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Genetic Regulation of the Murine Corneal and Non-Corneal Response to Pseudomonas aeruginosa

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Pseudomonas aeruginosa as an Opportunistic Pathogen

Part of the book series: Infectious Agents and Pathogenesis ((IAPA))

Abstract

During the past several years an active interest has developed in genetic regulation of host susceptibility and resistance to both infection and malignancy.1 The potential role of genetic makeup of individuals as a major determinant of susceptibility to a variety of infectious agents receives strong support from epidemiologic data. Concomitantly, the widespread development of genetically defined strains of mutant, congenic, and recombinant inbred mice have made it possible for investigators to make new advances in this field. According to Skamene,l this allows for detailed mapping of the murine genome, resulting in the identification of the various chromosomal loci controlling resistance and susceptibility to a given infectious or oncogenic stimulus. This strategy has already resulted in the identification of several chromosomal loci involved in regulation of various traits. As Skamene points out, resistance genes to various infectious or malignant agents appear to map to the same locus1. This suggests that there may be common mechanisms of host resistance to combat a variety of unrelated agents. Once these various genes have been identified, we can then begin to investigate the various cellular and molecular mechanisms that underlie the traits of resistance and susceptibility, thereby developing a better understanding of host defense mechanisms along with the potential for developing remedial measures.

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References

  1. Berk, R. S., Hazlett, L. D., Potter, M., and Beisel, K. W, 1985, Multigenic regulation of natural resistance of the mouse cornea to Pseudomonas aeruginosa,in: Genetic Control of Host Resistance to Infection and Malignancy, Progress in Leukocyte Biology, Volume III (E. Skamene, ed.), Alan R. Liss, New York, pp. 367–372.

    Google Scholar 

  2. Biozzi, G., Mouton, D., Siqueira, M., and Stiffel, C., 1985, Effect of genetic modification of immune responsiveness on anti-infection and anti-tumor resistance, in: Genetic Control of Host Resistance to Infection and Malignancy, Progress in Leukocyte Biology, Volume III (E. Skamene, ed.), Alan R. Liss, New York, pp. 13–18.

    Google Scholar 

  3. McPherson, M. A., and Dormer, R. L., 1987, The molecular and biochemical basis of cystic fibrosis, Biosci. Rep. 7:167–185.

    Article  PubMed  CAS  Google Scholar 

  4. McPherson, M. A., and Goodchild, M. C., 1988, The biochemical defect in cystic fibrosis, Clin. Sci. 74:337–345.

    PubMed  CAS  Google Scholar 

  5. Luzar, M. A., Thomassen, M. J., and Montie, T. C., 1985, Flagella and motility alterations in Pseudomonas aeruginosa strains from patients with cystic fibrosis: Relationship to patient clinical condition, Infect. Immun. 50:577–582.

    PubMed  CAS  Google Scholar 

  6. Pier, G. B., 1986, Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: Current status of the host-bacterium interaction, J. Infect. Dis. 151:575–580.

    Article  Google Scholar 

  7. Houdret, N., Ramphal, R., Scharfman, A., Perini, J. M, Filliat, G., Lamblin, G., and Roussel, P., 1989, Evidence for the in vivo degradation of human respiratory mucins during Pseudomonas aeruginosa infection, Biochem. Biophys. Acta. 992:96–105.

    Article  PubMed  CAS  Google Scholar 

  8. Nelson, J. W, Tredgett, M. W, Sheehan, J. K., Thornton, D. J., Notman, D., and Govan, J. R. W., 1990, Mucinophilic and chemotactic properties of Pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis, Infect. Immun. 58:1489–1495.

    PubMed  CAS  Google Scholar 

  9. Pennington, J. E., and Williams, R. M., 1979, Influence of genetic factors on natural resistance of mice to Pseudomonas aeruginosa,J. Infect. Dis. 139:396–400.

    Article  PubMed  CAS  Google Scholar 

  10. Pier, G. B., 1982, Cross-protection by Pseudomonas aeruginosa polysaccharides, Infect. Immun. 38:1117–1122.

    PubMed  CAS  Google Scholar 

  11. Markham, R. B., and Pier, G. B., 1983, Characterization of the antibody response in inbred mice to high-molecular-weight polysaccharide from Pseudomonas aeruginosa immunotype 1, Infect. Immun. 41:232–236.

    PubMed  CAS  Google Scholar 

  12. Pier, G. B., and Markham, R. B., 1982, Induction in mice of cell-mediated immunity to Pseudomonas aeruginosa by high molecular weight polysaccharide and vinblastine, J. Immunol. 128:2121–2125.

