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Endocytic Components: Identification and Characterization pp 95–123Cite as

Quantitative Fluorescence Techniques for the Characterization of Endocytosis in Intact Cells

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Part of the book series: Subcellular Biochemistry ((SCBI,volume 19))

Abstract

Fluorescence techniques have been used extensively in the biological sciences, and have been shown to be powerful tools for studying cell biology. Fluorescence has been used mainly for qualitative study in fixed preparations, as in immunofluorescence which has proved useful in the identification and localization of organelles and cellular proteins. Recently, a number of techniques have been developed for quantitative studies in both fixed and living cells.*

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Authors and Affiliations

  1. Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, New York, 10032, USA

    Nita H. Salzman & Frederick R. Maxfield

  2. Department of Pharmacology, New York University School of Medicine, New York, New York, 10016, USA

    Nita H. Salzman

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  1. Nita H. Salzman
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  2. Frederick R. Maxfield
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Editors and Affiliations

  1. Department of Anatomy, McGill University, Montreal, Canada

    J. J. M. Bergeron

  2. Institute of Zoology, University of Mainz, Mainz, Germany

    J. R. Harris

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Salzman, N.H., Maxfield, F.R. (1993). Quantitative Fluorescence Techniques for the Characterization of Endocytosis in Intact Cells. In: Bergeron, J.J.M., Harris, J.R. (eds) Endocytic Components: Identification and Characterization. Subcellular Biochemistry, vol 19. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3026-8_4

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