Abstract
As cocaine may affect progression of the Human Immunodeficiency Virus (HIV) infection to Acquired Immune Deficiency Syndrome (AIDS), we used a murine model of AIDS (MAIDS) induced by LP-BM5 murine leukemia virus to examine cocaine’s possible role as a cofactor for secondary parasitic infections. Dissimilarities between the sexes were observed both in the absence and presence of the cocaine. The retrovirus-infected female mice had a much higher rate of Cryptosporidiosis than the retrovirus-infected male mice. Female, but not male, retrovirus-infected mice showed approximately 20-fold more Cryptosporidium per villus section than controls. Compared to respective gender controls, male and female animals infected with the retrovirus infection manifested a heightened Cryptosporidium oocysts count regardless of cocaine treatment. Overall, female groups incurred a higher incidence of infection compared to respective male groups. To determine the role of cocaine, groups of male and female C57BL-6 mice of similar age were treated with cocaine for 4 weeks followed by termination. Cocaine synergized with retrovirus infection in female mice to cause a 30-fold increase in the number of oocyst present. The spleen size and weight of female mice was significantly greater than uninfected controls or male mice. However, due to the very slow progression to murine AIDS in the males, parasite resistance was retained, including in cocaine treated C57BL-6 mice. Thymus cell number in the retrovirus-infected female mice decreased significantly in comparison to uninfected female controls. Continued resistance to the parasite in male mice and its loss in female mice was due to the rate of immunosuppression and thus development of retrovirus-induced murine AIDS.
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References
E. E. Tyzzer, A sporozoan found in the peptic glands of the common mous, Proc. Soc. Exp. Biol. Med. 5:12 (1907).
M. H. Hart, R. Kruger, S. Nielson, and S.S. Kaufman. Acute self-limited colitis associated with Cryptosporidium in an immunocompetent patient, J. Pediatr. Gastroenterol. Nutr. 8:401(1989).
R. Fayer, and B.L.P. Unger, Cryptosporidium SPP and Cryptosporidiosis, Microbiol Rev. 50:458 (1986).
Chieffi, Cryptosporidiosis among patients with acquired immunodeficiency syndrome (AIDS) in theR. M.D. Dias, A.C.S. Mangini, D.M.G.V. Torres, M.O.A. Corra, N. Lupetti, F.M.A. Corra, and P.P.country of Sao Paulo, Brazil, Rev. Inst. med. Trop. Sao Paulo 30:310 (1988).
B. L.P. Nuger, J.A. Burris, C.A. Quinn, and F. Finkelman, New mouse models for chronic Cryptosporidium infection in immunodeficient hosts, Infect. Immun. 58:961 (1990).
M. Armstrong, Cryptosporidiosis, Med. Lab. Sci. 44:280 (1987).
W. L. Current, N.R. Reese, J.V. Ernst, W.S. Bailey, M.B. Heyman, and W.M. Weinstein, Human cryptosporidiosis in immunocompetent and immunodefícient persons, Studies of an outbreak and experimental transmission, N. Eng. J. Med. 308:1252 (1983).
R. Soave, and D. Armstrong, Cryptosporidium and cryptosporidiosis, Rev. Infect. Dis. 8:1012 (1986).
C. Chrisp, W.C. Reid, H.G. Rush, MA. Sucknow, A. Bush, and M.J. Thomann, Cryptosporidiosis in Guinea pigs: An animal model, Infect. Immun. 586471990
S. K. Chattopadhyay, M. Makino, J.W. Harlety, and H.C. Morse III, Pathogenesis of MAIDS: a retrovirus-induced immunodeficiency disease of mice, in: “Immune-deficient animal in experimental medicine, Sixth International Workshop on Immune-Deficient Animals, B-Q. Wu, and J. Zeng, eds., Basel Karger, p. 12 (1989.(1988).
R. R. Watson, Murine models for acquired immune deficiency syndrome, Life Sci. 44:1–xiii (1989).
L. A. Salzman, ed. “Animal Models of Retrovirus Infection and their Relationship to AIDS”, Academic Press, Orlando, FL, (1986).
