Skip to main content
SpringerLink
Log in

Attachment and Colonization of Pseudomonas aeruginosa: Role of the Surface Structures

  • Chapter
Pseudomonas aeruginosa as an Opportunistic Pathogen

Part of the book series: Infectious Agents and Pathogenesis ((IAPA))

Abstract

Cystic fibrosis (CF) patients frequently suffer from chronic Pseudomonas aeruginosa pulmonary infections.1–3 These pulmonary infections are thought to be initiated by the attachment to and subsequent colonization of the mucosal epithelium of the upper respiratory tract by P. aeruginosa and followed by a descending infection mechanism.2,4–6 The initial pulmonary infection is generally due to nonmucoid strains of P. aeruginosa, 7–10 but following the initial infection mucoid strains predominate and are isolated in 50%11 to 90%12 of all CF patients. Increasing severity of the pulmonary infection appears to be correlated with the appearance and predominance of mucoid strains of P. aeruginosa in the sputum of CF patients.1,7,8,10 Significantly, phenotypic switching of a nonmucoid to mucoid phenotype has been noted in an in vivo chronic lung infection model without antibiotic select ion,13 which suggests that the mucoid phenotype is not selected for by antibiotic utilization.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 16.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Doggett, R. G., 1979, Pseudomonas aeruginosa: Clinical Manifestations of Infection and Current Therapy, Academic Press, New York.

    Google Scholar 

  2. Gilligan, P. H., 1991, Microbiology of airway disease in patients with cystic fibrosis, Clin. Microbiol. Rev. 4:35–51.

    PubMed  CAS  Google Scholar 

  3. Wood, R. E., Boat, T. E., and Doershuk, C. E., 1976, Cystic fibrosis, Am. Rev. Respir. Dis. 113: 833–878.

    PubMed  CAS  Google Scholar 

  4. Johanson, W. G., Jr., Higuchi, J. H., Chaudhuri, T. Woods, D. E., 1980, Bacterial adherence to epithelial cells in the bacillary colonization of the respiratory tract, Am. Rev. Resp. Dis. 121: 55–63.

    PubMed  Google Scholar 

  5. Johanson, W. G., Jr., Pierce, A. K., Sanford, J. P., and Thomas, G. D., 1972, Nosocomial respiratory infections with gram-negative bacilli. The significance of colonization of the respiratory tract, Ann. Intern. Med. 77:701–706.

    PubMed  Google Scholar 

  6. Johanson, W. G., Jr., Woods, D. E., and Chaudhuri, T., 1979, Association of respiratory tract colonization with adherence of gram-negative bacilli to epithelial cells, J. Infect. Dis. 139: 667–673.

    Article  PubMed  Google Scholar 

  7. Doggett, R. G., 1969, Incidence of mucoid Pseudomonas aeruginosa from clinical sources, Appl. Microbiol. 18:936–937.

    PubMed  CAS  Google Scholar 

  8. Høiby, N., 1974, Pseudomonas aeruginosa infection in cystic fibrosis. Relationship between mucoid strains of Pseudomonas aeruginosa and humoral immune response, Acta Pathol. Microbiol. Scand. Sect. B 82:551–558.

    Google Scholar 

  9. Høiby, N., 1975, Prevalence of mucoid strains of Pseudomonas aeruginosa in bacteriological specimens from patients with cystic fibrosis and patients without cystic fibrosis, Acta Pathol. Microbiol. Scand. Sect. B 83:321–327.

    Google Scholar 

  10. Høiby, N., 1982, Microbiology of lung infections in cystic fibrosis patients, Acta Paediatr. Scand. 301 (Suppl.):33–54.

    Article  Google Scholar 

  11. Kulczycki, L. L., Murphy, T. M., and Bellanti, J. A., 1978, Pseudomonas aeruginosa colonization in cystic fibrosis. A study of 160 patients, J. Am. Med. Assoc. 240:30–34.

    Article  CAS  Google Scholar 

  12. Luraya-Cussay, L. R., Cundy, K. R., and Huang, N. N., 1976, Pseudomonas carrier rates of patients with cystic fibrosis and members of their families, J. Pediatr. 89:23–26.

    Article  Google Scholar 

  13. Woods, D. E., Sokol, P. A., Bryan, L. E., Storey, D. G., Mattingly, J., Vogel, H. J., and Ceri, H., 1991, In vivo regulation of virulence in Pseudomonas aeruginosa associated with genetic rearrangement, J. Infect. Dis. 163:143–149.

    Article  PubMed  CAS  Google Scholar 

  14. Todd, T., Franklin, A. L., Gurman, G., Mankinen-Irvin, P. M., and Irvin, R. T., 1989, Augmented bacterial adherence to ciliated tracheal epithelial cells in an intensive care unit population, Am. Rev. Respir. Dis. 140:1585–1589.

    Article  PubMed  CAS  Google Scholar 

  15. Niederman, M. S., Merrill, W. W., Ferranti, R. D., Pagano, K. W., Palmer, L. B., and Reynolds, H. Y., 1984, Nutritional status and bacterial binding in the lower respiratory tract in patients with chronic tracheostomy, Ann. Int. Med. 100:795–800.

