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The First Thin Filament Layer Line Decreases in Intensity During an Isometric Contraction of Frog Skeletal Muscle

  • Katsuzo Wakabayashi
  • Hideaki Saito
  • Noriyoshi Moriwaki
  • Takakazu Kobayashi
  • Hidehiro Tanaka
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 332)

Abstract

During isometric contraction/activation of full-overlap and non-overlap live frog skeletal muscles, the intensity of the first thin filament layer line at the axial spacing of ∼1/37 nm-1, when separated from the partially overlapping first thick filament layer-line at ∼1/43 nm-1, remained unchanged in the inner radial region (0.02–0.08 nm-1) where a large intensity increase is observed in the rigor state. The intensity decreased in the outer radial region (0.08–0.18 nm-1) where this layer line is expected to peak in the resting state. The intensity decrease in the outer region became larger with increasing filament overlap; on activation of the non-overlap muscle, it was about half that of the full-overlap muscle. Thus the first thin filament layer line decreases in intensity and any indication of the rigor-like intensification is not observed at all during contraction. This intensity decrease can be attributed to the same structural changes giving rise to the intensity increase of the second thin filament layer line.

The results indicate that the configuration of the myosin heads interacting with actin during contraction differs significantly from that of the rigor state. Four-fold rotational symmetry of the thin filament structure as a whole becomes more pronounced during isometric contraction of the overlap muscle.

Keywords

Outer Region Isometric Contraction Thin Filament Myosin Head Layer Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Amemiya, Y., Wakabayashi, K., Tanaka, H., Ueno, Y. & Miyahara, J. Science 237, 164–168 (1987).PubMedCrossRefGoogle Scholar
  2. 2.
    Wakabayashi, K., Ueno, Y., Amemiya, Y. & Tanaka, H. Adv. Exp. Med. Biol. 226, 353–367 (1988).PubMedGoogle Scholar
  3. 3.
    Wakabayashi, K. & Amemiya, Y. in Handbook on Synchrotron Radiation Vol. 4 (eds. Ebashi, S., Koch, M. & Rubenstein, E.) 597–678 (North-Holland, Amsterdam, 1991).Google Scholar
  4. 4.
    Wakabayashi, K., Tanaka, H., Saito, H., Moriwaki, N., Ueno, Y. & Amemiya, Y. Adv. Biophys. 27, 3–13 (1991).PubMedCrossRefGoogle Scholar
  5. 5.
    Huxley, H.E. & Brown, W. J. Mol. Biol. 30, 383–434 (1967).PubMedCrossRefGoogle Scholar
  6. 6.
    Amemiya, Y., Wakabayashi, K., Hamanaka, T., Wakabayashi, T., Matsushita, T. & Hashizume, H. Nucl. Instrum. Methods 208, 471–477 (1983).CrossRefGoogle Scholar
  7. 7.
    Amemiya, Y. & Wakabayashi, K. Adv. Biophys. 27, 115–128 (1991).PubMedCrossRefGoogle Scholar
  8. 8.
    Yagi, N. Adv. Biophys. 27, 35–43 (1991).PubMedCrossRefGoogle Scholar
  9. 9.
    Yagi, N. & Matsubara, I. J. Mol. Biol. 208, 359–363 (1989).PubMedCrossRefGoogle Scholar
  10. 10.
    Huxley, H.E., Faruqi, A.R., Kress, M., Bordas, J. & Koch, M.HJ. J. Mol. Biol. 158, 637–684 (1982).PubMedCrossRefGoogle Scholar
  11. 11.
    Bordas, J., Diakun, G.P., Harries, J.E., Lewis, R.A., Mant, G.R., Martin-Fernandez, M.L. & Towns-Andrews, E. Adv. Biophys. 27, 15–33 (1991).PubMedCrossRefGoogle Scholar
  12. 12.
    Yagi, N. & Matsubara, I. Science 207, 307–308 (1980).PubMedCrossRefGoogle Scholar
  13. 13.
    Kress. M., Huxley, H.E., Faruqi, A.R. & Hendrix, J. J. Mol. Biol. 188, 325–342 (1986).PubMedCrossRefGoogle Scholar
  14. 14.
    Maeda, Y., Popp, D. & McLaughlin, S.M. Adv. Exp. Med. Biol. 226, 381–390 (1988).PubMedGoogle Scholar
  15. 15.
    Popp, D., Maeda, Y., Stewart, A.E.A. & Holmes, K.C. Adv. Biophys. 27, 89–103 (1991).PubMedCrossRefGoogle Scholar
  16. 16.
    Tsukita, S. & Yano, M. Nature 317, 182–184 (1985).PubMedCrossRefGoogle Scholar
  17. 17.
    Lenart, T.D., Allen, T.S., Barsotti, R.J., Ellis-Davies, G.C.R., Kaplan, J.H., Franzini-Armstrong, C. & Goldman, Y.E. This volume, 475-487.Google Scholar
  18. 18.
    Huxley, H.E., Kress, M., Faruqi, A.R. & Simmons, R.M. Adv. Exp. Med. Biol. 226, 347–352 (1988).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Katsuzo Wakabayashi
    • 1
  • Hideaki Saito
    • 1
  • Noriyoshi Moriwaki
    • 1
  • Takakazu Kobayashi
    • 2
  • Hidehiro Tanaka
    • 2
    • 3
  1. 1.Department Biophysical Engineering Faculty of Engineering ScienceOsaka UniversityToyonaka, OsakaJapan
  2. 2.Department of PhysiologySchool of Medicine Teikyo UniversityItabashi-ku, TokyoJapan
  3. 3.School of Nursing, Teikyo Heisei CollegeIchihara, ChibaJapan

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