Abstract
The mitochondria of Euglena have an outstanding capacity to oxidize cytosolic NADH as efficiently coupled to oxidative phosphorylation as matrix NADH: both paths reveal the same sensitivity to rotenone and identical P/O values1. However, they strongly differ in the rotenone sensitivity of ferricyanide reduction, indicating two separate NADH-oxidases at the inside and the outside of the inner mitochondrial membrane (see Fig. 1).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
U. W. Kümmel and K. Brinkmann, The oxidation of exogenous NADH by mitochondria of Euglena gra cilis, Planta 176:261–268 (1988).
Y. Hayashi and K. Ueda, The shape of mitochondria and the number of mitochondrial nucleotides during the cell cycle of Euglena gracilis, J. Cell Sci. 43:137–166 (1989).
R. Heinrich and T. A. Rapoport, A linear steady-state treatment of enzymatic chains; general properties, control and effector strength, Eur. J. Biochem. 42:89–95 (1974).
A. K. Groen, R. J. A. Wanders, H. V. Westerhoff, R. van der Meer and J. M. Tager. Quantification of the con-tribution of various steps to the control of mitochondrial respiration, J. Biol. Chem. 257:2754–2757 (1982).
M. D. Brand, R. P. Hafner and G. C. Brown, Control of the respiration in non-phosphorylating mitochondria is shared between the proton leak and the respiratory chain, Biochem. J. 255:535–539 (1988).
G. C. Brown, R. P. Hafner and M. D. Brand, A ‘top-down’ approach to the determination of control coefficients in metabolic control theory, Eur. J. Biochem. 188:321–325 (1990).
A. C. Padovan, I. B. Dry and J. T. Wiskich, An analysis of the control of phosphorylation coupled respiration in isolated plant mitochondria, Plant Physiol. 90:928–933 (1989).
A. Kesseler, P. Diolez, K. Brinkmann and M. D. Brand, Characterization of the control of respiration in potato tuber mitochondria using the top-down approach of metabolic control analysis, Eur. J. Biochem. 210:775–784 (1992).
R. P. Hafner, G. C. Brown and M. D. Brand, Analysis of the control of respiration rate, phosphorylation rate, proton leak rate and protonmotive force in isolated mitochondria using the top-down approach of metabolic control theory, Eur. J. Biochem. 188:313–319 (1990).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kesseler, A., Kisteneich, S., Brinkmann, K. (1993). Control of Respiration of Mitochondria from Euglena gracilis Determined by Means of Metabolic Control Analysis: Comparison of Oxidation of Matrix-and Cytosolic NADH. In: Schuster, S., Rigoulet, M., Ouhabi, R., Mazat, JP. (eds) Modern Trends in Biothermokinetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2962-0_50
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2962-0_50
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6288-3
Online ISBN: 978-1-4615-2962-0
eBook Packages: Springer Book Archive