    PubMed  CAS  Google Scholar 

  13. Markham, R. B., Goellner, J. B., and Pier, G. B., 1984, In vitro T cell-mediated killing of Pseudomonas aeruginosa. I. Evidence that a lymphokine mediates killing, J. Immunol. 133: 962–968.

    PubMed  CAS  Google Scholar 

  14. Markham, R. B., Pier, G. B., Goellner, J. Y., and Mizel, S. B., 1985, In vitro T-cell mediated killing of Pseudomonas aeruginosa. II. The role of macrophages and T cell subsets in T cell killing, J. Immunol. 134:4112–4117.

    PubMed  CAS  Google Scholar 

  15. Powderly, W. G., Pier, G. B., and Markham, R. B., 1986, T lymphocyte-mediated protection against Pseudomonas aeruginosa in granulocytopenic mice. J. Clin. Invest. 78:375–380.

    Article  PubMed  CAS  Google Scholar 

  16. Powderly, W. G., Pier, G. B., and Markham, R. B., 1986, In vitro T cell-mediated killing of Pseudomonas aeruginosa. III. The role of suppressor T cells in nonresponder mice, J. Immunol. 136:299–303.

    PubMed  CAS  Google Scholar 

  17. Sherman, L. A., 1982, Genetic linkage of the cytolytic T lymphocyte repertoire and immunoglobulin heavy chain genes, J. Exp. Med. 156:294–299.

    Article  PubMed  CAS  Google Scholar 

  18. Sherman, L. A., and Riblet, R., 1985, Comparison of the H-2Kb-specific cytolytic T lymphocyte receptor repertoire in Igh recombinant strains, J. Immunol. 134:3569–3573.

    PubMed  CAS  Google Scholar 

  19. Owen, E. L., Riblet, R., and Taylor, B. A., 1981, The T suppressor cell alloantigen Tsud maps near immunoglobulin allotype genes and may be a heavy chain constant-region marker on a T cell receptor, J. Exp. Med. 153:801–810.

    Article  PubMed  CAS  Google Scholar 

  20. Owen, F. L, and Riblet, R., 1984, Genes for the mouse T cell alloantigens Tpre, Tthy, Tind, and Tsu are closely linked near Igh on chromosome 12, J. Exp. Med. 159:313–317.

    Article  PubMed  CAS  Google Scholar 

  21. Powderly, W. G., Pier, G. B., and Markham, R. B., 1986, In vitro T cell-mediated killing of Pseudomonas aeruginosa. IV. Nonresponsiveness in polysaccharide-immunized BALB/c mice is attributable to vinblastine-sensitive suppressor cells, J. Immunol. 137:2025–2030.

    PubMed  CAS  Google Scholar 

  22. Powderly, W. G., Pier, G. B., and Markham, R. B., 1987, In vitro T cell-mediated killing of Pseudomonas aeruginosa. V. Generation of bactericidal T cells in nonresponder cells, J. Immunol. 138:2272–2277.

    PubMed  CAS  Google Scholar 

  23. Markham, R. B., and Powderly, W. G., 1988, Exposure of mice to live Pseudomonas aeruginosa generates protective cell-mediated immunity in the absence of an antibody response, J. Immunol. 141:2039–2045.

    Google Scholar 

  24. Markham, R. B., Pier, G. B., and Powderly, W, 1988, Suppressor T cells regulating the cell-mediated immune response to Pseudomonas aeruginosa can be generated by immunization with anti-bacterial T cells, J. Immunol. 141:3975–3979.

    PubMed  CAS  Google Scholar 

  25. Burns, R. P., and Rhodes, D. H., Jr., 1961, Pseudomonas eye infection as a cause of death in premature infants, Arch. Opthalmol. 65:517–525.

    Article  CAS  Google Scholar 

  26. Hazlett, L. D., Zelt, R., Cramer, C., and Berk, R. S., 1985, Pseudomonas aeruginosa induced ocular infection: A histopathological comparison of two strains of different virulence. Ophthalmic Res. 17:289–296.

    Article  PubMed  CAS  Google Scholar 

  27. Twining, S. S., Lohr, K. M., and Moulder, J. E., 1986, The immune system in experimental Pseudomonas keratitis. Model and early effects, Invest. Ophthalmol. Vis. Sci. 27:507–515.

    PubMed  CAS  Google Scholar 

  28. Van Horn, D. L., Davis, S. D., Hyndiuk, R. A., and Pedersen, H. J., 1981, Experimental Pseudomonas keratitis in the rabbit: Bacteriological, clinical and microscopic observation, Invest. Ophthalmol. Vis. Sci. 20:213–221.

