TNF and GM-CSF Dependent Growth of an Early Progenitor of Dendritic Langerhans Cells in Human Bone Marrow
It has been demonstrated recently that colonies of cells with dendritic morphology can be generated in-vitro in semi-solid cultures of both human bone marrow and peripheral blood mononuclear cells1. The cells express antigens typically found on skin Langerhans cells (CD1a, HLA-DR/DQ, CD4) and are functionally active in primary mixed leucocyte reactions (MLR). The colony forming cells are the earliest progenitors of dendritic/Langerhans cells so far identified and have been termed dendritic Langerhans colony forming units - DL-CFU. This bone marrow culture technique has now been applied in order to characterise further these progenitors of dendritic cells and to determine their regulation by growth factors in-vitro.
KeywordsDendritic Cell Human Bone Marrow Human Tumour Necrosis Factor Early Progenitor Northwick Park Hospital
Unable to display preview. Download preview PDF.
- 2.Reid, C.D.L., A. Stackpoole, A. Meager, and J. Tikerpae. 1992. Interactions of tumour necrosis factor with granulocyte-macrophage colony-stimulating factor and other cytokines in the regulation of dendritic cell growth in vitro from early bipotent CD34+ progenitors in human bone marrow. J. Immunol. 149: 2681PubMedGoogle Scholar
- 3.Migliaccio, G., A.R. Migliaccio, and J. Adamson. 1988. In-vitro differentiation of human granulocyte/macrophage and erythroid progenitors: Comparitive analysis of the influence of recombinant human erythropoietin, G-CSF, GM-CSF, and IL-3 in serumsupplemented and serum deprived cultures. Blood 72: 248PubMedGoogle Scholar
- 4.Sonada, Y., Y.C. Yang, G.G. Wong, S.C. Clarke, and M. Ogawa. 1988. Analysis in serum free culture of the targets of recombinant human hemopoietic growth factors: Interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages. Proc. Natl. Acad. Sci. USA. 85: 4360CrossRefGoogle Scholar
- 5.Li Lu, K. Welte, J.L. Gabrilove, G. Hangoc, E. Bruno, R. Hoffman, and H.E. Broxmeyer. 1986. Effects of recombinant human tumour necrosis factor, recombinant human interferon, and prostaglandin E on colony formation of human hematopoietic progenitor cells stimulated by natural human pluripotent colony-stimulating factor, pluripoietin, and recombinant erythropoietin in serum-free cultures. Cancer Res. 46: 4357PubMedGoogle Scholar
- 6.Broxmeyer, H.E., D.E. Williams, Li Lu, S. Cooper, S.L. Anderson, G.S. Beyer, R. Hoffman, and B.Y. Rubin. 1986. The suppressive influences of human tumour necrosis factor on bone marrow hematopoietic progenitor cells from normal donors and patients with leukaemia: synergism of tumour necrosis factor and interferon gamma. J. Immunol. 136: 4487PubMedGoogle Scholar
- 8.Piacibello, W., F. Sanavio, A. Severino, S. Morelli, A.M. Vaira, A. Stacchini, and M. Aglietta. 1990. Opposite effect of tumour necrosis factor on granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor-dependent growth of normal and leukaemic hemopoietic progenitors. Cancer Res. 50: 5065PubMedGoogle Scholar
- 12.Koch, F., C. Heufler, E. Kampgen, D. Schneeweiss, G. Bock, and G. Schuler. 1990. Tumour necrosis factor maintains the viability of murine epidermal Langerhans cells in culture, but in contrast to granulocyte/macrophage colony-stimulating factor, without inducing their functional maturation. J. Exp. Med. 171: 159PubMedCrossRefGoogle Scholar