    PubMed  CAS  Google Scholar 

  16. Niederman, M.. S., Merrill, W. W., Polomski, L. M., Reynolds, H. Y., and Gee, J. B. L., 1986, Influence of sputum IgA and elastase on tracheal cell bacterial adherence, Am. Rev. Resp. Dis. 144:255–260.

    Google Scholar 

  17. Niederman, M. S., Rafferty, T. D., Sasaki, C. T., Merrill, W. W., Matthay, R. A., and Reynolds, H. Y., 1983, Comparison of bacterial adherence to ciliated and squamous epithelial cells obtained from the human respiratory tract, Am. Rev. Respir. Dis. 127:85–90.

    CAS  Google Scholar 

  18. Laibson, P. R., 1972, Annual review: Cornea and sclera, Arch. Ophthalmol. 88:553–574.

    Article  PubMed  CAS  Google Scholar 

  19. Ramphal, R., McNiece, M.. T., and Polack, F. M., 1981, Adherence of Pseudomonas aeruginosa to the injured cornea: A step in the pathogenesis of corneal infections, Ann. Ophthalmol. 13: 421–425.

    PubMed  CAS  Google Scholar 

  20. Hazlet, L. D., Moon, M., and Berk, R. S., 1986, In vivo identification of Hazlet, L. D., Moon, M., and Berk, R. S., 1986, In vivo identification of sialic acid as the ocular receptor for Pseudomonas aeruginosa, Infect. Immun. 51:687–689.

    Google Scholar 

  21. Stern, G. A., Weitzenkorn, D., and Valenti, J., 1982, Adherence of Pseudomonas aeruginosa to the mouse cornea: Epithelial vs stromal adherence, Arch. Ophthalmol 100:1956.

    Article  PubMed  CAS  Google Scholar 

  22. Hazlet, L. D., Rosen, D. D., and Berk, R. S., 1978, Age-related susceptibility to Pseudomonas aeruginosa ocular infections in mice, Infect. Immun. 20:25–29.

    Google Scholar 

  23. Reichert, R.. W, Das, N. D., and Zam, Z. S., 1983, Adherence properties of Pseudomonas pili to epithelial cells of the human cornea, Gurr. Eye Res. 2:289–293.

    CAS  Google Scholar 

  24. Rudner, X. L., Zheng, Z., Berk, R. S., Irvin, R. T., and Hazlett, L. D., 1991, Corneal epithelial glycoproteins exhibit P. aeruginosa pilus binding activity, submitted.

    Google Scholar 

  25. Doig, P., Smith, N. R., Todd, T., and Irvin, R. T., 1987, Characterization of the binding of Pseudomonas aeruginosa alginate to human epithelial cells, Infect. Immun. 55:1517–1522.

    PubMed  CAS  Google Scholar 

  26. Doig, E, Tapping, R., Mankinen-Irvin, P., and Irvin, R. T., 1989, Effect of microcolony formation on the adherence of Pseudomonas aeruginosa to human buccal epithelial cells, Microb. Ecol. Health Dis. 2:203–209.

    Article  Google Scholar 

  27. Doig, P, Todd, T., Sastry, P. A., Lee, K. K. Hodges, R. S., Paranchych, W, and Irvin, R. T., 1988, Role of pili in the adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells, Infect. Immun. 56:1641–1646.

    PubMed  CAS  Google Scholar 

  28. McEachran, D. W., and Irvin, R. T., 1985, Adhesion of Pseudomonas aeruginosa to human buccal epithelial cells: Evidence for two classes of receptors, Can. J. Microbiol. 31:563–569.

    Article  PubMed  CAS  Google Scholar 

  29. McEachran, D. W., and Irvin, R. T., 1986, A new method for the irreversible attachment of cells or proteins to polystyrene tissue culture plates for use in the study of bacterial adhesion, J. Microbiol. Methods 5:99–111.

    Article  CAS  Google Scholar 

  30. Zoutman, D. E., Hulbert, W. C., Pasloske, B. L., Joffe, A. M., Volpel, K., Trebilock, M. K., and Paranchych, W, 1991, The role of polar pili in the adherence of Pseudomonas aeruginosa to injured canine tracheal epithelial cells: A semiquantitative morphologic study, Scanning Electron Microsc. 5:109–126.

    CAS  Google Scholar 

  31. Franklin, A. L., Todd, T., Gurman, G., Black, D., Mankinen-Irvin, P. M., and Irvin, R. T., 1987, Adherence of Pseudomonas aeruginosa to cilia of human tracheal epithelial cells, Infect. Immun. 55:1523–1525.

    PubMed  CAS  Google Scholar 

  32. Baker, N. R., Minor, V., Deal, C., Sahhrabadi, M. S., Simpson, D. A., and Woods, D. E., 1991, Pseudomonas aeruginosa exoenzyme S is an adhesin, Infect. Immun. 59:2859–2863.

