Abstract
Thymic dendritic cells (DC) are considered to be involved in negative selection of T cells leading to the acquisition of self-tolerance1,2, and many lines of evidence suggest that they may participate in the maturational process of thymocyte development3, in the clonal amplification of mature medullary thymocytes4, and in intrathymic presentation of non-MHC antigens5,6. Besides, recent reports analyzing the cell surface markers of murine thymic DC7,8 have demonstrated that these cells express some interesting molecules, particularly the CD8 antigen. The expression of CD8 by thymic DC may promote a mechanism for elimination of self-reactive thymocytes, as Sambhara and Miller9 have demonstrated that clonal deletion can be triggered if T cells that have been signaled through their TCR, also receive a second signal through the α3 domain of their class I MHC molecules, which can be delivered by a CD8 molecule.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
P. Matzinger and S. Guerder, Does T-cell tolerance require a dedicated antigen presenting cell?, Nature 338: 74 (1989).
F. Ramsdell and B.J. Fowlkes, Clonal deletion versus clonal anergy: the role of the thymus in inducing self tolerance, Science 248: 1342 (1990).
M. Lafontaine, D. Landry, N. Blanc-Brunât, M. Pelletier, and S. Montplaisir, IL-1 production by human thymic dendritic cells: studies on the interrelation with accessory function, Cell.Immunol. 135: 431 (1991).
D. Landry, L. Doyon, J. Poudrier, M. Lafontaine, M. Pelletier, and S. Montplaisir, Accessory function of human thymic dendritic cells in ConA induced proliferation of autologous thymocyte subsets, J. Immunol. 1990. 144: 836.
B. A. Kyewski, C. G. Fathman, and R. V. Rouse, Intrathymic presentation of circulating non-MHC antigens by medullary dendritic cells, J. Exp. Med. 163: 231 (1986).
M. Zöller, Intrathymic presentation by dendritic cells and macrophages: their role in selecting T cells with specificity for internal and external nominal antigen, Immunology 74: 407 (1991).
C. Ardavin and K. Shortman, Cell surface marker analysis of mouse thymic dendritic cells, Eur. J. Immunol 22: 859 (1992).
D. Vremec, M. Zorbas, R. Scollay, D. Saunders, C. Ardavin, L. Wu, and K. Shortman, The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells, J. Exp. Med. In press.
S. R. Sambhara and R. G. Miller, Programmed cell death of T cells signaled by the T cell receptor and the α3 domain of class I MHC, Science 252: 1424 (1991).
R. M. Steinman, The dendritic cell system and its role in immunogenicity, Annu. Rev. Immunol. 9: 271 (1991).
D. Landry, M. Lafontaine, M. Cossette, H. Barthelemy, C. Chartrand, S. Montplaisir, and Pelletier, Human thymic dendritic cells. Characterization, isolation and functional assays, Immunology 65: 135 (1988).
P. S. Freudenthal and R. M. Steinman, The distinct surface of human blood dendritic cells, as observed after an improved isolation method, Proc. Natl. Acad. Sci. USA 87: 7698 (1990).
M. Crowley, K. Inaba, M. Witmer-Pack, and R. M. Steinman, The cell surface of mouse dendritic cells: FACS analysis of dendritic cells from different tissues including thymus, Cell. Immunol. 118: 108 (1989).
A. M. Pollard and M. F. Lipscomb, Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells, J. Exp. Med. 172: 159 (1990).
G. C. MacPherson, Properties of lymph-borne (veiled) dendritic cells in culture I. modulation of phenotype, survival and function: partial dependence on GM-CSF, Immunology 68: 102 (1989).
G. A. van Seventer, Y. Shimizu, and S. Shaws, Roles of multiple accessory molecules in T-cell activation, Curr. Opin. Immunol 3: 294 (1991).
J. G. Damoiseaux, E. A. Dopp, J. J. Neefjes, R. H. Beelen, and C.D. Dijkstra, J. Leucocyte Biol. 46: 556 (1989).
A. Bellacosa and P. A. Lazo, The rat leukocyte antigen MRC OX-44 is a member of a new family of cell surface proteins which appear to be involved in growth regulation, Mol. Cell. Biol. 11: 2864 (1191).
D. Landry, M. Lafontaine, H. Barthelemy, N. Paquette, C. Chartrand, M. Pelletier, and S. Montplaisir, Human thymic dendritic cell-thymocyte association: ultrastructural cell phenotype analysis, Eur. J. Immnunol. 19: 1855 (1989).
M. T. Crowley, K. Inaba, M. D. Witmer-Pack, S. Gezelter, and R. M. Steinman, Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen, J. Immunol. Methods 133: 55 (1990).
G. G. MacPherson, S. Fossum, and B. Harrison, Properties of lymph-borne (veiled) dendritic cells in culture II. Expression of the IL-2 receptor: role of GM-CSF, Immunology 68: 108 (1989).
S. M. Setum, J. R. Serie, and O. D. Hegre, Comparative analysis of potency of splenic dendritic and adherent cell (macrophages) as alloantigen presenters in vivo, Diabetes 40: 1719 (1991).
R. Navarro, C. Ardavin, A. M. Fontecha, A. Alvarez, and A. Zapata, In vitro characterization of rat thymic macrophages, Immunology 73: 114 (1991).
C. L. Tucek and R. L. Boyd, Surface expression of CD4 and Thy-1 on mouse thymic stromal cells, Int. Immunol. 2: 593 (1990).
H. He, P. Naquet, D. Caillot, and M. J. Pierres, Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2+-independent mechanism, J. Exp. Med. 1991. 173: 515.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Plenum Press, New York
About this chapter
Cite this chapter
Purificacion Bañuls, M., Alvarez, A., Ferrero, I., Zapata, A., Ardavin, C. (1993). Rat Thymic Dendritic Cells: Flow Cytometry Analysis. In: Kamperdijk, E.W.A., Nieuwenhuis, P., Hoefsmit, E.C.M. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 329. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-2930-9_25
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2930-9_25
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-6272-2
Online ISBN: 978-1-4615-2930-9
eBook Packages: Springer Book Archive