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Function and Energy Metabolism of Isolated Hearts Obtained from Hyperthyroid Spontaneously Hypertensive Rats (SHR) A 31P-Nuclear Magnetic Resonance Study

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Cellular Function and Metabolism

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 9))

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Abstract

It was the aim of this study to evaluate the effects of hyperthyroidism on heart function and cardiac energy metabolism of spontaneously hypertensive (SHR) rats. Hyperthyroidism was induced by daily injections of T3 (0.2 mg/kg s.c.) for 14 days. The hearts were then isolated and perfused in the Langendorff mode. ATP, phosphocreatine (PCr), and inorganic phosphate (Pi) were measured continuously by means of 31P-nuclear magnetic resonance (NMR) spectroscopy. Work load was altered by varying stepwise the Ca++ concentration in the perfusion fluid from 0.5 to 1.0, 1.5, and 2.0 mM, respectively. At every elevation of the Ca++ concentration, the increase in left ventricular developed pressure (LVDP) was higher in the hyperthyroid SHR than in the untreated SHR hearts. The ATP and PCr concentrations were lower in the hyperthyroid SHR compared to the untreated SHR hearts throughout the perfusion period. PCr decreased at every Ca++ elevation in both the untreated and hyperthyroid SHR hearts. The PCr/ATP ratio was not altered at any Ca++ concentration neither in the untreated SHR nor in the hyperthyroid SHR hearts. The Ca++-induced stepwise elevation in LVDP was higher at any given PCr/Pi ratio in the hyperthyroid SHR than in the untreated SHR hearts. Thus, the Ca++-inducible contractile reserve was greater in the hyperthyroid SHR heart. (Mol Cell Biochem 119: 43–50, 1993)

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Authors and Affiliations

  1. Department of Physiology, University of Munich, Germany

    M. Heckmann & H. G. Zimmer

  2. Laboratoire de Physiologie Cellulaire Cardiaque, Université Joseph Fourier, Grenoble, France

    S. Lortet, J. Aussedat, A. Ray & A. Rossi

Authors
  1. M. Heckmann
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  2. S. Lortet
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  3. J. Aussedat
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  4. A. Ray
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  5. A. Rossi
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  6. H. G. Zimmer
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Editors and Affiliations

  1. Third Department of Internal Medicine Faculty of Medicine, University of Tokyo, Bunkyo-ku, Hongo 7-3-1, Tokyo 113, Japan

    Yoshio Yazaki (Professor of Medicine) (Professor of Medicine)

  2. Department of Internal Medicine, Jikei University School of Medicine, Katsushika-ku, Aoto 6-41-2, Tokyo 125, Japan

    Seibu Mochizuki (Professor of Medicine) (Professor of Medicine)

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© 1993 Springer Science+Business Media Dordrecht

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Heckmann, M., Lortet, S., Aussedat, J., Ray, A., Rossi, A., Zimmer, H.G. (1993). Function and Energy Metabolism of Isolated Hearts Obtained from Hyperthyroid Spontaneously Hypertensive Rats (SHR) A 31P-Nuclear Magnetic Resonance Study. In: Yazaki, Y., Mochizuki, S. (eds) Cellular Function and Metabolism. Developments in Molecular and Cellular Biochemistry, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3078-7_7

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  • DOI: https://doi.org/10.1007/978-1-4615-3078-7_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6344-6

  • Online ISBN: 978-1-4615-3078-7

  • eBook Packages: Springer Book Archive

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