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The MHC Expression of Dendritic Cells from Mouse Spleen Isolated by Centrifugal Elutriation is Upregulated During Short Term Culture

  • Miriam A. Ossevoort
  • René E. M. Toes
  • Cornelis J. M. Melief
  • W. Martin Kast
  • Marloes L. H. De Bruijn
  • Carl G. Figdor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

Dendritic cells (DC) are extremely potent antigen presenting cells (APC), which can even induce an anti-viral specific primary T cell response in vitro (Macatonia et al, 1989). Both the quantitatively and qualitatively (fewer sialic acids) superior MHC expression and the large surface area of DC with long dendritic projections possibly allow a better interaction of the MHC-peptide complex on the DC with the T cell receptor (Boog et al., 1989). Generally, fewer DC than other types of APC are required to induce a specific T cell response (Kast et al., 1988).

Keywords

Dendritic Cell Sialic Acid Glass Petri Dish Short Term Culture Centrifugal Elutriation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Miriam A. Ossevoort
    • 1
  • René E. M. Toes
    • 1
  • Cornelis J. M. Melief
    • 1
  • W. Martin Kast
    • 1
  • Marloes L. H. De Bruijn
    • 2
  • Carl G. Figdor
    • 2
  1. 1.Department of Immunohematology and BloodbankUniversity HospitalRC, LeidenThe Netherlands
  2. 2.Division of ImmunologyThe Netherlands Cancer InstituteAmsterdamThe Netherlands

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