Abstract
It frequently happens that three flow cytometry histograms with fluorescence intensities X, Y, and Z are related by shifting and stretching under the following circumstances:
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a)
X is observed at one subsaturating concentration of lectin or antibody and Y is observed at another concentration with the same cell type;
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b)
X is observed at saturating concentration of ligand with one cell preparation and Y is observed at saturating concentration of the same ligand, but with another cell preparation;
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c)
X is observed at saturating concentration of ligand with one cell line, Y is observed at saturating concentration of ligand with the same cell line but with another ligand and Z is observed when both ligands are added together.
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References
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© 1993 Springer Science+Business Media New York
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Bardsley, W.G., Kyprianou, E.K. (1993). The Interpretation of Flow Cytometry Profiles in Terms of Gene Dosage and Gene Expression. In: Schuster, S., Rigoulet, M., Ouhabi, R., Mazat, JP. (eds) Modern Trends in Biothermokinetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2962-0_24
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DOI: https://doi.org/10.1007/978-1-4615-2962-0_24
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