Abstract
The presence of subgenomic mRNAs (sgRNAs) in virions of infectious bronchitis virus was examined by probing Northern blots of RNA extracted from vinons using as a probe a cDNA of the 3’-terminal nucleocapsid protein (N) gene. The sgRNAs were readily detected even after extensive purification of virions and after RNase A treatment of virions. The molar ratio of gRNA to each sgRNA was in the range 25 to 400 for IBV-M41 and 10 to 30 for IBV-Beaudette. After comparison with the molar ratios of genomic to intracellular viral sgRNAs it was estimated that the efficiency of incorporation of gRNA into virions was approximately 100 to 500-fold greater than for sgRNAs in the case of M41 and 20 to 100-fold for Beaudette, depending on the sgRNA species. It is concluded that sgRNAs can be present within IBV virions. Approximately 1 in 3 Beaudette virions and 1 in 20 M41 particles might contain a single copy of one sgRNA.
Chapter PDF
References
P.B. Sethna, S-L Hung and Brian, D.A. Proc.Natl.Acad.Sci. USA. 86:5626 (1989).
M.A. Hofmann, P.B. Sethna and D.A. Brian. J.Virol. 64:4108 (1990).
P.B. Sethna, M.A. Hofmann and D.A. Brian. J.Virol. 65:320 (1991).
S. Makin, F. Taguchi and K. Fujiwara. Virology 133:9 (1984).
P. Chomczynski and N. Sacchi. Anal.Biochem. 162:156 (1987).
S. Makino, K. Yokomori and M.C. Lai. J. Virol. 64:6045 (1990).
R.G. van der Most, P.J. Bredenbeek and W.J.M. Spaan. J. Virol. 65:3129 (1991).
J.A. Fosmire, K. Hwang and S. Makino. J. Virol. 66:3522 (1992).
S.G. Sawicki and D.L. Sawicki. J. Virol. 64:1050 (1990).
J.G. Keck, G.K. Matsushima, S. Makino, J.O. Fleming, D.M. Vannier, S.A. Stohlman and M.M.C. Lai. J.Virol. 62:1810 (1988).
D. Cavanagh and P.J. Davis. J. Gen. Virol. 69:621 (1988).
J.G. Kusters, E.J. Jager, H.G.M. Niesters and B.A.M. van der Zeijst. Vaccine 8:605 (1990).
D. Cavanagh, P.J. Davis and J.K.A. Cook. Avian Pathol. 21:413 (1992).
S. Makin, M. Joo and J.K. Makino. J.Virol. 65:6031 (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Cavanagh, D., Shaw, K., Xiaoyan, Z. (1994). Analysis of Messenger RNA within Virions of IBV. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_20
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2996-5_20
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6305-7
Online ISBN: 978-1-4615-2996-5
eBook Packages: Springer Book Archive