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Recombinant GM-CSF Induces in Vitro Differentiation of Dendritic Cells from Mouse Bone Marrow

  • Christoph Scheicher
  • Maria Mehlig
  • Reinhard Zecher
  • Konrad Reske
  • Friedrich Seiler
  • Petra Hintz-Obertreis
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

The unprecedented functional capacity of dendritic cells (DC) in sensitizing resting T cells and their role in triggering T dependent immune responses attract increasing interest in this unique accessory cell population. Like macrophages (Mph) DC have been described to originate in the bone marrow (BM) (1). While the cytokine-promoted in vitro differentiation of Mph from BM-cells is well established, a convincing in vitro culture system for propagating mouse DC from BM-cells has not yet been reported. This work demonstrates the differentiation of DC from mouse bone marrow cells by a short term in vitro culture system supplemented with rGM-CSF.

Keywords

Dendritic Cell Mouse Bone Marrow Bone Marrow Culture Mouse Bone Marrow Cell Antigen Presentation Function 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Christoph Scheicher
    • 1
  • Maria Mehlig
    • 1
  • Reinhard Zecher
    • 1
  • Konrad Reske
    • 1
  • Friedrich Seiler
    • 2
  • Petra Hintz-Obertreis
    • 2
  1. 1.Institut für ImmunologieJohannes Gutenberg-UniversitätMainzGermany
  2. 2.Behringwerke Research LaboratoriesMarburgGermany

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