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Three Dimensional Structure of Acetylcholinesterase

  • Chapter
Multidisciplinary Approaches to Cholinesterase Functions

Abstract

Acetylcholinesterase (AChE, acetylcholine hydrolase, EC 3.1.1.7) plays a key role in cholinergic neurotransmission. By rapid hydrolysis of the transmitter acetylcholine (ACh), the enzyme terminates the chemical impulse, thereby permitting rapid repetitive responses and allowing re-uptake of choline (Katz, 1966; Barnard, 1974):

CH3COOCH2CH2N+(CH3)3 + AChE

↓

CH3CO-AChE + HOCH2CH2N+(CH3)3

↓

CH3COO-+ H+ + AChE

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References

  • Amitai, G., and Taylor, P., 1991, in: “Cholinesterases: Structure, Function, Mechanism, Genetics and Cell Biology,” J. Massonlié, F. Bacou, E. Barnard, A. Chatonnet, B. P. Doctor, and D. M. Quinn, Eds, American Chemical Society, Washington, DC.

    Google Scholar 

  • Barnard, E. A., 1974, in: “The Peripheral Nervous System,” J. I. Hubbard, Eds, Plenum, New York.

    Google Scholar 

  • Bazelyansky, M., Robey, C., and Kirsch, J. F., 1986, Biochemistry. 25:125.

    Article  PubMed  CAS  Google Scholar 

  • Belleau, B., DiTullio, V., and Tsai, Y. H., 1970, Mol. Pharmacol. 6:41.

    PubMed  CAS  Google Scholar 

  • Bergmann, F., Wilson, I. B., and Nachmansohn, D., 1950, Biochim. Biophys. Acta. 6:217.

    Article  PubMed  CAS  Google Scholar 

  • Berman, H. A., and Decker, M. M., 1989, J Biol. Chem. 264:3951.

    PubMed  CAS  Google Scholar 

  • Berman, H. A., and Leonard, K., 1989, J. Biol. Chem. 264:3942.

    PubMed  CAS  Google Scholar 

  • Berman, H. A., and Leonard, K., 1990, Biochemistry. 29:10640.

    Article  PubMed  CAS  Google Scholar 

  • Bernstein, F. C., Koetzel, T. F., Williams, G. J. B., Meyer, E. F., Jr., Brice, M. D., Rodgers, J. R., Kennard, O., Schimanouchi, T., and Tasunmi, M., 1977, JMB. 112:535.

    Article  CAS  Google Scholar 

  • Blumberg, S., and Silman, I., 1978, Biochemistry. 17:1125.

    Article  PubMed  CAS  Google Scholar 

  • Burley, S. K., and Petsko, G. A., 1985, Science. 229:23.

    Article  PubMed  CAS  Google Scholar 

  • Changeux, J. P., 1966, Mol. Pharmacol. 2:369.

    PubMed  CAS  Google Scholar 

  • Chothia, C., and Pauling, P., 1968, Nature. 219:1156.

    Article  PubMed  CAS  Google Scholar 

  • Cygler, M., Schrag, J. D., Sussman, J. L., Harel, M., Silman, I., Gentry, M. K., and Doctor, B. P., 1992 (submitted).

    Google Scholar 

  • Doctor, B. P., Smyth, K. K., Gentry, M. K., Ashani, Y., Christner, C. E., De La Hoz, D. M., Ogert, R. A., and Smith, S. W., 1989, in: “Computer-Assisted Modeling of Receptor-Ligand Interactions. Theoretical Aspects and Applications to Drug Design,” R. Rein, and A. Golombek, Eds, A.R. Liss, New York.

    Google Scholar 

  • Dougherty, D. A., and Stauffer, D. A., 1990, Science. 250:1558.

    Article  PubMed  CAS  Google Scholar 

  • Drachman, D., Ed, 1987, “Myasthenia gravis: biology and treatment,” vol. 555 of “Ann. N.Y. Acad. Sci.,” New York.

    Google Scholar 

  • Franken, S. M., Rozeboom, H. J., Kalk, K. H., and Dijkstra, B. W., 1991, EMBO J. 10:1297.

    PubMed  CAS  Google Scholar 

  • Freeman, S. E., and Dawson, R. M., 1991, Prog. Neurobiol. 36:257.

    Article  PubMed  CAS  Google Scholar 

  • Fuchs, S., Gurari, D., and Silman, I., 1974, Arch. Biochem. Biophys. 165:90.

    Article  PubMed  CAS  Google Scholar 

  • Galzi, J.-C., Revah, F., Bouet, F., Ménez, A., Goeldner, M., Hirth, C., and Changeux, J.-P., 1991, Proc. Natl. Acad. Sci. USA. 88:5051.

    Article  PubMed  CAS  Google Scholar 

  • Gauthier, S., and Gauthier, L., 1991, in: “Cholinergic Basis of Alzheimer Therapy,” R. Becker, and E. Giacobini, Eds, Birkhauser, Berlin.

