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Mechanism of Myofilament Sliding in Muscle Contraction pp 393–407Cite as

Crossbridge Rotation in EDC-Crosslinked Striated Muscle Fibers

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 332))

Abstract

The rotatability of the strong- and weak-binding myosin heads was tested by stretching glycerinated rabbit psoas fibers after crosslinking the heads to actin by using a carbodiimide EDC. The equatorial 1,1 reflection intensity (I1,1) decreased by ∼10% upon 1% stretch in the presence of various ligands (ATP, ATP-γ-S, pyrophosphate and AMPPNP). As the action of ligands to dissociate actomyosin increased, the relaxation of tension response to stretch and the I1,1 decrease were accelerated. This result is best explained if the ligand converts the crosslinked head to a weak-binding state, in which the head is rotatable because of its acquired elasticity.

Conversely, the weak-to-strong transition was induced in the crosslinked system by removing a ligand (ATP-γ-S) from myosin. Force was produced upon weak-to-strong transition and was accounted for by the increased stiffness of each crosslinked myosin head. However, the comparison of stress-strain curves for the weak- and strong-binding myosin showed that the equilibrium angle of myosin attachment was unchanged, making it unlikely that the weak-to-strong transition is the sole mechanism for active contraction. The calcium-activated force of the same crosslinked fibers showed several features in marked contrast to the force produced by the weak-to-strong transition. This leads to a possibility that the active force is supported by a third class of intermediate which is distinct not only from the weak-binding but also from the strong-binding intermediates in a classical sense.

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Author information

Authors and Affiliations

  1. National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institutes of Health, Bethesda, Maryland, 20892, USA

    H. Iwamoto & R. J. Podolsky

  2. Department of Physiology, School of Medicine, Teikyo University, Itabashi-ku, Tokyo, 173, Japan

    H. Iwamoto

Authors
  1. H. Iwamoto
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  2. R. J. Podolsky
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Editor information

Editors and Affiliations

  1. Teikyo University, Tokyo, Japan

    Haruo Sugi

  2. University of Washington, Seattle, Washington, USA

    Gerald H. Pollack

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© 1993 Springer Science+Business Media New York

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Iwamoto, H., Podolsky, R.J. (1993). Crossbridge Rotation in EDC-Crosslinked Striated Muscle Fibers. In: Sugi, H., Pollack, G.H. (eds) Mechanism of Myofilament Sliding in Muscle Contraction. Advances in Experimental Medicine and Biology, vol 332. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2872-2_37

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  • DOI: https://doi.org/10.1007/978-1-4615-2872-2_37

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6245-6

  • Online ISBN: 978-1-4615-2872-2

  • eBook Packages: Springer Book Archive

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