Differentiation of Dendritic Cells in Cultures of Rat and Mouse Bone Marrow Cells

  • William E. Bowers
  • Shengqiang Yu
  • Syeedur Khandkar
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)


Although dendritic cells originate from bone marrow, 1,2,3,4 they are not detectable in fresh preparations of bone marrow cells.5 We previously developed an in vitro system in which dendritic cells are produced when rat bone marrow cells are cultured for 4-5 days.6 We recently established an in vitro bone marrow culture system for mouse that enables us to use the greater number of recombinant cytokines, monoclonal antibodies, cell lines, and reagents available for this species. Similar results have been obtained in both species. This paper summarizes the major findings.


Dendritic Cell Bone Marrow Cell Bone Marrow Culture Mature Dendritic Cell Mouse Bone Marrow Cell 
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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • William E. Bowers
    • 1
  • Shengqiang Yu
    • 1
  • Syeedur Khandkar
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of South Carolina School of MedicineColumbiaUSA

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