Abstract
From many studies conducted to determine the stoichiometry of mitochondrial oxidative phosphorylation employing a remarkable variety of techniques and discarding several common systematic errors, there has been an increasing consensus in recent years. In mammalian mitochondria, the measured values of ATP/2e- were close to 1, 0.5 and 1 at the three “coupling sites”, respectively1,2. However, such determinations have been made under conditions where the electron flux through each respiratory unit is maximum (State 3) even if the number of functional units is changed by using inhibitor titration.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C.D. Stoner, Determination of the P/2e-stoichiometries at the individual coupling sites in mitochondrial oxidative phosphorylation, J. Biol. Chem. 262:10445–10453 (1987).
P.C. Hinkle, M.A. Kuman, A. Resetar and D.L. Harris, Mechanistic stoichiometry of mitochondrial oxidative phosphorylation, Biochemistry 30:3576–3582 (1991).
R. Ouhabi, M. Rigoulet and B. Guérin, Flux-yield dependence of oxidative phosphorylation at constant ΔµH+, FEBS Lett. 254:199–202 (1989).
B. Guérin, P. Labbe and M. Somlo, Preparation of yeast mitochondria (Saccharomyces cerevisiae) with good P/O and respiratory control ratios, Methods Enzymol. 55:149–159 (1979).
R. Ouhabi, M. Rigoulet, J.L. Lavie and B. Guérin, Respiration in non-phosphorylating yeast mitochondria. Roles of non-ohmic proton conductance and intrinsic uncoupling, Biochim. Biophys. Acta 1060: 293–298 (1991).
M. Rigoulet, J. Velours and B. Guérin, Substrate-level phosphorylation in isolated yeast mitochondria Eur. J. Biochem. 153:601–607 (1985).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Fitton, V., Rigoulet, M., Guérin, B. (1993). Mechanistic Stoichiometry of Yeast Mitochondrial Oxidative Phosphorylation: A Behavior of Working Engine. In: Schuster, S., Rigoulet, M., Ouhabi, R., Mazat, JP. (eds) Modern Trends in Biothermokinetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2962-0_46
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2962-0_46
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6288-3
Online ISBN: 978-1-4615-2962-0
eBook Packages: Springer Book Archive