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Abstract

The application of immunochemical methods in veterinary drug residues is discussed. These methods concern (i) immunochemical determinations such as ELISAs for rapid screening purposes and (ii) immunoaffinity clean-up techniques for crude samples or sample extracts prior to a physicochemical determination. Examples of these methods are given. Special attention is paid to the detection and/or determination of chloramphenicol residues by these techniques. This concerns a rapid detection method using a streptavidin-biotin ELISA and an immunoaffinity clean-up, both off-line and by means of a fully automated HPLC system.

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References

  1. Haagsma, N., 1990, Sample preparation in drug residue analysis, in: “Proceedings of the EuroResidue Conference”, Noordwijkerhout, The Netherlands, May 1990, N. Haagsma, A. Ruiter and P.B. Czedik-Eysenberg, eds., State University, Utrecht, p. 40.

    Google Scholar 

  2. Smith, C.J., 1990, Evolution of the immunoassay. In: Development and application of immunoassay for food analysis. J.H. Rittenberg Ed., Elsevier Applied Science, London/New York, p. 3.

    Google Scholar 

  3. Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibodies of predefined specificity, Nature 256: 495.

    Article  PubMed  Google Scholar 

  4. Yalow, R.S., and Berson, S.A., 1959, Assay of plasma insulin in human subjects by immunological methods, Nature 184: 1648.

    Article  PubMed  CAS  Google Scholar 

  5. Ekins, R.P, 1960, The estimation of thyroxine in human plasma by an electrophoretic technique, Clin. Chim. Acta 5:453.

    Article  PubMed  CAS  Google Scholar 

  6. Engvall, E., and Perlmann, P., 1971, Enzyme-linked immunosorbent assay (ELISA), Quantitative assay of immunoglobulin G, Immunochemistry 8: 871.

    Article  PubMed  CAS  Google Scholar 

  7. Weemen, B.K. van, and Schuurs, A.H.W.M., 1971, Immunoassay using antigen-enzymeconjugates, FEBS Letters 15: 232.

    Article  PubMed  Google Scholar 

  8. Morris, B.A., and Clifford, M.N., 1985, “Immunoassays in food analysis”, Elsevier Applied Science publishers, London.

    Google Scholar 

  9. Benraad, Th.J., Stephany, R.W., Rosmalen, F.M.A., Hofman, J.A., Loeber, J.G., and Evers, L.H., 1981, The necessity of chromatographic purification prior to radio-immunoassay of diethylstilbestrol in the urine of cattle. Vet. O., 3:153.

    Article  CAS  Google Scholar 

  10. Rattenberger, E., Matzke, P., and Neudegger, J., 1985, Entwicklung eines Radioimmunotests zur Erfassung von Rückständen des ß-Rezeptorenblockers Carazolol in Blut und Ham von Schweinen, Arch. Lebensmit-telhyg., 36:85.

    CAS  Google Scholar 

  11. Arnold, D., Berg, D. vom, Boertz, A.K., Mallick, U., and Somogyi, A., 1984, Radioimmunologische Bestimmung von Chloramphenicol-Rückständen in Muskulatur, Milch und Eier, Arch. Lebensmittelhyg., 35:131.

    CAS  Google Scholar 

  12. Porstmann, T., Porstmann, B., and Seifert, R., 1983, Application of the peroxidase-antiperoxidase system as an universal reagent for the two-site binding enzyme immunoassay, Clin. Chim. Acta, 129:107.

    Article  PubMed  CAS  Google Scholar 

  13. Stanley, C.J., Johannsson, A., and Self, C.H., 1985, Enzyme amplification can enhance both the speed and the sensitivity of immunoassays, J. Immunol. Methods, 83:89.

    Article  PubMed  CAS  Google Scholar 

  14. Carr, R.I., Mansour, M., Sadi, D., James, H., and Jones, J.V., 1987, A substrate amplification system for enzyme-linked immunoassays. Demontration of its general applicability to ELISA systems for detecting antibodies and immune complexes, J. Immunol. Methods, 98:201.

    Article  PubMed  CAS  Google Scholar 

  15. Wilchek, M., and Bayer, E.A., 1988, The avidin-biotin complex in bioanalytical applications, Anal. Biochem., 171:1.

    Article  PubMed  CAS  Google Scholar 

  16. Guesdon, J., Ternynck, T., and Avrameas, S., 1979, The use of avidin-biotin interaction in immunoenzymatic techniques, J. Histochem. Cytochem., 27:1131.

