Abstract
Dendritic cells (DC) are extremely potent antigen presenting cells (APC), which can even induce an anti-viral specific primary T cell response in vitro (Macatonia et al, 1989). Both the quantitatively and qualitatively (fewer sialic acids) superior MHC expression and the large surface area of DC with long dendritic projections possibly allow a better interaction of the MHC-peptide complex on the DC with the T cell receptor (Boog et al., 1989). Generally, fewer DC than other types of APC are required to induce a specific T cell response (Kast et al., 1988).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Boog, C.J.P, Neefjes, J.J., Boes, J., Ploegh, H.L. and Melief, C.J.M., 1989, Specific immune responses restored by alteration in carbohydrate chains of surface molecules on antigen-presenting cells, Eur. J. Immunol. 19: 537.
Figdor, C.G., Van Es, W.L., Leemans, J.M.M. and Bont, W.S., 1984, A centrifugal elutriation system of separating small numbers of cells, J. Immunol. Meth. 68: 37.
Figdor, C.G., Te Velde, A.A., Leemans, J. and Bont, W.S., 1986, Differences in functional, phenotypical and physical properties of human peripheral blood monocytes(Mo) reflect their various maturation stages, in Leukocytes and Host Defense, J.J. Oppenheim and D.M. Jacobs, Alan R. Liss ed., New York, p. 283.
Girolomoni, G., Simon, J.C., Bergstresser, P.R. and Ponciano Jr, D.C., 1990, Freshly isolated spleen dendritic cells and epidermal langerhans cells undergo similar phenotypic and functional changes during short term culture, J. Immunol. 145: 2820
Kast, W.M., Boog, C.J.P., Roep, B.O., Voordouw, A.C. and Melief, C.J.M., 1988, Failure or succes in the restoration of virus-specific cytotoxic T lymphocyte response defects by dendritic cells, J. Immunol. 140: 3186.
Macatonia, S.E., Taylor, P.M., Knight, S.C. and Askonas, B.A., 1989, Primary stimulation by dendritic cells induces antiviral proliferative and cytotoxic T cell responses in vitro, J. Exp. Med. 169: 1255.
Nussenzweig, M.C., Steinman, R.M., Witmer, M.D. and Gutchinov, B., 1982, A monoclonal antibody specific for mouse dendritic cells, Proc. Natl. Acad. Sci. USA 79: 161.
Ossevoort, M.A., Toes, R.E.M., De Bruijn, M.L.H., Melief, C.J.M., Figdor, C.G. and Kast W.M., 1992, Rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation. J. Immunol. Meth. in press.
Pretlow, T.G. and Pretlow, T.P., 1979, Centrifugal elutriation (Counterstreaming centrifugation) of cells, Cell Biophysics, 1: 195.
Steinman, R.M., Kaplan, G., Witmer, M.D. and Cohn, Z., 1979, Identification of a novel cell-type in peripheral lymphoid organs of mice. V. Purification of spleen dendritic cells, new surface markers and maintenance in vitro, J. Exp. Med. 149: 1.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Plenum Press, New York
About this chapter
Cite this chapter
Ossevoort, M.A., Toes, R.E.M., Melief, C.J.M., Martin Kast, W., De Bruijn, M.L.H., Figdor, C.G. (1993). The MHC Expression of Dendritic Cells from Mouse Spleen Isolated by Centrifugal Elutriation is Upregulated During Short Term Culture. In: Kamperdijk, E.W.A., Nieuwenhuis, P., Hoefsmit, E.C.M. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 329. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-2930-9_31
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2930-9_31
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-6272-2
Online ISBN: 978-1-4615-2930-9
eBook Packages: Springer Book Archive