    PubMed  CAS  Google Scholar 

  33. Ramphal, R., and Pyle, M., 1983, Evidence for mucins and sialic acid as receptors for Pseudomonas aeruginosa in the lower respiratory tract, Infect. Immun. 41:339–344.

    PubMed  CAS  Google Scholar 

  34. Ramphal, R., and Pier, G. B., 1985, Role of Pseudomonas aeruginosa mucoid exopolysaccharide in adherence of tracheal cells, Infect. Immun. 47:1–4.

    PubMed  CAS  Google Scholar 

  35. Ramphal, R., Sadoff, J. C., Pyle, M., and Silipigni, J. D., 1984, Role of pili in the adherence of Pseudomonas aeruginosa to injured tracheal epithelium, Infect. Immun. 44:38–40.

    PubMed  CAS  Google Scholar 

  36. Woods, D. E., Straus, D. C., Johanson, W. G., Jr., Berry, V. K., and Bass, J. A., 1980, Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells, Infect. Immun. 29:1146–1151.

    PubMed  CAS  Google Scholar 

  37. Doig, P., Sastry, P. A., Hodges, R. S., Lee, K. K., Paranchych, W, and Irvin, R. T., 1990, Inhibition of pilus-mediated adhesion of Pseudomonas aeruginosa to human buccal epithelial cells by monoclonal antibodies directed against pili, Infect. Immun. 58:124–130.

    PubMed  CAS  Google Scholar 

  38. Irvin, R. T., Doig, P. C., Sastry, P. A., Heller, B., and Paranchych, W, 1989, Competition for bacterial receptor sites on respiratory epithelial cells by Pseudomonas aeruginosa strains of heterologous pilus type: Usefulness of kinetic parameters in predicting the outcome, Microb. Ecol. Health Dis. 3:39–47.

    Article  Google Scholar 

  39. Linker, A., and Jones, R. S., 1966, A new polysaccharide resembling alginic acid isolated from Pseudomonas, J. Biol. Chem. 241:3845–3851.

    PubMed  CAS  Google Scholar 

  40. Evans, L.R., and Linker, A., 1973, Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa, J. Bacteriol. 116:915–924.

    PubMed  CAS  Google Scholar 

  41. Sutherland, I. W., 1977, Bacterial exopolysaccharides. Their nature and production. In: Surface Carbohydrates of the Prokaryotic Cell (I. W. Sutherland, ed.), Academic Press, London, pp. 27–96.

    Google Scholar 

  42. Cheng, K-J., Irvin, R. T., and Costerton, J. W, 1981, Autochthonous and pathogenic colonization of animal tissues by bacteria, Can. J. Microbiol. 27:461–490.

    Article  PubMed  CAS  Google Scholar 

  43. Costerton, J. W., Irvin, R. T., and Cheng, K-J., 1981, The bacteria glycocalyx in nature and disease, Ann. Rev. Microbiol. 35:299–324.

    Article  CAS  Google Scholar 

  44. Costeron, J. W., Irvin, R. T., and Cheng, K-J., 1981, The role of bacterial surface structures in pathogenesis, CRC Grit. Rev. Microbiol. 8:303–338.

    Article  Google Scholar 

  45. Govan, J. R. W, and Fyfe, J. A. M., 1978, Mucoid Pseudomonas aeruginosa and cystic fibrosis: Resistance of the mucoid form to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro, J. Antimicrob. Chemother. 4:233–240.

    Article  PubMed  CAS  Google Scholar 

  46. Schwarzmann, S., and Boring, J. R., III, 1971, Antiphagocytic effect of slime from a mucoid strain of Pseudomonas aeruginosa,Infect. Immun. 3:762–767.

    PubMed  CAS  Google Scholar 

  47. Baltimore, R. S., and Mitchell, M., 1980, Immunologic investigations of mucoid strains of Pseudomonas aeruginosa: Comparison of susceptibility to opsonic antibody in mucoid and nonmucoid strains, j Infect. Dis. 141:238–247.

    Article  PubMed  CAS  Google Scholar 

  48. Ruhen, R. W., Holt, P. G., and Padadimitriou, J. M., 1980, Antiphagocytic effect of Pseudomonas aeruginosa exopolysaccharide, J. Clin. Pathol. 33:1221–1222.

    Article  PubMed  CAS  Google Scholar 

  49. Meshulam, T., Obedeanu, N., Merzbach, D., and Sobel, J. D., 1984, Phagocytosis of mucoid and nonmucoid strains of Pseudomonas aeruginosa, Clin. Immun. Immunopathol. 32:151–165.

    Article  CAS  Google Scholar 

  50. Ames, P., DesJardins, D., and Pier, G. B., 1985, Opsonophagocytic killing activity of rabbit antibody to Pseudomonas aeruginosa mucoid exopolysaccharide, Infect. Immun. 49:281–285.