    PubMed  Google Scholar 

  29. Hazlett, L. D., Rosen, D., and Berk, R. S., 1976, Experimental eye infections caused by Pseudomonas aeruginosa, Ophthalmic Res. 8:311–318.

    Article  Google Scholar 

  30. Berk, R. S., Leon, M. A., and Hazlett, L. D., 1979, Genetic control of the murine corneal response to Pseudomonas aeruginosa, Infect. Immun. 26:1221–1223.

    PubMed  CAS  Google Scholar 

  31. Berk, R. S., Beisel, K. W, and Hazlett, L. D., 1981, Genetic studies of the murine corneal response to Pseudomonas aeruginosa, Infect. Immun. 34:1–5.

    PubMed  CAS  Google Scholar 

  32. Beisel, K. W, Hazlett, L. D., and Berk, R. S., 1983, Genetic studies on a dominant susceptibility effect on the murine corneal response to Pseudomonas aeruginosa, Proc. Soc. Exp. Biol. Med. 172:488–491.

    PubMed  CAS  Google Scholar 

  33. Berk, R. S., Hazlett, L. D., Potter, M., and Beisel, K. W, 1985, Multigenic regulation of natural resistance of the mouse cornea to Pseudomonas aeruginosa, in: Genetic Control of Host Resistance to Infection and Malignancy, Volume III (E. Skamene, ed.), Allan R. Liss, Inc., New York, pp. 367–372.

    Google Scholar 

  34. Berk, R. S., Hazlett, L. D., and Beisel, K. W, 1987, Genetic studies on resistant and susceptibility genes controlling the mouse cornea to infection with Pseudomonas aeruginosa,in Antibiotics and Chemotherapy. Basic Research and Clinical Aspects of Pseudomonas aeruginosa, Volume 39 (G. Döring, I. A. Holder, and K. Botzenhart, eds.), Karger, Basel, pp. 83–91.

    Google Scholar 

  35. Berk, R. S., Hazlett, L. D., and Beisel, K. W, 1989, Location of genes regulating resistance of the mouse cornea to Pseudomonas aeruginosa infection, in Modern Trends in Immunology and Immunopathology of the Eye (A. G. Secchi and I. A. Fregoni, eds.), Masson, Milan, pp. 123–127.

    Google Scholar 

  36. Hazlett, L. D., and Berk, R. S., 1978, Heightened resistance of athymic nude (nu/nu) mice to experimental Pseudomonas aeruginosa ocular infection, Infect. Immun. 22:926–933.

    PubMed  CAS  Google Scholar 

  37. Hirst, R. G., and Wallace, M. E., 1976, Inherited resistance to Corynebacterium kutscheri in mice, Infect. Immun. 14:475–482.

    PubMed  CAS  Google Scholar 

  38. Pierce-Chase, C. H., Fauve, R. M., and Dubos, R., 1964, Corynebacterial pseudotuberculosis in mice. I. Comparative susceptibility of mouse strains to experimental infection with Corynebacterium kutscheri,J. Exp. Med. 120:267–284.

    Article  PubMed  CAS  Google Scholar 

  39. Closs, O., and Haugen, O. A., 1973, Experimental murine leprosy. I. Clinical and histological evidence for varying susceptibility of mice to infection with Mycobacterium lepraemurium, Acta Patted. Microbiol. Scand. Sect. A, 81:401–410.

    CAS  Google Scholar 

  40. Gloss, O., and Haugen, O. A., 1974, Experimental murine leprosy. 2. Further evidence for varying susceptibility of outbred mice and evaluation of the response of 5 inbred mouse strains to infection with Mycobacterium lepraemurium, Acta Pathol. Microbiol. Scand. Sect. A, 82: 459–474.

    Google Scholar 

  41. Allen, E. M., Moore, V. L., and Stevens, J. O., 1977, Strain variation in BCG-induced chronic pulmonary inflammation in mice. I. Basic model and possible genetic control by non-H-2 genes, J. Immunol. 119:343–347.

    PubMed  CAS  Google Scholar 

  42. Cheers, C., and McKenzie, I. E C., 1978, Resistance and susceptibility of mice to bacterial infection: Genetics of listeriosis, Infect. Immun. 19:755–762.

    PubMed  CAS  Google Scholar 

  43. Cheers, C., McKenzie, I. F. C., Pavlov, H., Waid, C., and York, J., 1978, Resistance and susceptibility of mice to bacterial infection: Course of listeriosis in resistant or susceptible mice, Infect. Immun. 19:763–770.

