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Increased Calcium Release from Sarcoplasmic Reticulum Stimulated by Inositol Trisphosphate in Spontaneously Hypertensive Rat Heart Cells

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Book cover Cellular Function and Metabolism

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 9))

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Abstract

It is known that inositol (1, 4, 5)-trisphosphate (IP3) stimulates Ca2+ release from sarcoplasmic reticulum (SR) in several tissues, but in cardiac myocytes this phenomenon has not been confirmed. The purpose of the present study was to confirm the effect of (1, 4, 5)-IP3 on Ca2+ release from SR in cardiac myocytes. The effect of IP3 on Ca2+ release from SR in hypertrophic cardiac cells was also determined.

We examined the effects of IP3 on Ca2+ release from cardiac myocyte SR by the bigital-image method in a single cell. We also determined the effect of IP3 on calcium release from isolated SR. SR was prepared from spontaneous hypertensive rat hearts and Wistar kyoto rat hearts. The SR was prelabeled with 45Ca2+, and then incubated with the indicated concentrations of IP3 for 1 min at 37° C. In cardiac myocytes treated with saponin, Ca2+ release stimulated by 10 μM (1, 4, 5)-IP3 was detected by fura-2. In 45Ca2+ prelabeled SR, the maximal Ca2+ release was achieved at 10 μM IP3 incubated for 1 min. The release of Ca2+ was higher in SR of SHR than in the SR of WKY. IP3 stimulates Ca2+ release from cardiac SR, and this release is greater in SHR than in WKY. However, it is uncertain whether this phenomenon plays a role in cardiac hypertrophy. (Mol Cell Biochem 119: 51–57, 1993)

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References

  1. Berridge MJ: Inositol trisphosphate and diacylglycerol as second messengers. Biochem J 220: 345–360, 1984

    PubMed  CAS  Google Scholar 

  2. Nisizuka Y: Phospholipid degradation and signal translation for protein phosphorylation. Trend Biochem Sci 8: 13–16, 1983

    Article  Google Scholar 

  3. Williamson JR, Cooper RH, Joseph SK, Thomas AP: Inositol trisphosphate and diacylglycerol as intracellular second messengers in liver. Am J Phys 248: C203–216, 1985

    CAS  Google Scholar 

  4. Enouf J, Giraud F, Bredoux R, Bourdeau N, Toledano SL: Possible role of cAMP-dependent phosphorylation in the calcium release mediated by inositol 1, 4, 5-trisphosphate in human platelet membrane vesicles. Biochem Biophys Acta 928: 76–82, 1987

    Article  PubMed  CAS  Google Scholar 

  5. Irvine RF, Letcher AJ, Heslop JP, Berridge MJ: The inositol tris/tetrakisphosphate pathway-demonstration of Ins(1, 4, 5)P3 3-kinase activity in animal tissues. Nature 320: 631–634, 1986

    Article  PubMed  CAS  Google Scholar 

  6. Kawaguchi H, Iizuka K, Takahashi H, Yasuda H: Inositol trisphosphate kinase activity in hypertrophied rat heart. Biochem Med Metabol Biol 44: 42–50, 1990

    Article  CAS  Google Scholar 

  7. Batty IR, Nahorski SR, Irvine RF: Rapid formation of inositol 1, 3, 4, 5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices. Biochem J 232: 211–215, 1985

    PubMed  CAS  Google Scholar 

  8. Irvine RF, Moor RM: Micro-injection of inositol 1, 3, 4, 5-tetrakisphosphate activities sea urchin eggs by a mechanism dependent on external Ca2+. Biochem J 240: 917–920, 1986

    PubMed  CAS  Google Scholar 

  9. Kawaguchi H, Shoki H, Sano H, Kudo T, Sawa F, Okamoto H, Sakata Y, Yasuda H: Phospholipid metabolism in cardiomyopathic hamster heart cells 69: 1015–1021, 1991

    CAS  Google Scholar 

  10. Shoki M, Kawaguchi H, Okamoto H, Sano H, Sawa H, Kudo T, Hirao H, Sakata Y, Yasuda H: Phosphatidylinositol and inositol-phosphatide metabolism in hypertrophied rat heart. Jpn Circ J 56: 142–147, 1992

    Article  PubMed  CAS  Google Scholar 

  11. Kawaguchi H, Shoki M, Sano H, Kudo T, Sawa H, Mochizuki N, Okamoto H, Endo Y, Kitabatake A: Polyphosphoinositide metabolism in hypertrophic rat heart. J Mol Cell Cardiol, in press

    Google Scholar 

  12. Movsesian MA, Thomas AP, Selak M, Williamson JR: Inosi tol trisphosphate does not release Ca2+ from permeabilized cardiac myocytes and sarcoplasmic reticulum. FEBS Lett 185: 328–332, 1985

    Article  PubMed  CAS  Google Scholar 

  13. Nosek TM, Williams MF, Zeigler ST: Inositol trisphosphate enhances calcium release in skinned cardiac and skeletal muscle. Am J Physiol 250: C807–811, 1986

    PubMed  CAS  Google Scholar 

  14. Fujii S, Kawaguchi H, Okamoto H, Saito H, Togashi H, Yasuda H: Fatty acid binding protein of cardiac muscle in spontaneously hypertensive rats: Effect of hypertrophy and its regression. J Moll Cell Cardiol 20: 779–787, 1988

    Article  CAS  Google Scholar 

  15. Nakanishi T, Jarmakini JM: Developmental changes in myocardial mechanical function and subcellular organelles. Am J Physiol 246: H615–625, 1988

