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Physical Studies of Lipid Organization and Dynamics in Mycoplasma Membranes

  • Chapter
Mycoplasma Cell Membranes

Part of the book series: Subcellular Biochemistry ((SCBI,volume 20))

Abstract

The mycoplasmas are a diverse group of prokaryotic microorganisms that lack a cell wall. Since the mycoplasmas are genetically and morphologically the simplest organisms capable of autonomous replication, they provide useful models for the study of a number of problems in molecular and cellular biology. Mycoplasmas are particularly valuable for studies of the structure and function of cell membranes. Being nonphotosynthetic prokaryotes as well as lacking a cell wall or “outer membrane,” mycoplasma cells possess only a single membrane, the limiting or plasma membrane. This membrane contains essentially all the cellular lipid and, because these cells are small, a substantial fraction of the total cellular protein as well. Due to the absence of a cell wall, substantial quantities of highly purified membranes can usually be easily prepared by gentle osmotic lysis followed by differential centrifugation, a practical advantage not offered by other prokaryotic microorganisms. For a thorough discussion of the isolation and characterization of mycoplasma membranes, the reader is referred to a previous review by Razin (1979b).

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Authors and Affiliations

  1. Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7

    Ronald N. McElhaney

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  1. Ronald N. McElhaney
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  1. Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    Shlomo Rottem  & Itzhak Kahane  & 

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© 1993 Springer Science+Business Media New York

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McElhaney, R.N. (1993). Physical Studies of Lipid Organization and Dynamics in Mycoplasma Membranes. In: Rottem, S., Kahane, I. (eds) Mycoplasma Cell Membranes. Subcellular Biochemistry, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2924-8_3

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  • DOI: https://doi.org/10.1007/978-1-4615-2924-8_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6269-2

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