Abstract
We sought to develop a T-dependent, clonal B cell microculture in order to assess changes in freguencies and Ig isotype potential of antigen (Ag)-specific B cells, associated with oral/gut mucosal exposure to Ag. Particularly, we were concerned with the development of IgA-memory B cells (1) as predictive of an in vivo, secondary mucosal IgA antibody (Ab) response (2) and with the role of germinal centers (GC) in Peyer’s patches (PP) in the generation of these IgA-memory cells as well as IgA pre-plasmablasts. Our original T/B microculture was based on clonal culturing of B cells responsive to thymus-independent Ags, as practiced in the Nossal laboratory (3) using Ag-specific B cells enriched by panning on haptenated gelatin (4), except that we used cloned, Ag (conalbumin)-specific D10, TH 2 cells (5) and haptenated-Ag as stimuli (6). This system exhibited H-2 haplotype restriction and a requirement for linked recognition of hapten and carrier. An Ag-independent version of this T/B microculture utilized the alloreactivity of the D1O cells versus I-Ab molecules and purified F1, k — b, B cells (6). Both systems promoted a high frequency of productive B cell clones (1-50%) that exhibited intraclonal isotype switching.
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References
D. A. Lebman, P. M. Griffin, and J. J. Cebra, Relationship between expression of IgA by Peyer’s patch cells and functional IgA memory cells, J. Exp. Med. 166: 1405 (1987).
J. A. Fuhrman and J. J. Cebra, Special features of the priming process for a secretory IgA response. B cell priming with Cholera toxin, J. Exp. Med. 153: 534 (1981).
G. J. V. Nossal, B. L. Pike, and F. L. Battye, Sequential use of hapten-gelatin fractionation and fluorescence activated cell sorting in the enrichment of haptenspecific B lymphocytes, Eur. J. Immunol. 8: 151 (1978).
P. A. Schweitzer and J. J. Cebra, Quality of antibodies secreted by clones in microcultures from B cells enriched on haptenated gelatin: isotypes and avidities, Mol. Immunol. 25: 231 (1988).
J. Kaye, S. Porcelli, J. Tite, B. Jones, and C. A. Janeway, Jr., Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836 (1983).
C. E. Schrader, A. George, R. L. Kerlin, and J. J. Cebra, Dendritic cells support production of IgA and other non-IgM isotypes in clonal microculture, Int. Immunol. 2: 563 (1990).
D. B. Kotloff and J. J. Cebra, Effect of TH-lines and clones on the growth and differentiation of B cells clones in microculture, Mol. Immunol. 25: 147 (1988).
R. M. Steinman, G. Kaplan, M. D. Witmer, and Z. A. Cohn, Identification of a novel cell type in peripheral lymphoid organs of mice. V. Purification of spleen dendritic cells, new surface markers, and maintenance in vitro. J. Exp. Med. 149: 1 (1979).
D. M. Spaulding, W. J. Koopman, J. H. Eldridge, J. R. McGhee, and R. M. Steinman, Accessory cells in murine Peyer’s patch. I. Identification and enrichment of a functional dendritic cell. J. Exp. Med. 157: 1646 (1983).
A. George and J. J. Cebra, Responses of single germinalcenter B cells in T-cell-dependent microculture, Proc. Natl. Acad. Sci. USA 88: 11 (1991).
R. L. Coffman, D. A. Lebman, and B. Shrader, Transforming growth factor-β specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes, J. Exp. Med. 170: 1039 (1989).
J. F. Elliott, Y. Lin, S. B. Mizel, R. C. Bleackley, D. G. Harnish, and V. Paetkau, Induction of Interleukin 2 messenger RNA inhibited by cyclosporin A, Science, 226: 1439 (1984).
C. B. Thompson, T. Lindsten, J. A. Ledbetter, S. L. Kunkel, H. A. Young, S. G. Emerson, J. M. Leiden, and C. H. June, CD28 activation pathway regulates the production of multiple T-cell-derived lymphokines/cytokines, Proc. Natl. Acad. Sci. USA 86: 1333 (1989).
A. Vallé, J-P. Aubry, I. Durand, and J. Banchereau, IL-4 and IL-2 upregulate the expression of antigen B7, the B cell counterstructure to T cell CD28: an amplification mechanism for T-B interactions, Int. Immunol. 3: 229 (1991).
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© 1993 Plenum Press, New York
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Schrader, C.E., Cebra, J.J. (1993). Dendritic Cell Dependent Expression of IgA By Clones in T/B Microcultures. In: Kamperdijk, E.W.A., Nieuwenhuis, P., Hoefsmit, E.C.M. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 329. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-2930-9_10
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DOI: https://doi.org/10.1007/978-1-4615-2930-9_10
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