D. E. Mosier, R.A. Yetter, and H.C. Morse III, Retroviral induction of acute lymphoproliferative disease and profound immunosuppression in adult C47BL/6 mice, J. Exp. Med. 161:766 (1985).
S. P. Ninken, T.N. Fredrickson, J.W. Hartley, RA. Yetter, and H.C. Morse III., Evaluation of B-celllineage lymphomas in mice with retrovirus-induced immunodeficiency syndromes, MAIDS, J.Immunol. 140:1123 (1988).
R. M.L. Buller, R A. Yetter, T.N. Fredrickson, and H.C. Morse III, Abrogation of resistance to severemouse pox in C57BL/6 mice infected with LP-BM5 murine leukemia viruses, J. Virol. 61:383 (1987).
D. E. Mosier, Animal models for retrovirus-induced immunodeficiency disease, Immunol. Invest. 15:233 (1986)
D. E. Mosier, RA. Yetter, and H.C. Morse III, Functional T-lymphocytes are required for a murineretrovirus-induced immunodeficiency disease (MAIDS), J. Exp. Med. 165:1737 (1987).
A. Cerny, A.W. Hugin, R.R. Hardy, K. Hayakawa, R.M. Zinkernagel, M. Makino, and H.C. Morse III, B cells are required for induction of T-cell abnormalities in murine retrovirus-induced immunodeficiency model, J. Exp. Med. 171:315 (1990).
H. Darban, J. Enriquez, C.R. Sterling, M.C. Lopez, G. Chen, R.R. Watson, and M. Abaszadegan, Cryptosporidiosis facilitated by murine retroviral infection with LP-BM5, J. Infect. Dis. 164:741(1991)
R. Taghi-Kilani, L. Sekla, and K.T. Hayglass, The role of humoral immunity in Cryptosporidium SPP infection studies with B cell-depleted mice, J. Immunol. 145:1571 (1990).
B. Watzl, and R.R. Watson, Immunomodulation by cocaine - A neuroendocrine mediated response, Life Sci 46:1319 (1990).
M. Holsapple, and A. Munson, Immunotoxicology of abused drugs, in: “Immunotoxicol. And Immunopharmacol.” J. Dean, ed.,Raven Press, NY, pp. 381–392 (1985).
D. W. Ou, M. Shen, and Y-D Luo, Effect of cocaine on the immune system of BALB/c mice, Clin. Immunol. Immunopathol. 52:305 (1989).
G. Chen, and R.R. Watson, Modulation of tumor necrosis factor and gamma interferon production by cocaine and morphine in aging mice infected with LP-BMS, a murine retrovirus, J. Leuk. Biol. 50:349 (1991)
S. Chattopadhyay, K. Sisio, M. Makino, J.W. Hartley, and H.C. Morse III, Pathogenesis of MAIDS, a retrovirus-induced immunodeficiency disease of mice, Immunodeficient Animals in Experimental Med.12:18 (1989).
12:18 (1989). 26. M. J. Arrowood, J.R. Mead, J.L. Mahrt, and C.R. Sterling, Effects of immune colostrum and orally administered anti-sporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice, Infect. Immun. 57 2283 1989
J. A. Harp, and H.W. Moon, Susceptibility of mast cell-deficient w/wV mice to Cryptosporidium parvum, Infect. Immun. 59:718 (1991).
J. A. Ernest, B.L. Balgburn, D.L. Lindsey, and W.L. Current, Infection dynamics of Cryptosporidium parvum in neonatal mice, J. Parasitol. 72:796–98 (1986).
J. A. Harp, M.W. Wannemuehler, D.B. Woodmansee, andH.W. Moon, Susceptibility of germ free or antibiotic-treated adult mice to Cryptosporidium parvum, Infect. Immun. 56:2006 (1988).
D. P. Kotler, H.P. Gaetz, M. Lange, and P.R. Holf, Enteropathy associated with the acquired immunodeficiency syndrome, Ann. Intern. Med. 101:421(1984).
T. J. Quinn, Protozoan infections, in: “Gastrointestinal infections in AIDS” Smith PD, moderator, Ann. Intern. Med. 116:63 (1992).p 66–68.
R. M. Pillai, and R.R. Watson, AIDS: Disease progression and immunomodulation by drugs of abuse and alcohol, AIDS Med Report 4:25 (1991).
