Morphological and Functional Differences Between HLA-DR+ Peripheral Blood Dendritic Cells and HLA-DR+ FN-Alpha Producing Cells
Several cell types, including B cells, NK cells, and monocytes have been proposed to be the cells responsible for IFN-α production in-vitro in response to herpesviruses and other viruses. When Perussia et al1 described IFN-α producing cells as HLA-DR+ nonadherent, non-monocyte, non-B peripheral blood cells, dendritic cells (DC) became an attractive candidate cells for this function. Subsequently, Bandyopadhyay et al2,3 showed that the major leukocyte subset in human peripheral blood responsible for IFN-α production upon culture with herpesvirus-infected target cells consists of nonadherent HLA-DR+ cells, which bear no surface markers of B, or T cells, monocytes or NK cells and that these cells also serve as accessory cells for NK cell-mediated lysis of the herpesvirus and HIV-infected targets. Later, Chehimi et al4 reported that human peripheral blood mononuclear cells contain at least two distinct HLA-DR+ cell subsets, both of which lack any B and T cell, monocyte and NK cell surface markers. One HLA-DR+ cell subset is plastic nonadherent, produces IFN-α in response to herpesvirus and HIV and provides accessory help to NK cells for lysis of virus-infected targets, but does not induce mixed leukocyte reaction (MLR). The other population has the morphology of dendritic cells (DC), is loosely adherent to plastic, stimulates T cells in the MLR, but neither produces IFN-α when exposed to virus-infected targets nor provides accessory help to NK cells. In this report, we show for the first time morphological, as well as new functional differences, between HLA-DR+ IFN-α producing cells (IPC) and HLA-DR+ DC.
KeywordsHuman Peripheral Blood Mononuclear Cell Accessory Cell Mixed Leukocyte Reaction Infected Target Cell Normal Peripheral Blood Mononuclear Cell
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