    Google Scholar 

  16. Fabiato A, Fabiato F: Contractions induced by a calcium-triggered release of calcium from the sarcoplasmic reticulum of single skinned cardiac cells. J Physiol (London) 249: 469–495, 1975

    PubMed  CAS  Google Scholar 

  17. Glick MR, Burns AH, Reddy WJ: Dispersion and isolation of beating cells from adult rat heart. Anal Biochem 61: 32–42, 1974

    Article  PubMed  CAS  Google Scholar 

  18. Kawaguchi H, Yasuda H: Effect of elastase on prostacyclin synthesis in aortic smooth muscle cells. Biochim Biophys Acta 878: 42–48, 1986

    Article  PubMed  CAS  Google Scholar 

  19. Kawaguchi H, Yasuda H: Prostacyclin biosynthesis and phospholipase activity in hypoxic rat myocardium. Circ Res 62: 1175–1181, 1988

    Article  PubMed  CAS  Google Scholar 

  20. Kawaguchi H, Sawa F, Yasuda H: Mechanism of increased angiotensin-converting enzyme activity stimulated by platelet-activating factor. Biochim Biophys Acta 1052: 503–508, 1990

    Article  PubMed  CAS  Google Scholar 

  21. Tennes KA, McKinney JS, Putney JW: Metabolism of inositol 1, 4, 5-trisphosphate in guinea pig hepatocytes. Biochem J 242: 797–802, 1987

    PubMed  CAS  Google Scholar 

  22. Geerts H, Nuydens R, Nuyens R, Donck LV: The effect of flunarizine on intracellular calcium in isolated rat cardiomyocytes. A digital image processing study. Cardiovasc Res 23: 797–806, 1989

    CAS  Google Scholar 

  23. Wallenstein S, Zucker CL, Fleiss J: Some statistical methods useful in circulation research. Circ Res 47: 1–9, 1980

    Article  PubMed  CAS  Google Scholar 

  24. Kohl C, Schmitz W, Scholz H, Scholz J, Toth M, Doring V, Kalmar P: Evidence for α1-adrenoceptor-mediated increase of inositol triphsophate in the human heart. J Cardiovasc Pharmacol 13: 324–327.22, 1989

    Article  PubMed  CAS  Google Scholar 

  25. Renard D, Poggioli J: Does the inositol tris/tetrakisphosphate pathway exist in rat heart. FEBS lett 217: 117–123, 1987

    Article  PubMed  CAS  Google Scholar 

  26. Heathers G, Corr PB, Rubin LJ: Transient accumulation of inositol (1, 3, 4, 5)-tetrakisphosphate in response to α1-adrenergic stimulation in adult cardiac myocytes. Biochem Biophys Res Commun 156: 485–492, 1988

    Article  PubMed  CAS  Google Scholar 

  27. Simpson P: Stimulation of hypertrophy of cultured neonatal ratheart cells through an a,-adrenergic receptor and induction of beating through an α1-and β1-adrenergic receptor interaction. Circ Res 56: 884–894, 1985

    Article  PubMed  CAS  Google Scholar 

  28. Starksen NF, Simpson PC, Bishopric N, Coughlin SR, Lee WF, Escobedo JA, Williams LT: Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression. Proc Natl Acad Sci USA 83: 8348–8350, 1986

    Article  PubMed  CAS  Google Scholar 

  29. Steinberg SF, Kaplan LM, Inoue T, Zhang JF, Robinson RB: Alpha-, adrenergic stimulation of 1, 4, 5-inositol tris phosphate formation in ventricular myocytes. J Pharm Exp Ther 250: 1141–1148, 1989

    CAS  Google Scholar 

  30. Furuichi T, Yoshikawa S, Miyawaki A, Wada K, Maeda N, Mikoshiba K: Primary structure and functional expression of the inositol 1, 4, 5-trisphosphate-binding protein P400. Nature 342: 32–48, 1989

    Article  PubMed  CAS  Google Scholar 

  31. Kohl C, Schmitz W, Scholz H, Scholz J: Evidence for the existence of inositol tetrakisphosphate in mammalian heart. Effect of α1-adrenoceptor stimulation. Circ Res 66: 580–583, 1990

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, Hokkaido University School of Medicine, Sapporo, 060, Japan

    Hideaki Kawaguchi, Hitoshi Sano, Hitoshi Okada, Kenji Iizuka, Hiroshi Okamoto, Toshiyuki Kudo, Takeshi Murakami & Akira Kitabatake

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  1. Hideaki Kawaguchi
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  2. Hitoshi Sano
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  4. Kenji Iizuka
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  5. Hiroshi Okamoto
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  6. Toshiyuki Kudo
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  7. Takeshi Murakami
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  8. Akira Kitabatake
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Editors and Affiliations

  1. Third Department of Internal Medicine Faculty of Medicine, University of Tokyo, Bunkyo-ku, Hongo 7-3-1, Tokyo 113, Japan

    Yoshio Yazaki (Professor of Medicine) (Professor of Medicine)

  2. Department of Internal Medicine, Jikei University School of Medicine, Katsushika-ku, Aoto 6-41-2, Tokyo 125, Japan

    Seibu Mochizuki (Professor of Medicine) (Professor of Medicine)

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© 1993 Springer Science+Business Media Dordrecht

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Kawaguchi, H. et al. (1993). Increased Calcium Release from Sarcoplasmic Reticulum Stimulated by Inositol Trisphosphate in Spontaneously Hypertensive Rat Heart Cells. In: Yazaki, Y., Mochizuki, S. (eds) Cellular Function and Metabolism. Developments in Molecular and Cellular Biochemistry, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3078-7_8

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  • DOI: https://doi.org/10.1007/978-1-4615-3078-7_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6344-6

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