R. M. Pillai, and R.R. Watson, In vitro immunotoxico}ogy and immunopharmacology: Studies on drugs of abuse, Toxicol. Letters 53:269 (1990).
R. R. Watson, Cofactors in HIV infection: Progression to AIDS and increased susceptibility to disease, AIDS Med Report 4:74 (1991).
T. V. Ellerbrock, T.J. Bush, M.E. Chamberland, and M.J. Oxtoby, Epidemiology of women with AIDS in the United States, 1981, through 1990. A comparison with heterosexual men with AIDS, JAMA265:2971 (1991).
T. Beardsley, Cross reaction, Could AIDS really be an autoimmune disease? Sci. Am. 265:56 (1991).
G. W. Hoffman, T.A. Kion, and M.D. Grant, An idiotypic network model of AIDS immunopathogene-sis, Proc. of the Nat. Acad. Sci. 88 3060 1991
W. J. Morrow, D.A. Isenberg, R.E. Sobol, R.B. Strieker, and T. Kieber-Emmons, AIDS virus infection and autoimmunity: a perspective of the clinical, immunological, and molecular origins of the autoallergic pathologies associat ed with HIV disease, Clin Immun. and Immunopath. 58 163 1991
A. M. Solinger, and E.V. Hess, Induction of autoantibodies by human immunodeficiency virus infection and their significance, Rheum. Dis. Clin. of NAm. 17:157 (1991).
A. W. Hugin, M.S. Vaccio, and H.C. Morse III, A virus encoded “superantigen” in a retrovirus-induced immunodeficiency syndrome of mice, Sci. 252:424 (1991).
J. Laurence, H. Cooke, and S.K. Sikder, Effects of tamoxifen on regulation of viral replication and human immunodeficiency virus (HIV) long terminal repeat-directed transcription cells chronically infected with HIV-1, Blood 75:696 (1990).
L. Imberti, A. Sottini, A. Bettinardi, M. Puoti, and D. Primi, Selective depletion in HIV infection of T cells that bear specific T cell receptor V beta sequences, Sci. 254:860 (1991).
C. G. Drake, and B.L. Kotzin, Superantigens: Biology, immunology, and potential role in disease, J. Clin. Immunol. 12:149 (1992).
F. Homo-Delarche, F. Fitzpatrick, N. Christeff, E.A. Nunez, J.F. Bach, and M. Dardenne, Sex steroids, glucocorticoids, stress and autoimmunity, J. Ster. Biochem. Mol. Biol. 9:619 (1991).
C. J. Grossman, G.A. Roselle, and C.L. Mendenhall, Sex steroid regulation of autoimmunity, J. Ster. Biochem. Mol. Biol. 11:649 (1991).
A. H. Schuurs, and H.A. Verheul, Sex hormones and autoimmune disease, Brit. J. Rheum. 28:59 (1989).
N. Talal, Autoimmunity and sex revisited, Clin. Immunol. Immunopath. 53:355 (1989).
N. Talal, and S.A. Ahmed, Sex hormones, CD5 + (Lyl+) B- cells, and autoimmune diseases, Israel J. Med. Sci. 24:725 (1988).
S. A. Ahmed, and N. Talal, Sex hormones and the immune system--Part 2, Animal data, Baillieres Clin. Rheumat. 4:13 (1990).
A. H. Schuurs, and HA. Verheul, Effects of gender and sex steroids on the immune response, J. of Ster. Biochem. 35:157 (1990).
H. Carlsten, R. Holmdahl, A. Tarkowski, and LA. Nilsson, Oestradiol-and testosterone-mediated effects on the immune system in normal and autoimmune mice are genetically linked and inherited asdominant traits, Immunol. 68:209 (1989).
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Darban, H., Watson, R.R., Alak, J., Thomas, N. (1993). Cocaine Facilitation of Cryptosporidiosis by Murine AIDS in Male and Female C57/BL/6 Mice. In: Friedman, H., Klein, T.W., Specter, S. (eds) Drugs of Abuse, Immunity, and AIDS. Advances in Experimental Medicine and Biology, vol 335. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2980-4_20
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