Actin pp 215-230 | Cite as

Control of p52(PAI-1) Gene Expression in Normal and Transformed Rat Kidney Cells: Relationship between p52(PAI-1) Induction and Actin Cytoarchitecture

  • Michael P. Ryan
  • Paul J. Higgins
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 358)


Alterations in cell shape or substrate adhesion often accompany changes in the expression and/or distribution of proteins that influence cellular architecture; these include structural elements which comprise the extracellular matrix (ECM), their transmembrane receptors (integrins) as well as components of the focal adhesion sites (focal contacts) (Spiegelman and Farmer, 1982; Ben-Ze’ev, 1986; Ben-Ze’ev, 1987; Dike and Farmer, 1988; Rodriguez et al., 1989; Dalton et al., 1992). Modulation of cell morphology and adhesivity may be a direct consequence of specific perturbations within the actin cytoskeleton. The actin-based microfilament network undergoes dramatic reorganization after exposure of cells to transforming retroviruses (Altenburg et al., 1976; Wang and Goldberg, 1976), growth factors (Bockus and Stiles, 1984; Herman and Pledger, 1985; Ridley and Hall, 1992) and microfilament-disrupting agents such as the cytochalasins (Goodman and Miranda, 1978; Schliwa, 1982; Cooper, 1987). Such induced architectural changes frequently signal specific changes in gene expression and cell growth behavior. In normal rat kidney (NRK) cells, for example, actin reorganization (associated with transformation by retroviral oncogenes or cell shape-modulating drugs) is typically reflected in morphologic restructuring, in reduced substrate adhesion and in the reprogramming of gene expression (Ryan and Higgins, 1988, 1989, 1991; Higgins and Ryan, 1989a, 1989b; Higgins et al., 1991). One gene which appears particularly susceptible to shape-associated expression in the NRK cell system encodes the 52-kDa type-1 inhibitor of plasminogen activator [p52(PAI-1)].


Focal Adhesion Kinase Actin Stress Fiber Focal Contact Substrate Adhesion Substrate Deposition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Michael P. Ryan
    • 1
  • Paul J. Higgins
    • 1
  1. 1.Department of Microbiology, Immunology and Molecular GeneticsAlbany Medical CollegeAlbanyUSA

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