Immunophenotypic and Ultrastructural Differentiation and Maturation of Nonlymphoid Dendritic Cells in Osteopetrotic (op) Mice with the Total Absence of Macrophage Colony Stimulating Factor Activity
To examine the effect of macrophage colony stimulating factor (M-CSF) or CSF-1 on the differentiation and maturation of nonlymphoid dendritic cells (DCs) in vivo, the osteopetrotic mouse (op/op mouse) is a useful tool. This is because the op mutation is shown to be a defect in the coding region of the macrophage colony stimulating factor (Csfm) gene and because CSF-1 produced is nonfunctional, although this mouse does produce Csfm messenger RNA at normal levels.1 This mutation is transmitted by an autosomal recessive trait and homozygous (op/op) mice are characterized by the absence of incisors, a distinctly domed skull, a short tail, and a small body size.2 These phenotypic abnormalities become evident by ten days after birth. In addition to a marked reduction of osteoclasts, deficiencies of monocytes and monocyte-derived macrophages occur in op/op mice.3,4 All these result from the lack of CSF-1 activity. In a recent study, we found immature macrophages in various organs and tissues of op/op mice, suggesting that these CSF-1-independent macrophages are derived from granulocyte/macrophage colony forming cells (GM-CFCs) or earlier hematopoietic progenitors.5 However, little is known about the DCs of op/op mice, including interdigitating cells (IDCs) in the thymus or peripheral lymphoid tissues, epidermal Langerhans cells (LCs), or indeterminate dendritic cells (IDDCs).
KeywordsAutosomal Recessive Trait Birbeck Granule Peripheral Lymphoid Tissue Epidermal Sheet Thymic Medulla
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