Abstract
Multiple findings implicate acetylcholine with sperm functioning 1,2 and acetyl-and butyrylcholinesterase activities (ACHE, BCHE) were observed in mammalian sperm cells and during oocyte development 1–3. In vivo amplification of the human BCHE gene was first found in a father and son exposed to cholinesterase inhibitors 4, but it remained unclear whether the amplified DNA was transmitted as such from father to son or whether the amplification phenomenon re-occurred in germ cells, particularly during male meiosis or sperm differentiation.
Keywords
- Transgenic Mouse
- Cholinesterase Inhibitor
- Sperm Differentiation
- Butyrylcholinesterase Activity
- Endogenous Ache
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
B.V. Rama Sastry, V.E. Janson and A.K. Chaturvedi. Inhibition of human sperm motility by inhibitors of choline acetyltransferase. J. Pharmacol. Exp. Ther. 216: 378–384 (1981).
C.F. Ibanez, M. Pelto-Huikko, O. Soder, et al. Expression of choline acetyltransferase mRNA in spermatogenic cells results in an accumulation of the enzyme in the postacrosomal region of mature spermatozoa. Proc. Natl. Acad. Sci. USA. 88: 3676–3680 (1991).
G. Malinger, H. Zakut and H. Soreq. Cholinoceptive properties of human primordial, pre-antral and mature oocytes: In-situ hyridization and biochemical evidence for expression of cholinesterase genes. J. Mol. Neuroscience 1: 77–84 (1989).
C.A. Prody, P. Dreyfus, R. Zamir, et al. De-novo amplification within a “silent” human cholinesterase gene in a family subjected to prolonged exposure to organophosphorous insecticides. Proc. Natl. Acad. Sci. USA. 86: 690–694 (1989).
R.T. Schimke, Gene amplification in cultured animal cells. Cell 37:705–713 (1984).
J.M. Bishop, The molecular genetics of cancer. Science 235:305–311(1987).
P.G. Pauw, M.D. Johnoson, P. Moore, et al. Stable gene amplification and overexpression of sodium and potassium activated ATPase in Hela cells. Mol. Cell. Biol. 6: 1164–1171 (1986).
Y. Lapidot-lifson, C.A. Prody, D. Ginzberg, et al. Co-amplification of human acetylcholinesterase and butyrylcholin-esterase genes in blood cells: correlation with various leukemias and abnormal megakaryocytopoiesis. Proc. Natl. Sci. USA. 86:4715–4719 (1989).
H. Zakut, G.Ehrlich, A.Ayalon, et al. Acetylcholinesterase and butyrylcholinesterase genes coamplify in primary ovarian carcinomas. J. Clin. Invest. 86: 900–908 (1990).
H. Zakul, Y. Lapidot-Lifson, R. Beeri, et al. In-vivo gene amplification in non cancerous cells: cholinesterase genes and oncogenes amplify in thrombocytopenia associated with Lupus erythematosus. Mutation Research, in press. (1992).
M. Shani. Tissue specific expression of rat myosin light chain 3 gene in transgenic mice. Nature 314: 283–286 (1985).
R. Beeri, A. Gnatt, Y. Lapidot-Lifson, et al. Gene amplification and its impaired transmission studied in transgenic mice canying human butyrylcholinesterase cDNA. submitted (1992).
E. Kim, S.H. Waters, L.E. Hake, et al. Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice. Mol. Cell Biol. 1875 1881 (1989).
Y. Lapidot-Lifson, D. Patinkin, C.A. Prody, et al. Cloning and antisense oligodeoxynucleotide inhibition of a human homolog of cdc2 required in hematopoiesis. Proc. Natl. Acad. Sci. USA, 89:579–583 (1992).
C.A. Prody, D. Zevin-Sonkin, A. Gnatt, et al. Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues. Proc. Natl. Acad. Sci. USA, 84:3555–3559 (1987).
P.G. Layer, Cholinesterases during development of the avian nervous system. Cell. Mol. Neurobiol. 11:7–33. (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Beeri, R. et al. (1992). Testicular Gene Amplification and Impaired BCHE Transcription Induced in Transgenic Mice by the Human BCHE Coding Sequence. In: Shafferman, A., Velan, B. (eds) Multidisciplinary Approaches to Cholinesterase Functions. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3046-6_13
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3046-6_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6328-6
Online ISBN: 978-1-4615-3046-6
eBook Packages: Springer Book Archive