Abstract
Several families of negatively supercoiled topoisomers of the plasmid pIBI30 were 60Co γ-irradiated and assayed for the induction of strand scission by agarose gel electrophoresis. Form-I DNA for all topoisomers decreased exponentially with increasing dose. The radiation sensitivity (1/D37) was dependent on the average linking difference (ΔL) associated with a given supercoiled family. For ∣ΔL∣ < 2.5 the radiation sensitivity of DNA decreased with increasing ∣ΔL∣, whereas for ∣ΔL∣ > 2.5 enhanced radiation sensitivity was observed with increasing ∣ΔL∣. These results are in agreement with data reported by Miller, et al. (1991) also for pIBDO (250kV X-irradiated), but are inconsistent with experiments by Milligan, et al. (1992) for 137Cs γ-irradiated pUC18. Our results are suggestive of several mechanisms that could be operative in explaining the dependence of DNA radiation sensitivity on topology.
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Swenberg, C.E., Speicher, J.M., Miller, J.H. (1993). Does the Topology of Closed Supercoiled DNA Affect its Radiation Sensitivity?. In: Swenberg, C.E., Horneck, G., Stassinopoulos, E.G. (eds) Biological Effects and Physics of Solar and Galactic Cosmic Radiation. NATO ASI Series, vol 243A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2918-7_3
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DOI: https://doi.org/10.1007/978-1-4615-2918-7_3
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