    PubMed  CAS  Google Scholar 

  51. Woods, D. E., and Bryan, L. E., 1985, Studies on the ability of alginate to act as a protective immunogen against infection with Pseudomonas aeruginosa in animals, 151:581–588.

    CAS  Google Scholar 

  52. Govan, J. R. W., Fyfe, J. A. M., and Baker, N. R., 1983, Heterogeneity and reduction in pulmonary clearance of mucoid Pseudomonas aeruginosa, Rev. Infect. Dis. 5:S874—S875.

    Google Scholar 

  53. Krieg, D. E., Bass, J. A., and Mattingly, S. J., 1986, Aeration selects for mucoid phenotype of Pseudomonas aeruginosa,J. Clin. Microbiol. 24:986–990.

    PubMed  CAS  Google Scholar 

  54. Learn, D. B., Brestel, E. E., and Seetharama, S., 1987, Hypochlorite scavenging by Pseudomonas aeruginosa alginate, Infect. Immun. 55:1813–1818.

    PubMed  CAS  Google Scholar 

  55. Darzins, A., and Chakrabarty, A. M., 1984, Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa, J. Bacteriol. 159: 9–18.

    PubMed  CAS  Google Scholar 

  56. Deretic, V, and Konyecsni, W M., 1990, A procaryotic regulatory factor with a histone H1-like carboxy-terminal domain: Clonal variation of repeats within algP, a gene involved in regulation of mucoidy in Pseudomonas aeruginosa,J. Bacteriol. 172:5544–5554.

    PubMed  CAS  Google Scholar 

  57. Kato, J., Misra, T K., and Chakrabarty, A. M., 1990, A1gR3, a protein resembling eukaryotic histone H1, regulates alginate synthesis in Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. USA 87:2887–2891.

    Article  PubMed  CAS  Google Scholar 

  58. Konyecsni, W. M., and Deretic, V., 1990, DNA sequence and expression analysis of algP and algQ, components of the multigene system transcriptionally regulating mucoidy in Pseudomonas aeruginosa: algP contains multiple direct repeats, J. Bacteriol. 172:2511–2520.

    PubMed  CAS  Google Scholar 

  59. Mohr, C. D., and Deretic, V., 1990, Gene-scrambling mutagenesis: generation and analysis of insertional mutations in the alginate regulatory region of Pseudomonas aeruginosa, J. Bacteriol. 172:6252–6260.

    PubMed  CAS  Google Scholar 

  60. Mohr, C. D., Martin, D. W., Konyecsni, W. M., Govan, J. R. W, Lory, S., and Deretic, V, 1990, Role of the far-upstream sites of the algD promoter and the algR and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa, J. Bacteriol. 172:6576–6580.

    PubMed  CAS  Google Scholar 

  61. Pier, G. B., DesJardin, D., Aguilar, T., Barnard, M., and Speert, D., 1986, Polysaccharide surface antigens expressed by nonmucoid isolates of Pseudomonas aeruginosa from cystic fibrosis patients, J. Clin. Microbial. 24:189–196.

    CAS  Google Scholar 

  62. Pugashetti, K. B., Metzger, H. M., Jr., Vadas, L., and Feingold, D. S., 1982, Phenotypic differences among clinically isolated mucoid Pseudomonas aeruginosa strains, J. Clin. Microbiol. 16:686–691.

    PubMed  CAS  Google Scholar 

  63. Piggott, N. H., Sutherland, I. W., and Jarman, T. R., 1982, Alginate synthesis by mucoid strains of Pseudomonas aeruginosa PAO, Eur. J. Appl. Microbiol. Biotechnol. 16:131–135.

    Article  CAS  Google Scholar 

  64. Piggott, N. H., Sutherland, I. W., and Jarman, T. R., 1982, Enzymes involved in the biosynthesis of alginate by Pseudomonas aeruginosa, Eur. J.Appi. Microbiol. Biotechnol. 13: 179–183.

    Article  Google Scholar 

  65. McArthur, H. A. I., and Ceri, H., 1983, Interaction of a rat lung lectin with the exopolysaccharides of Pseudomonas aeruginosa, Infect. Immun. 42:574–578.

    PubMed  CAS  Google Scholar 

  66. Sherbrock-Cox, V., Russell, N. J., and Gacesa, P., 1984, The purification and chemical characterization of the alginate present in the extracellular material produced by mucoid strains of Pseudomonas aeruginosa, Carbohydr. Res. 135:147–154.

    Article  PubMed  CAS  Google Scholar 

  67. Pier, G. B., Matthews, W. J., and Eardley, D. D., 1983, Immunochemical characterization of the mucoid exopolysaccharide of Pseudomonas aeruginosa,J. Infect. Dis. 147:494–503.

    Article  PubMed  CAS  Google Scholar 

  68. Daley, L., Pier, G. B., Liporace, J. D., and Eardley, D. D., 1985, Polyclonal B cell stimulation and interleukin 1 induction by the mucoid exopolysaccharide of Pseudomonas aeruginosa associated with cystic fibrosis, J. Immunol. 134:3089–3093.

