Abstract
It has been demonstrated recently that colonies of cells with dendritic morphology can be generated in-vitro in semi-solid cultures of both human bone marrow and peripheral blood mononuclear cells1. The cells express antigens typically found on skin Langerhans cells (CD1a, HLA-DR/DQ, CD4) and are functionally active in primary mixed leucocyte reactions (MLR). The colony forming cells are the earliest progenitors of dendritic/Langerhans cells so far identified and have been termed dendritic Langerhans colony forming units - DL-CFU. This bone marrow culture technique has now been applied in order to characterise further these progenitors of dendritic cells and to determine their regulation by growth factors in-vitro.
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References
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© 1993 Plenum Press, New York
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Reid, C.D.L., Tikerpae, J., Stackpoole, A. (1993). TNF and GM-CSF Dependent Growth of an Early Progenitor of Dendritic Langerhans Cells in Human Bone Marrow. In: Kamperdijk, E.W.A., Nieuwenhuis, P., Hoefsmit, E.C.M. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 329. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-2930-9_43
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DOI: https://doi.org/10.1007/978-1-4615-2930-9_43
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