A New Crystal Form of Tropomyosin
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Tropomyosin crystals with a new morphology have been obtained from lobster tail muscle tropomyosin from which 11 residues at the carboxyl-terminus have been proteolytically removed to avoid head-to-tail polymerization. In contrast to the conventional Bailey crystal form in which the elongated tropomyosin molecules form a mesh, in the present crystals the molecules are packed side-to-side with the long axes parallel to the c-axis of the crystal. The unit cell is tetragonal with a = b = 109 Å, c = 509 Å, and the symmetry is either P41212 or P43212, with 41(43) helical axes parallel to the c-axis. This suggests that a group of molecules surrounding a local 41 (43) axis is regarded as the building unit of the crystal. It is likely that the unit cell contains eight molecules with one molecule per asymmetric unit.
KeywordsAsymmetric Unit Screw Axis Rabbit Skeletal Muscle Fold Symmetry Axis Muscle Tropomyosin
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