Abstract
An osmotic pulse was used to modify red blood cells (RBC) from two patients with sickle cell disease, resulting in an increased volume and decreased hemoglobin content. This treatment yielded cells which were divided into two populations, one in which RBC had markedly decreased hemoglobin concentration and another in which the cells appeared to be unmodified. Morphological sickling at low oxygen partial pressure was markedly decreased. However, there was no evidence for increased RBC lifespan when the 51Crlabeled, modified cells were reinfused.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
H.R. Sunshine, J. Hofrichter and W.A. Eaton, Requirements for therapeutic inhibition of sickle hemoglobin gelation, Nature. 275:238(1978).
G.M. Brittenham, A.N. Schechter and C.T. Noguchi, Hemoglobin S polymerization: Primary determinant of the hemolytic and clinical severity of the sickling syndromes, Blood. 65:183(1985).
T.B. Haddy and O. Castro, Overt iron deficiency in sickle cell disease, Arch Intern Med. 142: 1621(1982).
R.M. Rosa, B.E. Bierer, R. Thomas, J.S. Stoff, M. Kruskall, S. Robinson, H.F. Bunn and F.H. Epstein, A study of induced hyponatremia in the prevention and treatment of sickle cell crisis. N Engl J Med. 303:1138(1980).
G.P. Rodgers, G.J. Dover, C.T. Noguchi, A.N. Schechter and A.W. Nienhuis, Hematologic responses of patients with sickle cell disease to treatment with hydroxyurea, New Eng J Med. 322:1037(1990).
R.S. Franco, R. Barker, S. Novick, M. Weiner and O.J. Martelo, The effect of inositol hexaphosphate on the transient behavior of red cells following a DMSO-induced osmotic pulse, J Cell Phys. 129:221(1986).
R.S. Franco, R.L. Barker, G. Mayfield, E.B. Silberstein and M. Weiner, The in vivo survival of human red cells with low oxygen affinity prepared by the osmotic pulse method of inositol hexaphosphate incorporation, Transfusion. 30:196(1990).
R.S. Franco and R.L. Barker, Modification of the oxygen affinity and intracellular hemoglobin concentration of normal and sickle cells by means of an osmotic pulse, J Lab Clin Med. 113:58(1989).
R.S. Franco, K. Wagner, M. Weiner and O.J. Martelo, Preparation of low affinity red cells with dimethyl sulfoxide mediated inositol hexaphosphate incorporation: Hemoglobin and ATP recovery using a continuous flow method, Amer. J. Hematology 17:393(1984).
U. Galili, M.R. Clark and S.B. Shohet, Excessive binding of natural anti-alphaÂgalactosyl immunoglobulin G to sickle erythrocytes may contribute to extravascular cell destruction. J Clin Invest. 77:27(1986).
V. Russo, R. Barker-Gear, R. Gates and R. Franco, Studies with biotinylated RBC: (1) Use of flow cytometry to determine posttransfusion survival and (2) Isolation using streptavidin conjugated magnetic beads, This volume.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Franco, R. et al. (1992). Sickle Cells Modified by an Osmotic Pulse in the Presence of Inositol Hexaphosphate Have Decreased Intracellular Hemoglobin Concentration and Decreased in Vitro Sickling without Prolonged in Vivo Survival. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_39
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3030-5_39
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6321-7
Online ISBN: 978-1-4615-3030-5
eBook Packages: Springer Book Archive