    PubMed  CAS  Google Scholar 

  44. Farr, A. G., Kiely, J.-J., and Unanue, E. R., 1979, Macrophage -T cell interactions involving Listeria monocytogenes-Role of the H-2 gene complex, J. Immunol. 122:2395–2404.

    PubMed  CAS  Google Scholar 

  45. Skamene, E., Kongshavn, P. A. I.., and Sachs, D. H., 1979, Resistance to Listeria monocytogenes in mice: Genetic control by genes that are not linked to the H-2 complex, J. Infect. Dis. 139: 228–231.

    Article  PubMed  CAS  Google Scholar 

  46. Groves, M. G., and Osterman, J. V., 1978, Host defenses in experimental scrub typhus: Genetics of natural resistance to infection, Infect. Immun. 19:583–588.

    PubMed  CAS  Google Scholar 

  47. Anderson, Jr., G. W, and Osterman, J. V., 1980, Host defenses in experimental rickettsialpox: Genetics of natural resistance to infection, Infect. Immun. 28:132–136.

    PubMed  Google Scholar 

  48. Gowen, J. W, and Calhoun, M. L., 1943, Factors affecting genetic resistance of mice to mouse typhoid, J. Infect. Dis. 73:40–56.

    Article  Google Scholar 

  49. Plant, J., and Glynn, A. A., 1974, Natural resistance to Salmonella infection, delayed hypersensitivity and Ir genes in different strains of mice, Nature 248:345–347.

    Article  PubMed  CAS  Google Scholar 

  50. Plant, J., and Glynn, A. A., 1976, Genetics of resistance to infection with Salmonella typhimurium in mice, J. Infect. Dis. 133:72–78.

    Article  PubMed  CAS  Google Scholar 

  51. Robson, H. G., and Vas, S. I., 1972, Resistance of inbred mice to Salmonella typhimurium,J. Infect. Dis. 126:378–386.

    Article  PubMed  CAS  Google Scholar 

  52. Lissner, C. R., Swanson, R. N., and O’Brien, A. D., 1983, Genetic control of the innate resistance of mice to Salmonella typhimurium: Expression of the Ity gene in peritoneal and splenic macrophages isolated in vitro, J. Immunol. 131:3006–3013.

    PubMed  CAS  Google Scholar 

  53. Bradley, D. H., Taylor, B. A., Blackwell, J., Evans, E. P., and Freeman, J., 1979, Regulation of leishmania populations within the host. III. Mapping of the locus controlling susceptibility to visceral leishmaniasis in the mouse, Clin. Exp. Immunol. 37:7–14.

    PubMed  CAS  Google Scholar 

  54. Brown, I. N., Glynn, A. A., and Plant, J., 1982, Inbred mouse strain resistance to Mycobacterium lepraemurium follows Ity/Lsh pattern, Immunol. 47:149–156.

    CAS  Google Scholar 

  55. Skamene, E., Gros, P, Forget, A., Kongshavn, P. A. L., St. Charles, C., and Taylor, B. A., 1982, Genetic regulation of resistance to intracellular pathogens, Nature 297:506–509.

    Article  PubMed  CAS  Google Scholar 

  56. Berk, R. S., Montgomery, I. N., and Hazlett, L. D., 1988, Serum antibody and ocular responses to murine corneal infection caused by Pseudomonas aeruginosa, Infect. Immun. 56: 3076–3080.

    PubMed  CAS  Google Scholar 

  57. Cleveland, R. P., Hazlett, L. D., Leon, M. A., and Berk, R. S., 1983, The role of complement in Pseudomonasocular infections, Incest. Ophthalmol. Vis. Sci. 24:237–242.

    CAS  Google Scholar 

  58. Nilsson, U. R., and Muller-Eberhard, H. J., 1967, Deficiency of the fifth component of complement in mice with an inherited complement defect, J. Exp. Med. 125:1–16.

    Article  PubMed  CAS  Google Scholar 

  59. Newman, K., Jr., and Johnson, R. C., 1981, In vivo evidence that an intact lytic complement pathway is not essential for successful removal of circulating Borrelia turicatae from mouse blood, Infect. Immun. 31:465–469.

    PubMed  Google Scholar 

  60. Mondino, B. J., Ratajczak, H. V., Goldberg, D. B., Schanzlin, D. J., and Brown, S. I., 1980, Alternate and classical pathway components of complement in the normal cornea, Arch. Ophthalmol. 98:346–349.

    Article  PubMed  CAS  Google Scholar 

  61. Mondino, B. J., and Hoffman, D. B., 1980, Hemolytic complement activity in normal human donor corneas, Arch. Ophthalmol. 98:2041–2044.