    Article  PubMed  CAS  Google Scholar 

  17. Fleeker, J.R., and Lovett, L.J., 1985, Enzyme immunoassay for screening sulfamethazine in swine blood, J. Assoc. Off. Anal. Chem., 68:172.

    PubMed  CAS  Google Scholar 

  18. Singh, P., Ram, B.P., and Sharkvo, N., 1989, Enzyme immunoassay for screening of sulfamethazine in swine, J. Agric Food Chem., 37:109.

    Article  CAS  Google Scholar 

  19. Dixon-Holland, D.E., and Katz, S.E., 1988, Competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue, J. Assoc. Off. Anal. Chem., 71:1137.

    PubMed  CAS  Google Scholar 

  20. Dixon-Holland, D.E., and Katz, S.E., 1988, Direct competitive enzyme-linked immunosorbent assay for sulfamethazine residues in milk, J. Assoc. Off. Anal. Chem., 72:447.

    Google Scholar 

  21. Zwickl, C.M., Smith, H.W., and Bick, P.H., 1990, Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk, J. Agric. Food Chem., 38:1358.

    Article  Google Scholar 

  22. Kitagawa, T., Gotoh, Y., Uchiyama, K., Kohri, Y., Kinoue, T., Fujiwara, K., and Ohtani, W., 1988, Sensitive immunoassay of cephalexin residues in milk, hen tissues and eggs, J. Assoc. Off. Anal. Chem., 71:915.

    PubMed  CAS  Google Scholar 

  23. Märtlbauer, E. and Terplan, G., 1987, Ein enzymimmunologischer Nachweis von Chloramphenicol in Milch, Arch. Lebensmittelhyg., 38:3.

    Google Scholar 

  24. Water, C. van de, and Haagsma, N., 1990, A sensitive streptavidin-biotin enzyme-linked immunosorbent assay for rapid screening of residues of chloramphenicol in milk, Food Agr, Immunol., 2:11.

    Article  Google Scholar 

  25. Water, C. van de, and Haagsma, N., 1991, Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods, J.Chromatogr., 566:173.

    Article  PubMed  Google Scholar 

  26. Water, C. van de, Haagsma, N., Kooten, P.J.S. van, and Eden, W. van, 1987, An enzyme-linked immunosorbent assay for the determination of chloramphenicol using a monoclonal antibody, Z. Lebensm. Unters. Forsch., 185:202.

    Article  PubMed  Google Scholar 

  27. Water, C. van de, and Haagsma, N., 1990, A sensitive streptavidin-biotin enzyme-linked immunosoebent assay for rapid screening of residues of chloramphenicol in swine muscle tissues, J. Assoc. Off. Anal. Chem., 73:534.

    PubMed  Google Scholar 

  28. Mount, M.E., and Failla, D.L., 1987, Production of antibodies and development of enzyme immunoassays for determination of monensin in biological samples, J. Assoc. Off, Anal. Chem., 70:201.

    CAS  Google Scholar 

  29. Nouws, J.F.M., Beek, F., Aerts, M.M.L., Baakman, M., and Laurensen, J., 1987, Monitoring slaughtered animals for chloramphenicol residues by an immunoassay test kit (Quik-card), Arch. Lebensmittelhyg., 38:9.

    Google Scholar 

  30. Nouws, J.F.M., Laurensen, J., and Aerts, M.M.L., 1987, Monitoring of chloramphenicol residues in eggs by HPLC and an immunoassay (QuikCard), Arch. Lebensmittelhyg., 38:7.

    CAS  Google Scholar 

  31. Nouws, J.F.M., Laurensen, J., and Aerts, M.M.L., 1988, Monitoring milk for chloramphenicol residues by an immunoassay (Quik-card), Vet. O., 10:270.

    Article  CAS  Google Scholar 

  32. Haagsma, N., Schreuder, C., and Rensen, E.R.A., 1986. Rapid sample preparation method for the determination of chloramphenicol in swine muscle by high-performance liquid chromatography, J. Chromatogr., 363:353.

