Advertisement

Dendritic Cells Isolated From Rat and Human Non-Lymphoid Tissue are Very Potent Accessory Cells

  • Robert H. J. Beelen
  • Ellen van Vugt
  • Joke J. E. Steenbergen
  • Michiel G. H. Betjes
  • Carin E. G. Havenith
  • Eduard W. A. Kamperdijk
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

In the past it was shown that dendritic cells (DC) are the in vitro equivalent of interdigitating cells present in T-cell area’s of lymphoid organs (1). Recently it has been shown that DC can also be isolated from rat normal steady state peritoneal cells (PC) and that these numbers can be increased after intraperitoneal injection of BCG (2, 3). The same can be demonstrated from rat broncho-alveolar lavages after installation of BCG (4). Finally DC can be isolated from the PC population in human, especially from CAPD (continuous ambulant peritoneal dialysis) patients (5), which represent a chronic inflammatory state (6).

Keywords

Dendritic Cell Continuous Ambulant Peritoneal Dialysis Chronic Inflammatory State Peritoneal Cell Peritoneal Exudate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kamperdijk EWA et al, this symposium.Google Scholar
  2. 2.
    Vugt E van, Arkema JMS, Verdaasdonk MAM, Beelen RHJ, Kamperdijk EWA. Morphological and functional characteristics of rat steady state peritoneal dendritic cells. Immunobiol. 184, 14–24 (1991).CrossRefGoogle Scholar
  3. 3.
    Vugt E van, Verdaasdonk MAM, Beelen RHJ, Kamperdijk EWA. Induction of an increased number of dendritic cells in the peritoneal cavity of rats by i. p. administration of BCG. Immunobiol. in press (1992).Google Scholar
  4. 4.
    Havenith CEG, Breedijk AJ, Hoefsmit ECM. Effect of BCG inoculation on numbers of dendritic cells in bronchoalveolar lavages of rats. Immunobiol in press (1992).Google Scholar
  5. 5.
    Betjes MGH, Haks WC, Tuk CW, Beelen RHJ. Monoclonal antibody EBM 11 (CD 68) discriminates between dendritic cells and macrophages. Immunobiol. 183, 79–87 (1991).CrossRefGoogle Scholar
  6. 6.
    Bos HJ, Struijk D, Tuk CW, Veld JC de, Helmerhorst TJM, Hoefsmit ECM, Arisz L, Beelen RHJ. Letter to the editor: Peritoneal dialysis induces a local sterile inflammation and the mesothelial cells in the effluent are related to the bacterial peritonitis incidence. Nephron 59, 508–509 (1991).PubMedCrossRefGoogle Scholar
  7. 7.
    Beelen RHJ, Walker WS. Dynamics of cytochemically distinct subpopulations of macrophages in elicited peritoneal exudates. Cell. Immunol. 82: 246–257 (1983).PubMedCrossRefGoogle Scholar
  8. 8.
    Beelen RHJ, Eestermans IL, Dopp EA and Dijkstra CD. Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: expression of recognized antigens in different stages of differentiation. Transplant. Proc. 19: 3166–3170 (1987).Google Scholar
  9. 9.
    Vugt E van et al, this symposium.Google Scholar
  10. 10.
    Betjes MGH et al, this symposium.Google Scholar
  11. 11.
    Wijffels JFAM, Rover Z de, Rooyen N van, Kraal G, Beelen RHJ. Chronic inflammation induces the expression of dendritic cell markers not related to functional antigen presentation on peritoneal exudate macrophages. Immunobiol. 184, 83–92 (1991).CrossRefGoogle Scholar
  12. 12.
    Havenith CEG, Breedijk AJ, Verdaasdonk MAM, Kamperdijk EWA, Beelen RHJ. An improved and rapid method for the isolation of rat lymph or spleen T lymphocytes for T cell proliferation assays. J. Immunol. Methods, in press (1992).Google Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Robert H. J. Beelen
    • 1
  • Ellen van Vugt
    • 1
  • Joke J. E. Steenbergen
    • 1
  • Michiel G. H. Betjes
    • 1
  • Carin E. G. Havenith
    • 1
  • Eduard W. A. Kamperdijk
    • 1
  1. 1.Department of Cell Biology, Division of Electron MicroscopyFaculty Medicine, Free UniversityBT AmsterdamThe Netherlands

Personalised recommendations