    Article  PubMed  CAS  Google Scholar 

  62. Mondino, B. J., Brown, S. I., Rabin, B. S., and Bruno, J., 1978, Alternate pathway activation of complement in a Proteus mirabilis ulceration of the cornea, Arch. Ophthalmol. 96:1659–1661.

    Article  PubMed  CAS  Google Scholar 

  63. Hazlett, L. D., Rosen, D. D., and Berk, R. S., 1978, Age-related susceptibility to Pseudomonas aeruginosa ocular infections in mice, Infect. Immun. 20:25–29.

    PubMed  CAS  Google Scholar 

  64. Berk, R. S., Iglewski, B. H., and Hazlett, L. D., 1981, Age-related susceptibility of mice to ocular challenge with Pseudomonas aeruginosa exotoxin A,Infect. Immun. 33:90–94.

    PubMed  CAS  Google Scholar 

  65. Kreger, A. S., Lyerly, D. M., Hazlett, L. D., and Berk, R. S., 1986, Immunization against experimental Pseudomonas aeruginosa and Serratia marcescens keratitis: Vaccination with lipopolysaccharide endotoxins and proteases, Invest. Ophthalmol. Vis. Sci. 27:932–939.

    PubMed  CAS  Google Scholar 

  66. Moon, M. M., Hazlett, L. D., Hancock, R. E. W, Berk, R. S., and Barrett, R., 1988, Monoclonal antibodies provide protection against ocular Pseudomonas aeruginosainfection, Invest. Ophthalmol. Vis. Sci. 29:1277–1284.

    PubMed  CAS  Google Scholar 

  67. McMeel, J. W, and Wood, R. M., 1960, Passive immunization against pseudomonas infection of the cornea, Trans. Am. Acad. Ophthalmol. Otolarnygol. 64:486–489.

    Google Scholar 

  68. Young, L. S., 1974, Role of antibody in infections due toPseudomonas aeruginosa, J. Infect. Dis. 130:S111—S118.

    PubMed  Google Scholar 

  69. Cryz, S. J., Jr., Furer, E., and Germanier, R., 1983, Protection against Pseudomonas aeruginosa infection in a murine burn wound sepsis model by passive transfer of antitoxin A, antielastase, and antilipopolysaccharide, Infect. Immun. 39:1072–1079.

    PubMed  CAS  Google Scholar 

  70. Zweerink, H. J., Gammon, M. D., Hutchinson, C. E, Jackson, J. J., Pier, G. B., Puckett, J. M., Sewell, T. J., and Sigal, N. H., 1988, χ-linked immunodeficient mice as a model for testing the protective efficacy of monoclonal antibodies against Pseudomonas aeruginosa,Infect. Immun. 56:1209–1214.

    PubMed  CAS  Google Scholar 

  71. Lopez, C., 1975, Genetics of natural resistance to herpes virus infections in mice, Nature 258: 152–153.

    Article  PubMed  CAS  Google Scholar 

  72. Kirkland, T. N., and Fierer, J., 1983, Inbred mouse strains differ in resistance to lethal Coccidioides immitis infection, Infect. Immun. 40:912–916.

    PubMed  CAS  Google Scholar 

  73. Wassom, D. L., David, C. S., and Gleich, G. J., 1979, Genes within the major histocompatibility complex influence susceptibility to Trichinella spiralis in the mouse, Immunogenetics 9:491–496.

    Article  Google Scholar 

  74. Lissner, C. R., Weinstein, D. L., and O’Brien, A. D., 1985, Mouse chromosome 1 Ity locus regulates microbicidal activity of isolated peritoneal macrophages against a diverse group of intracellular and extracellular bacteria, J. Immunol. 135:544–547.

    PubMed  CAS  Google Scholar 

  75. Groves, M. G., Rosenstreich, D. K., Taylor, B. A., and Osterman, J. V, 1980, Host defenses in experimental scrub typhus: Mapping the gene that controls natural resistance in mice,J. Immunol. 125:1395–1399.

    PubMed  CAS  Google Scholar 

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  1. Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA

    Richard S. Berk

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  1. University of Pisa, Pisa, Italy

    Mario Campa  & Mauro Bendinelli  & 

  2. University of South Florida, Tampa, Florida, USA

    Herman Friedman

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Berk, R.S. (1993). Genetic Regulation of the Murine Corneal and Non-Corneal Response to Pseudomonas aeruginosa . In: Campa, M., Bendinelli, M., Friedman, H. (eds) Pseudomonas aeruginosa as an Opportunistic Pathogen. Infectious Agents and Pathogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3036-7_10

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  • DOI: https://doi.org/10.1007/978-1-4615-3036-7_10

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