    Article  PubMed  CAS  Google Scholar 

  33. Cuatrecasas, P., Wilchek, M., and Anfinsen, C.B., 1968, Selective enzyme purification by affinity chromatography, Proc. Natl. Acad. Sci. U.S.A., 61:636.

    Article  PubMed  CAS  Google Scholar 

  34. Livingstone, D.M., 1974, Immunoaffinity chromatography of proteins, in: “Methods in Enzymology”, vol. XXIV, W.B. Jakoby and M. Wilchek, eds., p. 723.

    Google Scholar 

  35. Katz, S.E., and Brady, M.S., 1990, High-performance immunoaffinity chromatography for drug residue analysis, J. Assoc. Off. Anal. Chem., 73:557.

    PubMed  CAS  Google Scholar 

  36. Ginkel, L.A. van, 1991, Immunoaffinity chromatography, its applicability and limitations in multi-residue analysis of anabolizing and doping agents, J. Chromatogr., 564:363.

    Article  PubMed  Google Scholar 

  37. Dean, P.D.G., Johnson, W.S., and Middle, F.A., eds., 1985, “Affinity chromatography, a practical approach”, IRL Press Ltd., Oxford.

    Google Scholar 

  38. Bethell, G.S., Ayers, J.S., Hearn, M.T.W., and Hancock, W.S., 1981, Investigation of the activation of various insoluble polysaccharides with 1,1’carbonyldiimidazole and of the properties of the activated matrices, J. Chromatogr., 219:361.

    CAS  Google Scholar 

  39. Hearn, M.T.W., Harris, E.L., Bethell, G.S., Hancock, W.S., and Ayers, J.A., 1981, Application of 1,1’-carbonyldiimidazole-activated matrices for the purification of proteins. III. The use of 1,1’-carbonyldiimidazole-activated agaroses in the biospecific affinity chromatographic isolation of serum antibodies, J. Chromatogr., 218:509.

    Article  PubMed  CAS  Google Scholar 

  40. Prisyazhnoy, V.S., Fusek, M., and Alakhov, Y.B., 1988, Synthesis of high-capacity immunoaffinity sorbents with oriented immobilized immunoglobulins or their Fab’ fragments for isolation of proteins, J. Chromatogr. Biomed. Appl., 424:243.

    Article  CAS  Google Scholar 

  41. Matson, R.S., and Little, M.C., 1988, Strategy for the immobilization of monoclonal antibodies on solid-phase supports, J. Chromatogr., 458:67.

    Article  PubMed  CAS  Google Scholar 

  42. Scatchard, G., 1949, The attractions of proteins for small molecules and ions, Ann. N.Y. Acad. Sci., 51:660.

    Article  CAS  Google Scholar 

  43. Oss, C.J. van, Good, R.J., and Chaudhury, M.K., 1986, Nature of the antigen-antibody interaction. Primary and secondary bonds: optimal conditions for association and dissociation, J. Chromatogr. Biomed. Appl., 376:111.

    Article  Google Scholar 

  44. Ginkel, L.A. van, Blitterswijk, H. van, Zoontjes, P.W., Bosch, D. van den, and Stephany, R.W., 1988, Assay for trenbolone and its metabolite 17atrenbolone in bovine urine based on immunoaffinity chromatographic clean-up and off-line high-performance liquid chromatography - thin-layer chromatography, J. Chromatogr., 445:385.

    Article  PubMed  Google Scholar 

  45. Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, Blitterswijk, H. van, Zoontjes, P.W., Hooyschuur, R.C.M., and Zuyderdorp, J., 1989, Effective monitoring of residues of nortestosterone and its major metabolite in bovine urine and bile, J. Chromatogr., 489:95.

    Article  PubMed  Google Scholar 

  46. Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, Steinbuch, H.M., Zomer, G., Heeft, E. van de, and Jong, A.P.J.M. de, 1989, Multi-immunoaffinity chromatography: a simple and highly selective clean-up method for multi-anabolic residue analysis of meat, J. Chromatogr., 489:111.

    Article  PubMed  Google Scholar 

  47. Farjam, A., Jong, G.J. de, Frei, R.W., Brinkman, U.A. Th., Haasnoot, W., Hamers, A.R.M., Schilt, R. and Huf, F.A., 1988, Immunoaffinity pre-column for selective on-line sample pretreatment in high-performance liquid chromatography determination of 19-testosterone, J. Chromatogr., 452:419.

