Advertisement

A Dendritic Cell Specific Determinant Present In Endosomes Is Involved in the Presentation of Protein Antigens

  • Toshiyuki Maruyama
  • Elisabeth C. M. Hoefsmit
  • Georg Kraal
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

Antigen presenting cells (APC) such as dendritic cells (DC), macrophages (M), and B lymphocytes present exogenous antigens on their cell surface to T cells, not as native proteins but as peptides, complexed with MHC-class II molecules (1). In contrast to macrophages where antigen processing occurs in endosomes (2). It is still unclear how and by which organelles the processing takes place in DC or B-cells (3). Using fluorescein conjugated protein antigen it has been shown that isolated splenic DC can take up some amount of protein antigen in intra-cytoplasmic organelles after over-night culturing. Furthermore, these DC were now able to prime T cells in situ (4). Therefore DC must have a small but relevant intra-cytoplasmic system for antigen processing.

Keywords

Dendritic Cell Antigen Present Cell Protein Antigen Antigen Processing Invariant Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    T. J. Braciale and V. L. Braciale, Antigen presentation: Structural themes and functional variations, Immunol. Today 12: 124 (1991).PubMedCrossRefGoogle Scholar
  2. 2).
    E. R. Unanue, Antigen-presenting function of the macrophage, Annu. Rev. Immunol. 2: 395 (1984).PubMedCrossRefGoogle Scholar
  3. 3).
    P. D. King and R. Katz, Mechanisms of dendritic cell function, Immunol. Today 11: 206 (1990).PubMedCrossRefGoogle Scholar
  4. 4).
    K. Inaba, P. Metlay, M. T. Crowley and R. M. Steinman, Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific MHOrestricted T cell in situ. J. Exp. Med. 172: 631 (1990).PubMedCrossRefGoogle Scholar
  5. 5).
    M. Breel, R. E. Mebius and G. Kraal, Dendritic cells of the mouse recognized by two monoclonal antibodies, Eur. J. Immunol. 17: 1555 (1987)PubMedCrossRefGoogle Scholar
  6. 6).
    E. Puré, K. Inaba, M. T. Crowley, L. Tardelli, D. W. Witmer-Pack, G. Fathman and R. M. Steinman, Antigen processing by epidermal Langerhans cells correlates with the level of biosynsthesis of major histocompatibility complex class II molecules and expression of invariant chain, J. Exp. Med. 172: 1459 (1990).PubMedCrossRefGoogle Scholar
  7. 7).
    W. L. Elliott, C. J. Steille, L. J. Thomas and R. E. Humphreys, An hypothesis on the binding of an amphipathic, helical sequence in Ii to the desetope of class II antigens, J. Immunol. 138: 2949 (1987).PubMedGoogle Scholar
  8. 8).
    H. K. Zieglar and E. R. Unanue, Decrease in macrophage antigen catabolism caused by ammonia and chloroquin is associated with inhibition of antigen presentation to T cells, Proc. Natl. Acad. Sci. USA 79: 175 (1982).CrossRefGoogle Scholar
  9. 9).
    G. Kraal, M. Breel, E. M. Janse and G. Bruin, Langerhans’ cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody, J. Exp. Med. 163: 981 (1986).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Toshiyuki Maruyama
    • 1
  • Elisabeth C. M. Hoefsmit
    • 1
  • Georg Kraal
    • 1
  1. 1.Department of Cell Biology Faculty of MedicineVrije UniversiteitBT AmsterdamThe Netherlands

Personalised recommendations