TNF and GM-CSF Dependent Growth of an Early Progenitor of Dendritic Langerhans Cells in Human Bone Marrow

  • Cecil D. L. Reid
  • Jaak Tikerpae
  • Arthur Stackpoole
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)


It has been demonstrated recently that colonies of cells with dendritic morphology can be generated in-vitro in semi-solid cultures of both human bone marrow and peripheral blood mononuclear cells1. The cells express antigens typically found on skin Langerhans cells (CD1a, HLA-DR/DQ, CD4) and are functionally active in primary mixed leucocyte reactions (MLR). The colony forming cells are the earliest progenitors of dendritic/Langerhans cells so far identified and have been termed dendritic Langerhans colony forming units - DL-CFU. This bone marrow culture technique has now been applied in order to characterise further these progenitors of dendritic cells and to determine their regulation by growth factors in-vitro.


Dendritic Cell Human Bone Marrow Human Tumour Necrosis Factor Early Progenitor Northwick Park Hospital 
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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Cecil D. L. Reid
    • 1
  • Jaak Tikerpae
    • 1
  • Arthur Stackpoole
    • 2
  1. 1.Department of HaematologyNorthwick Park Hospital and Clinical Research CentreHarrow, MiddlesexUK
  2. 2.Department of Transplantation BiologyNorthwick Park Hospital and Clinical Research CentreHarrow, MiddlesexUK

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