Abstract
A computer program, MetaCon, has been developed which fully automates all stages of carrying out a control analysis to give values for the flux control, concentration control and branch-point distribution control coefficients of a metabolic pathway. The program input is a text file containing processing instructions and the pathway details. The pathway is analyzed for the presence of branches and moiety-conserved cycles. The results of this stage are used along with inferred and additional elasticity terms to build an elasticity matrix of the form of the Fell and Sauro matrix method1,2, though the process involves symbolic evaluation of Reder’s equations3. Inversion of the matrix yields the expressions for the control coefficients in terms of the elasticities, metabolite concentrations and fluxes which appear in the elasticity matrix. All calculations are carried out algebraically rather than numerically, so that if the values of one or more variables are unknown, the control coefficients will be produced as polynomials in terms of the unknown(s).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
D.A. Fell and H.M. Sauro, Metabolic control and its analysis: additional relationships between elasticities and control coefficients, Eur. J. Biochem. 148:555–561 (1985).
H.M. Sauro, J.R. Small and D.A. Fell, Metabolic control and its analysis: extensions to the theory and matrix method, Eur. J. Biochem. 165:215–221 (1987).
C. Reder, Metabolic control theory: a structural approach, J. theor. Biol. 135:175–201 (1988).
J.R. Small and D.A. Fell, Sensitivity of control coefficients to elasticities, Eur. J. Biochem. 191:413–420 (1990).
B.W. Kernighan and D.M. Ritchie. “The C Programming Language,” Prentice-Hall, Englewood Cliffs (N.J.) (1988).
A.K. Groen. “Quantification of Control in Studies on Intermediary Metabolism,” Ph.D. Thesis, University of Amsterdam (1984).
A.K. Groen, C.W.T. van Roermund, R.C. Vervoom and J.M. Tager, Control of gluconeogenesis in rat liver cells. Flux control coefficients of the enzymes in the gluconeogenic pathway in the absence and presence of glucagon, Biochem. J. 237:379–389 (1986).
A.K. Groen and H.V. Westerhoff, Modern control theories: a consumers’ test, in: “Control of Metabolic Processes,” A. Cornish-Bowden and M. L. Cárdenas, eds, Plenum Press, New York (1990).
A.K. Groen, R.C. Vervoorn, R. Van der Meer and J.M. Tager, Control of gluconeogenesis in rat liver cells. 1. Kinetics of the individual enzymes and the effect of glucagon, J. Biol. Chem. 258:14346–14353 (1983).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Thomas, S., Fell, D.A. (1993). MetaCon - A Computer Program for the Algebraic Evaluation of Control Coefficients of Metabolic Networks. In: Schuster, S., Rigoulet, M., Ouhabi, R., Mazat, JP. (eds) Modern Trends in Biothermokinetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2962-0_75
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2962-0_75
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6288-3
Online ISBN: 978-1-4615-2962-0
eBook Packages: Springer Book Archive