Summary
Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.
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© 1994 Springer Science+Business Media New York
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Opstelten, D.J.E., Horzinek, M.C., Rottier, P.J.M. (1994). Complex Formation between the Spike Protein and the Membrane Protein during Mouse Hepatitis Virus Assembly. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_30
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DOI: https://doi.org/10.1007/978-1-4615-2996-5_30
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