    PubMed  CAS  Google Scholar 

  69. Irvin, R. T., and Ceri, H., 1985, Immunochemical examination of the Pseudomonas aeruginosa glycocalyx: A monoclonal antibody which recognizes L-guluronic acid residues of alginic acid, Can. J. Microbiol. 31:268–275.

    Article  PubMed  CAS  Google Scholar 

  70. Ceri, H., McArthur, H. A. I., and Whitfield, C., 1986, Association of alginate from Pseudomonas aeruginosa with two forms of heparin-binding lectin isolated from rat lung, Infect. Immun. 51:1–5.

    PubMed  CAS  Google Scholar 

  71. Baker, N. R., and Marcus, H., 1982, Adherence of clinical isolates of Pseudomonas aeruginosa to hamster tracheal epithelium in vitro, Curr. Microbiol. 7:35–40.

    Article  Google Scholar 

  72. Baker, N. R., and Tao, Y., 1982, A tracheal culture model of respiratory tract infection with Pseudomonas aeruginosa, In Vitro,18:369–376.

    Article  PubMed  CAS  Google Scholar 

  73. Ramphal, R., and Pyle, M., 1983, Adherence of mucoid and nonmucoid Pseudomonas aeruginosa to acid-injured tracheal epithelium, Infect. Immun. 41:345–351.

    PubMed  CAS  Google Scholar 

  74. Marcus, H., and Baker, N. R., 1985, Quantitation of adherence of mucoid and nonmucoid Pseudomonas aeruginosa to hamster tracheal epithelium, Infect. Immun. 47:723–729.

    PubMed  CAS  Google Scholar 

  75. Woods, D. E., and Sokol, P. A., 1985, Use of transposon mutants to assess the role of exoenzyme S in chronic pulmonary disease due to Pseudomonas aeruginosa, Eur. J. Clin. Microbiol. 4:163–169.

    Article  PubMed  CAS  Google Scholar 

  76. Woods, D. E., To, M., and Sokol, P. A., 1989, Pseudomonas aeruginosa exoenzyme S as a pathogenic determinant in respiratory tract infections, Antibiot. Chemother. 42:27–35.

    CAS  Google Scholar 

  77. Coburn, J., Yatt, R. T., Iglewski, B. H., and Gill, D. M., 1989, Several GTP-binding proteins, including p21, h.ras, are preferred substrates of Pseudomonas aeruginosa exoenzyme S, J. Biol. Chem. 264:9004–9008.

    PubMed  CAS  Google Scholar 

  78. Woods, D. E., and Que, J. U., 1987, Purification of Pseudomonas aeruginosa exoenzyme S, Infect. Immun. 55:579–586.

    PubMed  CAS  Google Scholar 

  79. Lingwood, C. A., Cheng, M., Krivan, H. C., and Woods, D. E., 1991, Glycolipid receptor binding specificity of exoenzyme S from Pseudomonas aeruginosa, Biochem. Biophys. Res. Comm. 175:1076–1081.

    Article  PubMed  CAS  Google Scholar 

  80. Frost, L. S., Carpenter, M., and Paranchych, W, 1978, N-methyphenylalaline at the N-terminus of pilin isolated from Pseudomonas aeruginosa K, Nature 271:87–89.

    Article  PubMed  CAS  Google Scholar 

  81. Elleman, T. C., Hoyne, P. A., Stewart, D. J., McKern, N. M., and Peterson, J. E., 1986, Expression of pili from Bacteroides nodosus in Pseudomonas aeruginosa, J. Bacteriol. 168:574–580.

    PubMed  CAS  Google Scholar 

  82. Elleman, T. C., and Peterson, J. E., 1987, Expression of multiple types of N-methyl Phe pili in Pseudomonas aeruginosa, Mol. Microbiol. 1:377–380.

    Article  PubMed  CAS  Google Scholar 

  83. Paranchych, W, and Frost, L. S., 1988, The physiology and biochemistry of pili, Adv. Microbiol. Phys. 29:53–114.

    Article  CAS  Google Scholar 

  84. Beard, M. K. M., Mattick, J. S., Moore, L. J., Mott, M. R., Marrs, C. E., and Egerton, J. R., 1990, Morphogenetic expression of Moraxella bovis fimbriae (pili) in Pseudomonas aeruginosa, J. Bacteriol. 172:2601–2607.

    PubMed  CAS  Google Scholar 

  85. Ottow, J. C. G., 1975, Ecology, physiology, and genetics of fimbriae and pili, Ann. Rev. Microbiol. 29:79–108.

    Article  CAS  Google Scholar 

  86. Folkhard, W, Leonard, K. R., Malsey, S., Marvin, D. A., Dubochet, J., Engel, A., Achtman, M., and Helmuth, R., 1979, X-ray diffraction and electron microscopic studies on the structure of bacterial F-pili, J. Mol. Biol. 130:145–160.

    Article  PubMed  CAS  Google Scholar 

  87. Folkhard, W, Marvin, D.A., Watts, T. H., and Paranchych, W, 1981, Structure of polar pili from Pseudomonas aeruginosa strains K and O, J. Mol. Biol. 149:79–93.

