Monocyte-Derived Langerhans Cells from Different Species - Morphological and Functional Characterization

  • Falko Steinbach
  • Bernhard Thiele
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)


It has been demonstrated earlier that peripheral blood monocytes can be differentiated into antigen presenting accessory cellse.g.1,2, and also that mouse blood contains precursors of dendritic cells3. In the human system we have recently demonstrated the in-vitro differentiation of a Langerhans cells phenotype from peripheral blood monocytes for the first time, 4 indicating the possibility to study the suggested pathway from monocytes via Langerhans cells (LC) to dendritic cells (DC)5 in-vitro.


Dendritic Cell Peripheral Blood Monocyte Accessory Cell Mixed Lymphocyte Reaction Autologous Serum 
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  1. 1.
    H.M. Najar, A.C. Bru-Capdeville, R.K.H. Gieseler and J.H. Peters, Differentiation of human monocytes into accessory cells at serum-free conditions, Eur. J.Cell Biol., 51: 339 (1990)PubMedGoogle Scholar
  2. 2.
    G. Rossi, N. Heveker, B. Thiele and H. Gelderblom, Mannan antigen presentation by monocyte-derived dendritic cells, in: Progress in AIDS research in the Fed. Rep. of Germany M. Schauzu, ed., MMV Medizin Verlag München (1990)Google Scholar
  3. 3.
    K. Inaba, R. Steinman, M. Witmer Pack, H. Aya, M. Inaba, T. Sudo, S. Wolpe and G. Schuler, Identification of proliferating dendritic cell precursors in mouse blood, J.Exp. Med., 175: 1157 (1992)PubMedCrossRefGoogle Scholar
  4. 4.
    G. Rossi, N. Heveker, B. Thiele, H. Gelderblom and F. Steinbach, Development of a Langerhans cell phenotype from peripheral blood monocytes, Imm. Lett. 32: 189 (1992)CrossRefGoogle Scholar
  5. 5.
    G. Schuler and R.M. Steinman, Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro, J. Exp. Med., 161: 526 (1985)PubMedCrossRefGoogle Scholar
  6. 6.
    F. Steinbach and B. Thiele, Phenotypical investigation of mononuclear phagocytes by FACS analysis, in: N.N., P. Poidron, D. Wachsman eds., Intercept, Andover, G.B.(in press)Google Scholar
  7. 7.
    M. Ormerod, Analysis of DNA, in: Flow Cytometry, M. Ormerod ed., IRL Press, Oxford (1990)Google Scholar
  8. 8.
    A.C Roche, P. Midoux, P. Bouchard and M. Monsigny, Membrane lectins on human monocytes, FEBS Lett., 193: 63 (1985)PubMedCrossRefGoogle Scholar
  9. 9.
    S. Miyauchi and K. Hashimoto, Mitotic activities of normal epidermal Langerhans cells, J.Invest.Dermatol., 92: 120 (1989)PubMedCrossRefGoogle Scholar
  10. 10.
    E.R. Flechner, P.S. Freudenthal, G. Kaplan and R.M. Steinman, Antigen-specific T-lymphocytes efficiently cluster with dendritic cells in the human primary mixed lymphocyte reaction, Cell. Imm., 111: 183 (1988)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Falko Steinbach
    • 1
  • Bernhard Thiele
    • 2
  1. 1.Dept. of VirologyFU BerlinBerlin 65Germany
  2. 2.Charité, Humboldt University of BerlinBerlinGermany

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