. USA 88:5484 (1991).Google Scholar
  7. (7).
    L.E. Samelson, M.D. Patel, A.M. Weissman, I.B. Harford, and R.D. Klausner, Antigen activation of murine T cells induces tyrosine phosphorylation of a polypeptide associated with the T cell antigen receptor, Cell 46:1083 (1986).Google Scholar
  8. (8).
    J.J. Sussman et al., Failure to synthesize the T cell CD3-ζ chain: structure and function of a partial T cell receptor complex, Cell 52:85 (1988).Google Scholar
  9. (9).
    S.I. Frank et al., Structural mutations of the T cell receptor ζ chain and its role in T cell activation, Science 249:174 (1990).Google Scholar
  10. (10).
    B.A. Irving and A. Weiss, The cytoplasmic domain of the T cell receptor ζ chain is sufficient to couple to receptor-associated signal transduction pathways, Cell 64:891 (1991).Google Scholar
  11. (11).
    C. Romeo and B. Seed, Cellular immunity to mv activated by CD4 fused to T cell or Fc receptor polypeptides, Cell 64:1037 (1991).Google Scholar
  12. (12).
    F. Letourneur, and R.D. Klausner, T-cell and basophil activation through the cytoplasmic tail of T-cell-receptor ε family proteins, Proc. Natl. Acad. Sci. USA 88:8905 (1991).Google Scholar
  13. (13).
    A.-M.K. Wegener et al., The T cell receptor/CD3 complex is composed of at least two autonomous transduction modules, Cell 68:83 (1992).Google Scholar
  14. (14).
    F. Letourneur, and R.D. Klausner, Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 ε, Science 255:79 (1992).Google Scholar
  15. (15).
    M. Reth, Antigen receptor tail clue, Nature 338,383 (1989).Google Scholar
  16. (16).
    A.Y. Lin et al., Expression of T cell antigen receptor heterodimers in a lipid-linked form, Science 249:677 (1990).Google Scholar
  17. (17).
    A.E. Slanetz and A.L.M. Bothwell, Heterodimeric, disulfide-linked ∝/β T cell receptors in solution, Eur. J. Immunol. 21:179 (1991).Google Scholar
  18. (18).
    C.K. Suzuki, and R.D. Klausner, unpublished observations.Google Scholar
  19. (19).
    T.M. Rutledge, P. Cosson, N. Manolios, I.S. Bonifacino, and R.D. Klausner, in preparation.Google Scholar
  20. (20).
    S.M. Hedrick et al., The fine specificity of antigen and Ia determinant recognition of T cell hybridoma clones specific for pigeon cytochrome c, Cell 30:141 (1982).Google Scholar
  21. (21).
    I. Engel and R.D. Klausner, unpublished observations.Google Scholar
  22. (22).
    I. Engel, T.H.M. Ottenhoff, and R.D. Klausner, High-efficiency expression and solubilization of functional T cell antigen receptor heterodimers, Science 256:1318.Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Isaac Engel
    • 1
  • Francois Letourneur
    • 1
  • John T. B. Houston
    • 1
  • Tom H. M. Ottenhoff
    • 1
  • Richard D. Klausner
    • 1
  1. 1.Cell Biology and Metabolism BranchNational Institute of Child Health and Human Development, National Institutes of HealthBethesdaUSA

Personalised recommendations