    Article  PubMed  CAS  Google Scholar 

  88. Marvin, D. A., and Folkhard, W, 1986, Structure of F-pili: Reassessment of the symmetry. J. Mol. Biol. 191:299–300.

    Article  PubMed  CAS  Google Scholar 

  89. Frost, L. S., and Paranchych, W, 1977, Composition and molecular weight of pili purified from Pseudomonas aeruginosa K, J. Bacteriol. 131:259–269.

    PubMed  CAS  Google Scholar 

  90. Paranchych, W, Sastry, R A., Frost, L. S., Carpenter, M., Armstrong, G. D., and Watts, T. H. 1979, Biochemical studies on pili isolated from Pseudomonas aeruginosa strain PAO, Can. J. Microbiol. 26:1175–1181.

    Article  Google Scholar 

  91. Pasloske, B. L., Finlay, B. B., and Paranchych, W, 1985, Cloning and sequencing of Pseudomonas aeruginosa PAK pilin gene, FEBS Lett. 183:408–412.

    Article  PubMed  CAS  Google Scholar 

  92. Sastry, P. A., Finlay, B. B., Pasloske, B. L., Paranchych, W, Pearlstone, J. R., and Smillie, L. B., 1985, Comparative studies on the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO, J. Bacteriol. 164:571–577.

    CAS  Google Scholar 

  93. Sastry, P. A., Pearlstone, J. R., Smillie, L. B., and Paranchych, W, 1985, Studies on the primary structure and antigenic determinants of pilin isolated from Pseudomonas aeruginosa K, Can. J. Biochem. Cell Biol. 63:284–291.

    Article  PubMed  CAS  Google Scholar 

  94. Johnson, K., Parker, M. L., and Lory, S., 1986, Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes, J. Biol. Chem. 261:15703–15708.

    PubMed  CAS  Google Scholar 

  95. Pasloske, B. L., Sastry, P A., Finlay, B. B., and Paranchych, W, 1988, Two unusual pilin sequences from different isolates of Pseudomonas aeruginosa, J. Bacteriol. 170:3738–3741.

    PubMed  CAS  Google Scholar 

  96. Finlay, B. B., Pasloske, B. L., and Paranchych, W, 1986, Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli, J. Bacteriol. 165:625–630.

    PubMed  CAS  Google Scholar 

  97. Strom, M. S., and Lory, S., 1986, Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli, J. Bacteriol. 167:367–372.

    Google Scholar 

  98. Johnson, K., and Lory, S., 1987, Characterization of Pseudomonas aeruginosa mutants with altered piliation, J. Bacteriol. 169:5663–5667.

    PubMed  CAS  Google Scholar 

  99. Strom, M. S., and Lory, S., 1987, Mapping of export signals of Pseudomonas aeruginosa pilin with alkaline phosphatase fusions, J. Bacteriol. 169:3181–3188.

    PubMed  CAS  Google Scholar 

  100. Pasloske, B. L., and Paranchych, W, 1988, The expression of mutant pilins in Pseudomonas aeruginosa: Fifth position glutamate affects pilin methylation, Mol. Microbiol. 2:489–495.

    Article  PubMed  CAS  Google Scholar 

  101. Nunn, D., Bergman, S., and Lory, S., 1990, Products of three accessory genes, pila, piiC,and pilD, are required for biogenesis of Pseudomonas aeruginosa pili, J. Bacteriol. 172:2911–2919.

    PubMed  CAS  Google Scholar 

  102. Watts, T. H., Sastry, P. A., Hodges, R. S., and Paranchych, W, 1983, Mapping of antigenic determinants of Pseudomonas aeruginosa PAK polar pili, Infect. Immun. 42:113–121.

    PubMed  CAS  Google Scholar 

  103. Dalrymple, B., and Mattick, J. S., 1987, An analysis of the organization and evolution of the type 4 fimbrial (MePhe) subunit proteins, J. Mol. Evol. 25:261–269.

    Article  PubMed  CAS  Google Scholar 

  104. Watts, T. H., Worobec, E. A., and Paranchych, W, 1982, Identification of pilin pools in the membranes of Pseudomonas aeruginosa, J. Bacteriol. 152:687–691.

    PubMed  CAS  Google Scholar 

  105. Leow, M. A., Holt, S. C., and Eisentein, B. I., 1987, Immunoelectron microscopic analysis of elongation of type 1 fimbriae in Escherichia coli, J. Bacteriol. 169:157–163.

    Google Scholar 

  106. Lund, B., Lindberg, E, Marklund, B.-I., and Normark, S., 1987, The PapG protein is the a-D-galactopyranosyl-(1–4)-ß-d galactopyranose-binding adhesin of uropathogenic Escherichia coli, Proc. Natl. Acad. Sci. USA 84:5898–5902.

