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Northern Blot Analysis of Sepiapterin Reductase mRNA in Mammalian Cell Lines and Tissues

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Chemistry and Biology of Pteridines and Folates

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 338))

Abstract

Recent evidence has shown that H4biopterin is synthesized in cells which undergo cytokine-directed proliferation and do not use H4biopterin as a hydroxylation cofactor. H4biopterin, in turn, enhances the proliferation of erythroleukemic cells and modulates various aspects of the interleukin-2-induced clonal expansion of T cells.1 In these cells the activity of sepiapterin reductase (SR) is in the range of 100 pmol min-1 mg-1.Lectin Stimulation of resting T cells causes a slowly progressing increase, starting from levels below detection and a transient enhancement of SR activity is found after treatment of activated T cells by interferon-γ plus interleukin-2.1 The NK-like human cell line YT and the murine erythroleukemic cell line B8/3 lack any SR activity. The molecular basis of SR regulation has not been explained in any of these cases.

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References

  1. I. Ziegler, K.Schott, M. Lübbert, F. Herrmann, U. Schwuléra, and A. Bacher, J. BiolChem.265:17026 (1991).

    Google Scholar 

  2. B.A. Citron, S.S. Milstien, I.C. Gutierres, R.A.Levine, B.L. Yanak, and S. Kaufman, Proc. Natl Acad.Sci. USA 87:6436(1990).

    Article  PubMed  CAS  Google Scholar 

  3. K. Schott, K. Brand, K. Hatakeyama, J. Maier, T. Werner, and I. Ziegler,Exp. Cell Res. 200:105 (1992).

    Article  PubMed  CAS  Google Scholar 

  4. J. Maier, K. Schott, T. Werner, A. Bacher, and I. Ziegler, Exp. CellRes. 204, in press (1993).

    Google Scholar 

  5. M. Gütlich, K. Schott, T. Werner, A. Bacher, and I. Ziegler, Biochim.Biophys. Acta 1171:133 (1992).

    Article  PubMed  Google Scholar 

  6. H. Ichinose, S. Katoh, T. Sueoka, K. Titani, K. Fujita, and T. Nagatsu, Biochem.Biophys. Res. Commun.179:183(1991).

    Article  PubMed  CAS  Google Scholar 

  7. R.A. Levine, J. Solus, S. Goustin, S. Tait, S.Demetriou, B. Citron, and S. Kaufman, in: Pterins and Neurogenic Amines in Neurology, Pediatrics andImmunology, N. Blau, H.-Ch. Curtius, R.A. Levine, and CottonR.G.H., eds., Lakeshore, Grosse Pointe, (1991).

    Google Scholar 

  8. K. Schott, M. Gütlich, and I. Ziegler, J. Cellul.Physiol., in press (1993).

    Google Scholar 

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Author information

Author notes

  1. Josef Maier

    Present address: Institut für Chemische Pflanzenphysiologie, Universität Tübingen, Corrensstr. 41, D-7400, Tübingen, Germany

Authors and Affiliations

  1. Institut für Klinische Molekularbiologie und Tumorgenetik, GSF-Gesellschaft für Umwelt-und Gesundheitsforschung, Marchioninistr. 25, D-8000, München, Germany

    Josef Maier, Karin Schott & Irmgard Ziegler

  2. Institut für Säugetiergenetik, GSF-Gesellschaft für Umwelt-und Gesundheitsforschung, Ingolstädter Landstr. 1, D-8042, Neuherberg, Germany

    Thomas Werner

  3. Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-8046, Garching, Germany

    Adelbert Bacher

Authors
  1. Josef Maier
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  2. Karin Schott
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  3. Thomas Werner
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  4. Adelbert Bacher
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  5. Irmgard Ziegler
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Editor information

Editors and Affiliations

  1. Dept of Pharmacology College of Medicine, Universily of South Alabama, Mobile, AL, 36688, USA

    June E. Ayling

  2. Dept. of Biochemistry College of Medicine, University of South Alabama, Mobile, AL, 36688, USA

    M. Gopal Nair

  3. College of Medicine, Universily of South Alabama, Mobile, AL, 36688, USA

    Charles M. Baugh

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© 1993 Springer Science+Business Media New York

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Maier, J., Schott, K., Werner, T., Bacher, A., Ziegler, I. (1993). Northern Blot Analysis of Sepiapterin Reductase mRNA in Mammalian Cell Lines and Tissues. In: Ayling, J.E., Nair, M.G., Baugh, C.M. (eds) Chemistry and Biology of Pteridines and Folates. Advances in Experimental Medicine and Biology, vol 338. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2960-6_39

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  • DOI: https://doi.org/10.1007/978-1-4615-2960-6_39

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6287-6

  • Online ISBN: 978-1-4615-2960-6

  • eBook Packages: Springer Book Archive

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