Dendritic Cells Isolated From Rat and Human Non-Lymphoid Tissue are Very Potent Accessory Cells
In the past it was shown that dendritic cells (DC) are the in vitro equivalent of interdigitating cells present in T-cell area’s of lymphoid organs (1). Recently it has been shown that DC can also be isolated from rat normal steady state peritoneal cells (PC) and that these numbers can be increased after intraperitoneal injection of BCG (2, 3). The same can be demonstrated from rat broncho-alveolar lavages after installation of BCG (4). Finally DC can be isolated from the PC population in human, especially from CAPD (continuous ambulant peritoneal dialysis) patients (5), which represent a chronic inflammatory state (6).
KeywordsDendritic Cell Continuous Ambulant Peritoneal Dialysis Chronic Inflammatory State Peritoneal Cell Peritoneal Exudate
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- 1.Kamperdijk EWA et al, this symposium.Google Scholar
- 3.Vugt E van, Verdaasdonk MAM, Beelen RHJ, Kamperdijk EWA. Induction of an increased number of dendritic cells in the peritoneal cavity of rats by i. p. administration of BCG. Immunobiol. in press (1992).Google Scholar
- 4.Havenith CEG, Breedijk AJ, Hoefsmit ECM. Effect of BCG inoculation on numbers of dendritic cells in bronchoalveolar lavages of rats. Immunobiol in press (1992).Google Scholar
- 6.Bos HJ, Struijk D, Tuk CW, Veld JC de, Helmerhorst TJM, Hoefsmit ECM, Arisz L, Beelen RHJ. Letter to the editor: Peritoneal dialysis induces a local sterile inflammation and the mesothelial cells in the effluent are related to the bacterial peritonitis incidence. Nephron 59, 508–509 (1991).PubMedCrossRefGoogle Scholar
- 8.Beelen RHJ, Eestermans IL, Dopp EA and Dijkstra CD. Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: expression of recognized antigens in different stages of differentiation. Transplant. Proc. 19: 3166–3170 (1987).Google Scholar
- 9.Vugt E van et al, this symposium.Google Scholar
- 10.Betjes MGH et al, this symposium.Google Scholar
- 12.Havenith CEG, Breedijk AJ, Verdaasdonk MAM, Kamperdijk EWA, Beelen RHJ. An improved and rapid method for the isolation of rat lymph or spleen T lymphocytes for T cell proliferation assays. J. Immunol. Methods, in press (1992).Google Scholar