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Dependence of the Work Done by ATP-Induced Actin-Myosin Sliding on the Initial Baseline Force: Its Implications for Kinetic Properties of Myosin Heads in Muscle Contraction

  • H. Sugi
  • K. Oiwa
  • S. Chaen
Chapter
  • 108 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 332)

Abstract

The properties of the ATP-dependent actin-myosin sliding responsible for muscle contraction was studied using an in vitro force-movement assay system, in which a myosin-coated glass microneedle was made to slide on actin filament arrays (actin cables) in the giant algal cell with iontophoretic application of ATP. With a constant amount of ATP application, the amount of work done by the actin-myosin sliding increased with increasing baseline force from zero to 0.4–0.6 P 0, and then decreased with further increasing baseline force, thus giving a bell-shaped work versus baseline force relation. The result that the maximum actin-myosin sliding velocity did not change appreciably with increasing baseline force up to 0.4–0.6 P 0 implies, together with the limited number of myosin heads involved, that (1) the rate of power output of actin-myosin sliding is determined primarily by the amount of external load rather than the velocity of actin-myosin sliding, and (2) the bell shaped work versus baseline force relation (and also the hyperbolic force-velocity relation) results from the kinetic properties of individual myosin head rather than the change in the number of myosin heads involved.

Keywords

Myosin Head Load Relation Internodal Cell Actin Cable Iontophoretic Application 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • H. Sugi
    • 1
  • K. Oiwa
    • 1
  • S. Chaen
    • 1
  1. 1.Department of PhysiologySchool of Medicine Teikyo UniversityTokyoJapan

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