Phenotypical and Functional Characterization of Dendritic Cells in the Human Peritoneal Cavity

  • Michiel G. H. Betjes
  • Cees W. Tuk
  • Robert H. J. Beelen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)


Little is know about antigen presenting cells in the human peritoneal cavity. Mottolese et al. 1 studied the antigen presenting capacity of human peritoneal macrophages (the adherent cell fraction) and concluded that these cells could stimulate allogeneic and syngeneic T cells at a level similar to peripheral blood mononuclear cells. However it can not be excluded that contaminating dendritic cells (DC) biased these results.


Dendritic Cell Peritoneal Cell Continuous Ambulatory Peritoneal Dialysis Patient Antigen Present Capacity Blood Dendritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Mottolese M, P. G. Natali, G. Atlanta, A. Cavallari, F. Difilippo, and S. Ferrone. Antigenic profile and functional characterization of human peritoneal macrophages. J. Immunol. 135: 200 (1985).PubMedGoogle Scholar
  2. 2.
    Goldstein C. S., J. S. Bomalaski, R. B. Zurier, E. G. Neilson, and S. D. Douglas. Analysis of peritoneal macrophages in continuous ambulatory peritoneal dialysis patients. Kidney Int. 26: 733 (1984).PubMedCrossRefGoogle Scholar
  3. 3.
    Betjes M. G. H., C. W. Tuk, L. Arisz, and R. H. J. Beelen. Peritoneal Mϕ of patients treated with continuous ambulant peritoneal dialysis are very potent stimulator cells of an allogeneic mixed leukocyte reaction, in: Lymphatic Tissues and In-Vivo Immune Responses. Marcel Dekker, Inc., New York (1992).Google Scholar
  4. 4.
    Betjes M. G. H., M. C. Haks, C. W. Tuk, and R. H. J. Beelen. Monoclonal antibody EBM11 (anti-CD68) discriminates between dendritic cells and macrophages after short-term culture. Immunobiol. 183: 79 (1992).CrossRefGoogle Scholar
  5. 5.
    Van Voorhis, W. C., R. M. Steinman, L. S. Hair, J. Luban, M. D. Witmer, S. Koide, and Z. A. Cohn. Specific anti-mononuclear phagocyte monoclonal antibodies: application to the purification of dendritic cells and tissue localization of macrophages. J. Exp. Med. 158: 126 (1983).PubMedCrossRefGoogle Scholar
  6. 6.
    Barnstable C. J., W. F. Bodmer, G. Brown, G. Galfre, C. Milstein, A. F. Williams, and A. Ziegler. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens. Cell. 14: 9 (1978).PubMedCrossRefGoogle Scholar
  7. 7.
    Sanchez-Madrid, F., A. M. Krensky, C. F. Ware, E. Robbins, J. L. Strominger, S. J. Burakoff, and T. A. Springer. Three distinct antigens associated with human T-lymphocyte mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    Rothlein R., M. L. Dustin, S. D. Marlin, and T. A. Springer. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J. Immunol. 137: 1270 (1986).PubMedGoogle Scholar
  9. 9.
    Fithian, E., S. Kung, G. Goldstein, M. Rubenfeld, C. Fenoglio, R. Edelson. Reactivity of Langerhans cells with hybridoma antibody. Proc. Natl. Acad. Sci. USA. 78: 2541 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    Kuntz Crow M., and H. G. Kunkel. Human dendritic cells: major stimulators of the autologous and allogeneic mixed leucocyte reactions. Clin. Exp. Immunol. 49: 338 (1982).Google Scholar
  11. 11.
    Young J. W., and R. M. Steinman. Accesory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens as studied with a new technique for enriching blood dendritic cells. Cell. Immunol. 111: 167 (1988).PubMedCrossRefGoogle Scholar
  12. 12.
    Hart, D.N.J. and J. L. Mckenzie. Isolation and characterization of human tonsil dendritic cells. J. Exp. Med. 168: 157 (1988).PubMedCrossRefGoogle Scholar
  13. 13.
    Zvaifler N. J., R. M. Steinman, G. Kaplan, L. L. Lau, and M. Rivelis. Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J. Clin. Invest. 76: 789 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    Freudenthal P. S., R. M. Steinman. The distict surface of human blood dendritic cells, as observed after an improved isolation method. Proc. Natl. Acad. Sci. USA. 87: 7698 (1991).CrossRefGoogle Scholar
  15. 15.
    Toews, G. B., W. C. Vial, M. M. Dunn, P. Guzetta, G. Nunez, P. Stastny, and M. F. Lipscomb. The accessory cell function of human alveolar macrophages in specific T cell proliferation. J. Immunol. 132: 181 (1984).PubMedGoogle Scholar
  16. 16.
    Lipscomb, M. F., C. R. Lyons, G. Nunez, E. J. Ball, P. Stastny, W. Vial, V. Lem, J. Weisssler, L. M. Miller, and G. B. Toews. Human alvolar macrophages: HLA-DR-positive macrophages that are poor stimulators of a primary mixed leukocyte reaction. J. Immunol. 136: 497 (1986).PubMedGoogle Scholar
  17. 17.
    Havenith, C. E. G., A. J. Breedijk, and E. C. M. Hoefsmit. Effect of Bacillus Calmette-Guérin on numbers of dendritic cells in bronchoalveolar lavages of rats. Immunobiol. 184: 336 (1992).CrossRefGoogle Scholar
  18. 18.
    Bos H. J., D. G. Struijk, C. W. Tuk, J. C. de Veld, T. J. M Helmerhorst, E. C. M. Hoefsmit, L. Arisz, and R. H. J. Beelen. Peritoneal dialysis induces a local sterile inflammatory state and the mesothelial cells in effluent are related to the bacterial peritonitis incidence. Nephron. 59: 508 (1991).PubMedCrossRefGoogle Scholar
  19. 19.
    Scheeren, R. A., G. Koopman, S. van der Baan, C. J. L. M. Meijer, and S. T. Pals. Adhesion receptors involved in clustering of blood dendritic cells and T lymphocytes. Eur. J. Immunol. 21: 1101 (1991).PubMedCrossRefGoogle Scholar
  20. 20.
    Springer, T. A. Adhesion receptors of the immune system. Nature. 346: 425 (1990).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Michiel G. H. Betjes
    • 1
  • Cees W. Tuk
    • 1
  • Robert H. J. Beelen
    • 1
  1. 1.Department of Cell BiologyVrije UniversteitBT, AmsterdamThe Netherlands

Personalised recommendations