    Article  PubMed  CAS  Google Scholar 

  107. Worobec, E. A., Frost, L. S., Pieroni, P., Armstrong, G. D., Hodges, R. S., Parker, J. M. R., Finlay, B. B., and Paranchych, W, 1986, Location of the antigenic determinants of conjunctive F-like pill, J. Bacterial. 167:660–665.

    CAS  Google Scholar 

  108. Bradley, D. E., 1972, A study of pili on Pseudomonas aeruginosa, Genet. Res. 19:39–51.

    Article  Google Scholar 

  109. Bradley, D. E., 1974, The adsorption of Pseudomonas aeruginosa pilus-dependent bacteriophages to a host mutant with nonretractile pili, Virology 58:149–163.

    Article  PubMed  CAS  Google Scholar 

  110. Bradley, D. E., and Pitt, T. L., 1974, Pilus-dependence of four Pseudomonas aeruginosa bacteriophages with non-contractile tails, J. Gen. Virol. 24:1–15.

    Article  PubMed  CAS  Google Scholar 

  111. Bradley, D. E., 1980, A function of Pseudomonas aeruginosa PAO polar pili: Twitching motility, Can. J. Microbial. 26:146–154.

    Article  CAS  Google Scholar 

  112. Fulks, K.A., Marrs, C. E, Stevens, S. P., and Green, M. R., 1990, Sequence analysis of the inversion region containing the pilin genes of Moraxella bovis, J.Bacteriol. 172:310–316.

    PubMed  CAS  Google Scholar 

  113. De Ree, J. M., Schwillens, P., and van den Boscht, J. E., 1987, Monoclonal antibodies raised against Pap fimbriae recognize minor component(s) involved in receptor binding, Microb. Pathogen. 2:113–121.

    Article  Google Scholar 

  114. Lund, B., Lindberg, E, and Normark, S., 1988, Structure and antigenic properties of the tip-located P pilus proteins of uropathogenic Escherichia coli,J. Bacteriol. 170:1887–1894.

    PubMed  CAS  Google Scholar 

  115. Mirelman, D., and Ofek, I., 1986, Introduction to microbial lectins and agglutinins. In: Microbial Lectins and Agglutinins: Properties and Biological Activity (D. Mirelman, ed.), John Wiley & Sons, Inc., New York, pp. 1–19.

    Google Scholar 

  116. Abraham, S. N., Goguen, J. D., Sun, D., Klemm, R and Beachey, E. H., 1987, Identification of two ancillary subunits of Escherichia coli type 1 fimbriae by using antibodies against synthetic oligopeptides of fim gene products, J. Bacteriol. 169:5530–5536.

    PubMed  CAS  Google Scholar 

  117. Hanson, M. S., Hempel, J., and Brinton, C. C., Jr., 1988, Purification of the Escherichia coli type 1 pilin and minor pilus proteins and partial characterization of the adhesin protein, J. Bacteriol. 170:3350–3358.

    PubMed  CAS  Google Scholar 

  118. Mattick, J. S., Bills, M. M., Anderson, B. J., Dalrymple, B., Mott, M. R., and Egerton, J. R., 1987, Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa, J. Bacteriol. 169:33–41.

    PubMed  CAS  Google Scholar 

  119. Woods, D. E., Bass, J. A., Johanson, W.G., Jr., and Straus, D.C., 1980, Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients, Infect. Immun. 30:694–699.

    PubMed  CAS  Google Scholar 

  120. Woods, D. E., Straus, D. C., Johanson, W. G., Jr., and Bass, J. A., 1981, Role of salivary protease activity in adherence of gram-negative bacilli to mammalian buccal epithelial cells in vivo, J. Clin. Invest. 68: 1435–1440.

    Article  PubMed  CAS  Google Scholar 

  121. . Woods, D.E., Straus, D.C., Johanson, W.G., Jr., and Bass, J.A., 1983, Factors influencing the adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells, Rev. Infect. Dis. 5:S847—S851.

    Google Scholar 

  122. Ramphal, R., Small, P. M., Shands, J. W., Jr., Fischlschweiger, W, and Small, P. A., Jr., 1980, Adherence of Pseudomonas aeruginosa to tracheal cells injured by influenza infection or by endotracheal intubation, Infect. Immun. 27:614–619.

    PubMed  CAS  Google Scholar 

  123. Sato, H., Okinaga, K., and Saito, H., 1988, Role of pili in the pathogenesis of Pseudomonas aeruginosa burn infection, Microbiol. Immunol. 32:131–139.

    PubMed  CAS  Google Scholar 

  124. Irvin, R. T., Doig, P., Lee, K. K., Sastry, P. A., Paranchych, W, Todd, T., and Hodges, R. S., 1989, Characterization of the Pseudomonas aeruginosa pilus adhesin: Confirmation that the pilin structural protein subunit contains a human epithelial cell binding domain, Infect. Immun. 57:3720–3726.

    PubMed  CAS  Google Scholar 

  125. Lee, K. K., Doig, P, Irvin, R. T., Paranchych, W, and Hodges, R. S., 1989, Mapping the surface regions of Pseudomonas aeruginosa PAK pilin: The importance of the C-terminal region for adherence to human buccal epithelial cells, Molec. Microbiol. 3:1493–1499.