    Google Scholar 

  • Gentry, M. K., and Doctor, B. P., 1991, in: “Cholinesterases: Structure, Function, Mechanism, Genetics and Cell Biology,” J. Massoulié, F. Bacou, E. Barnard, A. Chatonnet, B. P. Doctor, and D. M. Quinn, Eds, American Chemical Society, Washington, DC.

    Google Scholar 

  • Gibney, G., Camp, S., Dionne, M., MacPhee-Quigley, K., and Taylor, P., 1990, Proc. Natl. Acad. Sci. USA. 87:7546.

    Article  PubMed  CAS  Google Scholar 

  • Goeldner, M. P., and Hirth, C. G., 1980, Proc. Natl. Acad. Sci. USA. 77:6439.

    Article  PubMed  CAS  Google Scholar 

  • Gubemator, K., Müller, K., and Winkler, F. K., 1991, in: “Lipases- Structure, Mechanism and Genetic Engineering,” L. Alberghina, R. D. Schmid, and R. Verger, Eds, VCH, Weinheim.

    Google Scholar 

  • Hallak, M., and Giacobini, E., 1989, Neuropharmacology. 28:199.

    Article  PubMed  CAS  Google Scholar 

  • Hartmann, H. A., Kirsch, G. E., Drewe, J. A., Taglialatela, M., Joho, R. H., and Brown, A. M., 1991, Science. 251:942.

    Article  PubMed  CAS  Google Scholar 

  • Hasinoff, B. B., 1982, Biochim. Biophys. Acta. 704:52.

    Article  PubMed  CAS  Google Scholar 

  • Heilbronn, E., 1961, Acta Chem. Scand. 15:1386.

    Article  CAS  Google Scholar 

  • Hobbiger, F., 1952, Brit. J. Pharmacol. 7:223.

    PubMed  CAS  Google Scholar 

  • Kabachnik, M. I., Brestkin, A. P., Godovikov, N. N., Michelson, M. J., Rozengart, E. V., and Rozengart, V. I., 1970, Pharmacol. Rev. 22:355.

    PubMed  CAS  Google Scholar 

  • Katz, B., 1966, “Nerve, Muscle and Synapse,” McGraw-Hill, New York.

    Google Scholar 

  • Kieffer, B., Goeldner, M., Hirth, C., Aebersold, R., and Chang, J. Y., 1986, FEBS Lett. 202:91.

    Article  CAS  Google Scholar 

  • Koelle, G. B., Ed, 1963, “Cholinesterase and anti-cholinesterase agents,” vol. 15 of “Handbuch der Experimentellen Pharmakologie,” Springer-Verlag, Heidelberg.

    Google Scholar 

  • Levitt, M., and Chothia, C., 1976, Nature. 261:552.

    Article  PubMed  CAS  Google Scholar 

  • Liao, D., and Remington, S. J., 1990, J. Biol. Chem. 265:6528.

    PubMed  CAS  Google Scholar 

  • MacKinnon, R., 1991, Current Opinion Neurobiol. 1:14.

    Article  CAS  Google Scholar 

  • MacPhee-Quigley, K., Taylor, P., and Taylor, S., 1985, J. Biol. Chem. 260:12185.

    PubMed  CAS  Google Scholar 

  • Massoulié, J., Sussman, J. L., Doctor, B. P., Soreq, H., Velan, B., Cygler, M., Rotundo, R., Shafferman, A., Silman, I., and Taylor, P., 1992, this volume.

    Google Scholar 

  • Meunier, J.-C., and Changeux, J.-P., 1969, FEBS Lett. 2:224.

    Article  PubMed  CAS  Google Scholar 

  • O’Brien, R. D., 1969, Biochem. J. 113:713.

    PubMed  Google Scholar 

  • Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S. M., Hare!, M., Remington, S. J., Silman, I., Schrag, J., Sussman, J. L., Verschueren, K. H. G., and Goldman, A., 1992, Protein Engineering. 5:197.

    Article  PubMed  CAS  Google Scholar 

  • Osserman, K. E., and Genkins, G., 1971, Mt. Sinai J. Med. 38:497.

    PubMed  CAS  Google Scholar 

  • Page, J. D., and Wilson, I. B., 1985, J. Biol. Chem. 260:1475.

    PubMed  CAS  Google Scholar 

  • Pathak, D., Ngai, K. L., and Ollis, D., 1988, JMB. 204:435.

    Article  CAS  Google Scholar 

  • Pathak, D., and Ollis, D., 1990, JMB. 214:497.

    Article  CAS  Google Scholar 

  • Purdie, J. E., and McIvor, R. A., 1966, Biochim. Biophys. Acta. 128:590.

    Article  CAS  Google Scholar 

  • Quinn, D. M., 1987, Chem. Rev. 87:955.

