Abstract
Erythrocytes labeled with fluorescent membrane markers were reinfused into mice. Blood samples removed periodically were analyzed by flow cytometry for survival of labeled cells. Normal erythrocytes labeled with the markers persisted in the circulation for 45 days. A fraction of lysed and resealed erythrocytes labeled with marker were rapidly cleared from the circulation; the remainder were removed at approximately the same rate as normal cells in the same animal labeled with marker of another color. Lysis in a minimal volume of buffer and addition of ATP during lysis both increased the fraction of cells surviving initial clearance.
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© 1992 Springer Science+Business Media New York
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Schlegel, R.A., Lumley-Sapanski, K., Williamson, P. (1992). Single Cell Analysis of Factors Increasing the Survival of Resealed Erythrocytes in the Circulation of Mice. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_16
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DOI: https://doi.org/10.1007/978-1-4615-3030-5_16
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6321-7
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