Summary
The osmotic fragility curves of isotonic rat RBCs, studied at pH 8 to avoid the Hb insolubility, are similar to those in humans at pH 7.4. Hypotonized rat RBCs, either directly or dialysed (0–24 h), are more hemolysis-resistant than isotonic rat RBCs. The discocytestomatocyte-spherocyte transformation can be observed with scanning electron microscopy. Protein crosslinking with dimethyl suberimidate can stabilize RBCs. The crosslinking level (60%), the cellular yield (80%), the mechanical and hemolytic resistance and the protective effect of enzyme activities, were studied in crosslinked or crosslinked-permeabilized RBCs after digitonin treatment. The normal discocytic shape of RBCs under scanning electron microscopy becomes stomatocytic in crosslinked and crosslinked-permeabilized RBCs with an erosioned surface.
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Luque, J., Garín, M.I., Sanz, S., Ropero, P., Pinilla, M. (1992). Properties of Hypotonized, Crosslinked and Crosslinked- Permeabilized Rat Erythrocytes as Potential Carrier Systems. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_10
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