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The Influence of Dendritic Cells on T-Cell Cytokine Production

  • Erna van Wilsem
  • John Brevé
  • Georg Kraal
  • Ingrid van Hoogstraten
  • Huub Savelkoul
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

Pretreatment of mice with picryl chloride via the oral mucosa leads to a T-cell mediated tolerance after sensitization, while mice that do not receive this treatment react with a contact sensitivity (CS) response after topical skin application of picryl chloride(1). It is assumed that both in the oral epithelium and in the skin dendritic Langerhans cells are involved in the uptake of the antigen and subsequent transportation into the draining lymph nodes. Here the Langerhans cells will present antigen to T-cells in the para-cortical areas as interdigitating, dendritic cells.

Keywords

Dendritic Cell Oral Mucosa Drain Lymph Node ELISPOT Assay Oral Tolerance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    van Hoogstraten I. M. W., D. Boden, B. M. E. von Blomberg, G. Kraal, R. J. Scheper. Persistent immune tolerance to nickel and chromium by oral administration prior to cutaneous sensitization. J. Invest. Dermatol. in pressGoogle Scholar
  2. 2.
    Taguchi T., J. R. McGhee, R. L. Coffman, K. W. Beagley, J. H. Eldridge, K. Takatsu, and H. Kiyono. Detection of individual mouse splenic T-cells producing IFN-γ and IL-5 using the enzyme linked immunospot (ELISPOT) assay. J. Immunol. Methods 128: 65. (1990)PubMedCrossRefGoogle Scholar
  3. 3.
    Schumacher, J. H., A. O’Gara, B. Shrader, A. van Kimmenade, M. W. Bond, T. R. Mosmann, and R. L. Mosmann. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent assays. J. Immunol. 141: 1567. (1988)Google Scholar
  4. 4.
    Spitalny, G. L., and E. A. Havell. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560. (1984)PubMedCrossRefGoogle Scholar
  5. 5.
    Cherwinski, H. M., J. H. Schumacher, K. D. Brown, and T. R. Mosmann. Two types of mouse helper T-cell clone. III Further differences in lymphokine sythesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J. Exp. Med 166: 109. (1987)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Erna van Wilsem
    • 1
  • John Brevé
    • 1
  • Georg Kraal
    • 1
  • Ingrid van Hoogstraten
    • 2
  • Huub Savelkoul
    • 3
  1. 1.Dept. of Cell BiologyVrije UniversiteitAmsterdamThe Netherlands
  2. 2.Dept. of PathologyVrije UniversiteitAmsterdamThe Netherlands
  3. 3.Dept. of ImmunologyErasmus UniversityRotterdamThe Netherlands

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