    Article  CAS  Google Scholar 

  126. Schmidt, M. A., and O’Hanley, P., 1988, Synthetic peptides corresponding to protective epitopes of Escherichia coli digalactoside-binding pilin prevent infection in a murine pyelonephritis model, Proc. Natl. Acad. Sci. USA 85:1247–1251.

    Article  PubMed  CAS  Google Scholar 

  127. Ramphal, R., and Pyle, M., 1985, Further characterization of the tracheal receptor for Pseudomonas aeruginosa, Eur. J. Clin. Microbiol. 4:160–162.

    Article  PubMed  CAS  Google Scholar 

  128. Vishwanath, S., and Ramphal, R., 1985, Adherence of Pseudomonas aeruginosa to human tracheobronchial mucin, Infect. Immun. 45:197–202.

    Google Scholar 

  129. Ramphal, R., Carnoy, C., Fievre, S., Michalski, J.-C., Houdret, N., Lamblin, G., Strecker, G., and Roussel, P., 1991, Pseudomonas aeruginosa recognizes carbohydrate chains containing type 1 (Gal(31–3G1cNAc) or type 2 (Gal(31–4G1cNAc) disaccharide units, Infect. Immun. 59: 700–704.

    CAS  Google Scholar 

  130. Doig, P, Paranchych, W, Sastry, P. A., and Irvin, R. T., 1989, Human buccal epithelial cell receptors of Pseudomonas aeruginosa: Identification of glycoproteins with pilus binding activity, Can. J. Microbiol. 35:1141–1145.

    Article  PubMed  CAS  Google Scholar 

  131. Krivan, H. C., Roberts, D. D., and Ginsburg, V, 1988, Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence Ga1NA11–4Gal found in some glycolipids, Proc. Natl. Acad. Sci. USA 85:6157–6161.

    Article  PubMed  CAS  Google Scholar 

  132. Stromberg, N., Deal, C., Nyberg, G., Normark, S., So, M., and Karlsson, K.-A., ‘888, Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae, Proc. Natl. Acad. Sci. USA 85:4902–4906.

    Google Scholar 

  133. Karlsson, K.-A., 1989, Animal glycosphingolipids as membrane attachment sites for bacteria, Ann. Rev. Biochem. 58:309–350.

    Article  PubMed  CAS  Google Scholar 

  134. Jimenez-Lucho, V., Ginsburg, V, and Krivan, H. C., 1990, Cryptococcus neoformansCandida albicans, and other fungi bind specifically to the glycosphingolipid lactosylceramide (Galß1–4Glc(31–1Cer), a possible adhesion receptor for yeasts, Infect. Immun. 58:2085–2090.

    PubMed  CAS  Google Scholar 

  135. Nyberg, G., Stromberg, N., Johnsson, A., Karlsson, K.-A., and Normark, S., 1990, Erythrocyte gangliosides act as receptors for Neisseria subflava: Identification of the Sia-1 adhesin, Infect. Immun. 58:2555–2563.

    PubMed  CAS  Google Scholar 

  136. Wong, W. Y., Irvin, R. T., Paranchych, W, and Hodges, R. S., 1991, Antigen-antibody interactions: Elucidation of the epitope and strain-specificity of a monoclonal antibody directed against the pilin adherence domain of Pseudomonas aeruginosa strain K, submitted.

    Google Scholar 

  137. Sajjan, U., Reisman, J., Doig, P., Irvin, R. T., Forstner, G., and Forstner, J., 1991, Interaction of nonmucoid Pseudomonas aeruginosa with normal human intestinal mucin and respiratory mucin from patients with cystic fibrosis, J. Clin. Invest., in press.

    Google Scholar 

  138. Irvin, R. T., 1990, Hydrophobicity of proteins and bacterial fimbriae. In: Microbial Cell Surface Hydrophobicity (Doyle, R. J., and Rosenberg, M., eds.), American Society for Microbiology, Washington, D.C., pp. 137–178.

    Google Scholar 

  139. Garner, C. V., DesJardins, D., and Pier, G. B., 1990, Immunogenic properties of Pseudomonas aeruginosa mucoid exopolysaccharide, Infect. Immun. 58:1835–1842.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7

    Randall T. Irvin

Authors
  1. Randall T. Irvin
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Pisa, Pisa, Italy

    Mario Campa  & Mauro Bendinelli  & 

  2. University of South Florida, Tampa, Florida, USA

    Herman Friedman

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer Science+Business Media New York

About this chapter

Cite this chapter

Irvin, R.T. (1993). Attachment and Colonization of Pseudomonas aeruginosa: Role of the Surface Structures. In: Campa, M., Bendinelli, M., Friedman, H. (eds) Pseudomonas aeruginosa as an Opportunistic Pathogen. Infectious Agents and Pathogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3036-7_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-3036-7_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6324-8

  • Online ISBN: 978-1-4615-3036-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation