Abstract
Thrombin and adenosine diphosphate (ADP) are perhaps the two most important activators of platelet function (Stormorken, 1986). Both agents elicit a rapid “shape change” where platelet discoid shape is lest and pseudopodia are extended, followed by aggregation. Thrombin also induces massive granule secretion under a variety of conditions, while ADP is much less potent (Mustard et al., 1975). There is therefore considerable functional similarity for both agents and much research has been directed to understanding the biochemical basis for how they cause the initial events in signal transduction, leading to “shape change”, activation of the glycoprotein IIb/Illa receptor with binding of fibrinogen, and resulting platelet aggregation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Agranoff, B.W., Murthy, P. and Seguin, E.B., 1983, Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol biphosphate in human platelets, J Biol Chem, 258:2076.
Authi, K.S., Hughes, K. and Crawford, N., 1989, High incorporation of [3Н] inositol into phosphoinositides of human platelets during reversible electropermeabilization, FEES Lett, 254:52.
Bell, R.L. and Majerus, P.W., 1980, Thrombin-induced hydrolysis of phosphatidylinositol in human platelets, J Biol Chem, 255:1790.
Breer, H., Boekhoff, I. and Tareilus, E., 1990, Rapid kinetics of second messenger formation in olfactory transduction, Nature, 345:65.
Daniel, J.J., Dangelmaier, C.A. and Smith, J.B., 1987, Formation and metabolism of inositol 1,4,5trisphosphate in human platelets, Biocheml, 246:109.
Daniel, J.L., Dangelmaier, C.A., Selak, M. and Smith, J.B., 1986, ADP stimulates IP3 formation in human platelets, FEES Lett, 206:299.
Davies, T.A., Weil, G.J. and Simons, E.R., 1990, Simultaneous flow cytometric measurements of thrombin-induced cytosolic pH and Ca2+ fluxes in human platelets, J Biol Chem, 265:11,522.
Downes, C.P. and MacPhee, C.H., 1991, Myo-inositol metabolites as cellular signals, Eur J Biochem, 193:1.
Feliste, R., Simon, M.F., Chap, H., Douste-Blazy, L., Defreyn, G. and Maffrand, J.P., 1988, Effect of PCR 4099 on ADP-induced calcium movements and phosphatidic acid production in rat platelets, Biochem Pharmacol,37:2559.
Finch, E.A., Turner, T.J., and Golden, S.M., 1991, Calcium as a coagonist of inositol 1,4,5-trisphosphateinduced calcium release, Science, 252:443.
Fisher, G.J., Bakshian, S. and Baldassare, J.J., 1985, Activation of human platelets to ADP causes a rapid rise in cytosolic free calcium without hydrolysis of phosphatidylinositol-4,5-bisphosphate, Biochem Biophys Res Commun, 129:958.
Gachet, C. and Cazenave, J.P., 1991, ADP-induced blood platelet activation: a review, Nouv Rev Fr Hematol, 33:347.
Gear, A.R.L., 1982, Rapid reactions of platelets studies by a quenched-flow approach: aggregation kinetics, J Lab Clin Med, 100:866.
Gear, A.R.L. and Burke, D., 1982, Thrombin-induced secretion of serotonin from platelets can occur in seconds, Blood, 60:1231.
Gear, A.R.L., 1984, Rapid platelet morphological changes visualized by scanning-electron microscopy: Kinetics derived from a quenched-flow approach, Br J Haematol, 56:387.
Hallam, T.J., Sanchez, A. and Rink, T.J., 1984, Stimulus-response coupling in human platelets. Changes evoked by platelet-activating factor in cytoplasmic free calcium monitored with the fluorescent calcium indicator quint, Biochem J, 218:819.
Hallam, T.J. and Rink, T.J., 1985, Responses to adenosine diphosphate in human platelets loaded with the fluorescent calcium indicator quin2, J Physiol. (Lond), 368:131.
Harootunian, A.T., Kao, J.P.Y., Parenjape, S. and Tsien, R.Y., 1991, Generation of calcium oscillations in fibroblasts by positive feedback between calcium and IP3, Science, 251:75.
Haver, V.M. and Gear, A.R.L., 1981, Functional fractionation of platelets, J Lab Clin Med, 97:187.