    Article  PubMed  CAS  Google Scholar 

  48. Haasnoot, W., Schilt, R., Hamers, A.R.M., Huf, F.A., Farjam, A., Frei, R.W., and Brinkman, U.A.Th., 1989, Determination of ß-19-nortestosterone and its metabolite α-19-nortestosterone in biological samples at the subparts per billion level by high-performance chromatography with online immunoaffinity sample pretreatment, J. Chromatogr., 489:157.

    Article  PubMed  CAS  Google Scholar 

  49. Bagnati, R., Castelli, M.G., Airoldi, L., Oriundi, M.P., Ubaldi, A., and Fanelli, R, 1990, Analysis of diethylstilbestrol, dienestrol and hexestrol in biological samples by immunoaffinity extraction and gas chromatography - negative-ion chemical ionization mass spectrometry, J. Chromatogr., 527:267.

    Article  PubMed  CAS  Google Scholar 

  50. Bagnati, R., and Fanelli, R., 1991, Determination of 19-nortestosterone, testosterone and trenbolone by gas chromatograpy - negative-ion mass spectrometry after formation of the pentafluorobenzylcarboxymethoximetrimethylsilyl derivatives, J. Chromatogr., 547:325.

    Article  PubMed  CAS  Google Scholar 

  51. Schilt, R., Haasnoot, W., Jonker, M.A., Hooyerink, H., and Paulussen, R.J.A., 1990, Determination of ß-agonistic drugs in feed, urine and tissue samples of cattle with immunoaffinity clean-up and GC-MS, in: “Proceedings of the EuroResidue Conference”, Noordwijkerhout, The Netherlands, May 1990, N. Haagsma, A. Ruiter and P.B. Czedik-Eysenberg, eds., State University, Utrecht, p. 320.

    Google Scholar 

  52. Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, and Fula, J., 1991, Multiresidue test for ß-agonists in a variety of matrices, in: “Proceedings of the International Symposium on Veal Calf Production”, Wageningen, The Netherlands, March 1990, J.H.M. Metz and C.M. Groenestein, eds., Pudoc, Wageningen, 192.

    Google Scholar 

  53. Haasnoot, W., Ploum, M.E., Paulussen, R.J.A., Schilt, R., and Huf, F., 1990, Rapid determination of clenbuterol residues in urine by high-performance liquid chromatography with on-line automated sample processing using immunoaffinity chromatography, J. Chromatogr., 519:323.

    Article  PubMed  CAS  Google Scholar 

  54. Water, C. van de, and Haagsma, N., 1987, Determination of chloramphenicol in swine muscle tissue using a monoclonal antibody-mediated clean-up procedure, J. Chromatogr., 411:415.

    Article  PubMed  Google Scholar 

  55. Water, C. van de, Tebbal, D., and Haagsma, N., 1989, Monoclonal antibody-mediated clean-up procedure for the high-performance liquid chromatographic analysis of chloramphenicol in milk and eggs, J. Chromatogr., 478:205.

    PubMed  Google Scholar 

  56. Moretti, V.M., Water, C. van de, and Haagsma, N., 1991, Automated determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up and column switching technique, in preparation.

    Google Scholar 

  57. Clovis, Y.D., 1987, Matrix evaluation for preparative high-performance affinity chromatography, J. Chromatogr., 407:179.

    Article  Google Scholar 

  58. Josic, D., Hofmann, W., Habermann, R., Becker, A., and Reutter, W., 1987, High-performance liquid affinity chromatography of liver plasma membrane proteins, J. Chromatogr., 397:39.

    Article  PubMed  CAS  Google Scholar 

  59. Phillips„ T.M., and Frantz, S.C., 1988, Isolation of specific lymphocyte receptors by high-performance immunoaffinity chromatography, J. Chromatogr., 444:13.

    Article  PubMed  Google Scholar 

  60. Nilsson, B., 1983, Extraction and quantitation of cortisol by use of high-performance liquid chromatography, J. Chromatog. Biomed. Appl., 276:413.

    Article  CAS  Google Scholar 

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Haagsma, N., van de Water, C. (1992). Immunochemical Methods in the Analysis of Veterinary Drug Residues. In: Agarwal, V.K. (eds) Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3356-6_7

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  • DOI: https://doi.org/10.1007/978-1-4615-3356-6_7

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