Gelsolin Expression in Normal Human Keratinocytes is a Function of Induced Differentiation
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The epidermis is a self-renewing tissue comprised mainly of keratinocytes that exhibit different degrees of maturation depending upon their location (i.e. basal or suprabasal) within the tissue 1,2. Under non-perturbed conditions, the turnover rate within the epidermis is relatively slow and the keratinocytes exist primarily in a non-migratory mode with cells exhibiting attachment to the basement membrane and to other surrounding cells via families of surface receptors known as integrins and cadherins3,4. Upon partial or full thickness injury, keratinocytes become activated and assume a migratory phenotype5. This activation process includes modulation of integrin expression, actin reorganization and changes within the complement of actin-associated proteins that enable the keratinocyte to migrate over and through the provisional wound matrix to re-establish a complete epithelial barrier.
KeywordsTerminal Differentiation Human Epidermal Keratinocytes Basal Keratinocytes Normal Human Keratinocytes Cornified Envelope
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- 1.A.H. Mehregan, Normal structure of skin, in: “Pinkus’ Guide to Dermatohistopathology,” A.H. Mehregan, ed., Appleton-Century-Crofts, Norwalk CT (1986).Google Scholar
- 13.L. Staiano-Coico, M. Steinberg, and P.J. Higgins, Epidermal cell-shape regulation and subpopulation kinetics during butyrate-induced terminal maturation of normal and SV40-transformed human keratinocytes: Epithelial models of differentiation therapy, Int. J. Cancer 46: 733 (1990).PubMedCrossRefGoogle Scholar
- 27.R. Ford, G. Wang, P. Jannati, D. Adler, P. Racanelli, P.J. Higgins, and L. Staiano-Coico, Modulation of SPARC expression during butyrate-induced terminal differentiation of cultured human keratinocytes: Regulation via a TGF-b dependent pathway, Exp. Cell Res. 206: 261 (1993).PubMedCrossRefGoogle Scholar
- 28.G. Gabbiani, and G.B. Ryan, Development of a contractile apparatus in epithelial cells during epidermal and liver regeneration, J. Submicr. Cytol. 6: 143 (1974).Google Scholar