Jones, G.D. and Gear, A.R.L., 1988, Sub-second calcium dynamics in ADP and thrombin-stimulated platelets: A continuous-flow approach using Indo-1, Blood, 71:1539.
Jones, G.D. and Gear, A.R.L., 1990, Rapid blood platelet activation: Continuous and quenched-flow versus stopped-flow approaches, Biochem J, 265:305.
Lloyd, J.V., Nishizawa, E.E., Joist, J.H. and Mustard, J.F., 1973, Effect of ADP-induced aggregation on 32РО4 incorporation into phosphatidic acid and the phosphoinositides of rabbit platelets, Br] Haematol, 24:589.
Mauro, G., Chap, H. and Douste-Blazy, L., 1979, Characterization and properties of a phosphatidyl-inositol phosphodiesterase (phospholipase C) from platelet cytosol, Febs Lett,100:367.
Michell, R.H., 1975, Inositol phospholipids and cell surface receptor function, Biochem Biophys Acta, 415:81
Murphy, C.T., Elmore, M., Kellie, S. and Westwick, J., 1991, The relationship between cytosolic Cat+, sn1,2-diacylglycerol and inositol 1,4,5-trisphosphate elevation in platelet-activating-factorstimulated rabbit platelets, Biochem J, 278:255.
Mustard, J.F., Perry, D.W., Kinlough-Rathbone, R.L. and Packham, M.A., 1975, Factors responsible for ADP-induced release reaction of human platelets, Aml Physiol, 228:1757.
Rittenhouse-Simmons, S.E., 1979, Production of diglyceride from phosphatidylinositol in activated human platelets. Clin Invest, 63:680.
Sage, S.O. and Rink, T.J., 1986, Kinetic differences between thrombin-induced and ADP-induced calcium influx and release from internal stores in fuгa-2-loaded human platelets. Biochem Biophys Res Commun, 136:1124.
Sage, S.O. and Rink, T.J., 1987, The kinetics of changes in intracellular calcium concentration in fura-2loaded human platelets, J Bin! Chem, 262:13364.
Sage, S.O., Reast, R. and Rink, T.J., 1990, ADP evokes biphasic Cat+ influx in fuгa-2-loaded human platelets., Biochem J, 265:675.
Stormorken, H., 1986, Platelets in hemostasis and thrombosis, in: “Platelet responses and metabolism”, Vol. 1, pp. 4–29, “Responses”, H. Holmsen, ed. CRC Press, Inc., Boca Raton, Florida.
Streb, H., Irvine, R.F., Berridge, M.J. and Schulz, I., 1983, Release of Са2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate, Nature,306:67.
Sweatt, J.D., Blair, I.A., Cragoe, E.J. and Limbird, L.E., 1986, Inhibitors of Na+/H+ exchange block epinephrine and ADP-induced stimulation of human platelet phospholipase C by blockade of arachidonic acid release at a prior step, J Biol Chem, 261:8660.
Vickers, J.D., Kinlough-Rathbone, R.L. and Mustard, J.F., 1982, Changes in phosphatidyl-4,5bisphosphate 10 seconds after stimulation of washed rabbit platelets with ADP, Blood, 60:1247.
Vickers, J.D., Kinlough-Rathbone, R.L. and Mustard, J.F., 1986, The decrease in phosphatidylinositol-4,5bisphosphate in ADP-stimulated washed rabbit platelets is not primarily due to phospholipase C activation, Biochem J, 237:327.
Vickers, J.D., Kinlough-Rathbone, R.L., Packham, M.A. and Mustard, J. F., 1990, Inositol phospholipids metabolism in human platelets stimulated bu ADP, Eur J Biochem, 193:521.
Wakui, M., Potter, B.V.L. and Petersen, O.H., 1989, Pulsatile intracellular calcium release does not depend on flutuations in inositol trisphosphate concentration, Nature, 339:317.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Gear, A.R.L., Raha, S. (1993). Calcium Signalling and Phosphoinositide Metabolism in Platelets: Subsecond Events Revealed by Quenched-Flow Techniques. In: Authi, K.S., Watson, S.P., Kakkar, V.V. (eds) Mechanisms of Platelet Activation and Control. Advances in Experimental Medicine and Biology, vol 344. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2994-1_5
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2994-1_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6304-0
Online ISBN: 978-1-4615-2994-1
eBook Packages: Springer Book Archive