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A New Crystal Form of Tropomyosin

  • Andrea Miegel
  • Lan Lee
  • Zbigniew Dauter
  • Yuichiro Maéda
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 332)

Abstract

Tropomyosin crystals with a new morphology have been obtained from lobster tail muscle tropomyosin from which 11 residues at the carboxyl-terminus have been proteolytically removed to avoid head-to-tail polymerization. In contrast to the conventional Bailey crystal form in which the elongated tropomyosin molecules form a mesh, in the present crystals the molecules are packed side-to-side with the long axes parallel to the c-axis of the crystal. The unit cell is tetragonal with a = b = 109 Å, c = 509 Å, and the symmetry is either P41212 or P43212, with 41(43) helical axes parallel to the c-axis. This suggests that a group of molecules surrounding a local 41 (43) axis is regarded as the building unit of the crystal. It is likely that the unit cell contains eight molecules with one molecule per asymmetric unit.

Keywords

Asymmetric Unit Screw Axis Rabbit Skeletal Muscle Fold Symmetry Axis Muscle Tropomyosin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Andrea Miegel
    • 1
  • Lan Lee
    • 1
  • Zbigniew Dauter
    • 1
  • Yuichiro Maéda
    • 1
  1. 1.European Molecular Biology Laboratory at DESYHamburg 52Germany

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