    Article  CAS  Google Scholar 

  • Radic, Z., Reiner, E., and Taylor, P., 1991, Mol. Pharmacol. 39:98.

    PubMed  CAS  Google Scholar 

  • Reiner, E., Aldridge, N., Simeon, V., Radic, Z., and Taylor, P., 1991, in: “Cholinesterases: Structure, Function, Mechanism, Genetics and Cell Biology,” J. Massonlié, F. Bacou, E. Barnard, A. Chatonnet, B. P. Doctor, and D. M. Quinn, Eds, American Chemical Society, Washington, DC.

    Google Scholar 

  • Richardson, J. S., 1981, Adv. Protein Chem. 34:167.

    Article  PubMed  CAS  Google Scholar 

  • Richardson, J. S., 1985, in:“Diffraction Methods for Biological Macromolecules,” H. W. Wyckoff, C. H. W. Hirs, and S. N. Timasheff, Eds, Academic Press, New York.

    Google Scholar 

  • Rosenberry, T. L., 1975, Adv. Enrymol. 43:103.

    CAS  Google Scholar 

  • Schrag, J. D., Li, Y., Wu, S., and Cygler, M., 1991, Nature. 351:761.

    Article  PubMed  CAS  Google Scholar 

  • Shafferman, A., Kronman, C., Flashner, Y., Leitner, M., Grosfeld, H., Ordentlich, A., Gozes, Y., Cohen, S., Ariel, N., Barak, D., Hare!, M., Silman, I., Sussman, J. L., and Velan, B., 1992a, J. Biol. Chem. (in press).

    Google Scholar 

  • Shafferman, A., Velan, B., Ordentlich, A., Kronman, C., Grosfeld, H., Lehner, M., Flashner, Y., Cohen, S., Barak, D., and Arid, N., 1992b, this volume.

    Google Scholar 

  • Shinitzky, M., Dudai, Y., and Silman, I., 1973, FEBS Lett. 30:125.

    Article  PubMed  CAS  Google Scholar 

  • Silman, I., and Futerman, A. H., 1987, Eur. J. Biochem. 170:11.

    Article  PubMed  CAS  Google Scholar 

  • Slabas, A. R., Windust, J., and Sidebottom, C. M., 1990, Biochem. J. 269:279.

    PubMed  CAS  Google Scholar 

  • Steinberg, G. M., Mednick, M. L., Maddox, J., Rice, R., and Cramer, J., 1975, J. Med. Chem. 18:1056.

    Article  CAS  Google Scholar 

  • Steitz, T. A., and Shulman, R. G., 1982, Annu. Rev. Biophys. Bioeng. 11:419.

    Article  PubMed  CAS  Google Scholar 

  • Summers, W. K., Majovski, L. V., Marsch, G. M., Tachiki, K., and Kling, A., 1986, New England J. Med. 315:1241.

    Article  CAS  Google Scholar 

  • Sussman, J. L., Harel, M., Frolow, F., Oefner, C., Goldman, A., Toker, L., and Silman, I., 1991, Science. 253:872.

    Article  PubMed  CAS  Google Scholar 

  • Sussman, J. L., Harel, M., Frolow, F., Varon, L., Toker, L., Futerman, A. H., and Silman, I., 1988, JMB. 203:821.

    Article  CAS  Google Scholar 

  • Taylor, P., 1990, in: “The Pharmacological Basis of Therapeutics,” A. G. Gilman, A. S. Nies, T. W. Rall, and P. Taylor, Eds, MacMillan, New York.

    Google Scholar 

  • Weise, C., Kreienkamp, H.-J., Raba, R., Pedak, A., Aaviksaar, A., and Hucho, F., 1990, EMBO J. 9:3885.

    CAS  Google Scholar 

  • Wilson, I. B., and Quan, C., 1958, Arch. Biochem. Biophys. 73:131.

    Article  PubMed  CAS  Google Scholar 

  • Winkler, F. K., D’Arcy, A., and Hunziker, W., 1990, Nature. 343:771.

    Article  PubMed  CAS  Google Scholar 

  • Yool, A. J., and Schwartz, T. L., 1991, Nature. 349:700.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Structural Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    J. L. Sussman & M. Harel

  2. Department of Neurobiology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    I. Silman

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  1. J. L. Sussman
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  2. M. Harel
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  3. I. Silman
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Editors and Affiliations

  1. Israel Institute for Biological Research, Ness-Ziona, Israel

    Avigdor Shafferman  & Baruch Velan  & 

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Sussman, J.L., Harel, M., Silman, I. (1992). Three Dimensional Structure of Acetylcholinesterase. In: Shafferman, A., Velan, B. (eds) Multidisciplinary Approaches to Cholinesterase Functions. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3046-6_14

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  • DOI: https://doi.org/10.1007/978-1-4615-3046-6_14

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