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Actin pp 159-168 | Cite as

Induction of Collagen Synthesis in Response to Adhesion and TGFβ is Dependent on the Actin-Containing Cytoskeleton

  • Jyotsna Dhawan
  • Stephen R. Farmer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 358)

Abstract

Adhesive interactions between cells and the extracellular matrix (ECM) are thought to influence gene expression. Previously, we showed that the regulation of α1(I) collagen expression is anchorage-dependent in Swiss 3T3 fibroblasts (Dhawan and Farmer, 1990). Non-adhesive culture conditions established by suspending cells in methylcellulose containing media result in a suppression of procollagen synthesis. Replating these suspended cells onto a tissue culture dish surface rapidly activates procollagen synthesis, and serum factors are not required for this response. The changes in type I procollagen synthesis reflect the levels of α1(I) collagen mRNA. Induction of procollagen synthesis during replating is the result of regulation at transcriptional and post-transcriptional sites (Dhawan et al., 1991). Collagen mRNAs are destabilized in suspended cells and restabilized when adhesive contacts are restored. Transcription of the α1(I) collagen gene is suppressed in suspended cells and reactivated by 18 hours after reattachment. In addition, the 5’ flanking region of the rat α1(I) collagen gene was found to contain sequences that conferred adhesion-responsiveness to a CAT reporter gene.

Keywords

Collagen Synthesis Collagen Gene Serum Response Factor Adhesive Interaction Collagen Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Jyotsna Dhawan
    • 1
  • Stephen R. Farmer
    • 1
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

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