Actin pp 159-168 | Cite as

Induction of Collagen Synthesis in Response to Adhesion and TGFβ is Dependent on the Actin-Containing Cytoskeleton

  • Jyotsna Dhawan
  • Stephen R. Farmer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 358)


Adhesive interactions between cells and the extracellular matrix (ECM) are thought to influence gene expression. Previously, we showed that the regulation of α1(I) collagen expression is anchorage-dependent in Swiss 3T3 fibroblasts (Dhawan and Farmer, 1990). Non-adhesive culture conditions established by suspending cells in methylcellulose containing media result in a suppression of procollagen synthesis. Replating these suspended cells onto a tissue culture dish surface rapidly activates procollagen synthesis, and serum factors are not required for this response. The changes in type I procollagen synthesis reflect the levels of α1(I) collagen mRNA. Induction of procollagen synthesis during replating is the result of regulation at transcriptional and post-transcriptional sites (Dhawan et al., 1991). Collagen mRNAs are destabilized in suspended cells and restabilized when adhesive contacts are restored. Transcription of the α1(I) collagen gene is suppressed in suspended cells and reactivated by 18 hours after reattachment. In addition, the 5’ flanking region of the rat α1(I) collagen gene was found to contain sequences that conferred adhesion-responsiveness to a CAT reporter gene.


Collagen Synthesis Collagen Gene Serum Response Factor Adhesive Interaction Collagen Expression 
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  1. Bellas, R.E., Bendori R., and Farmer, S.R., 1991, Epidermal growth factor activation of vinculin and β1-integrin gene transcription in quiescent Swiss 3T3 cells, J. Biol. Chem. 266: 12008.PubMedGoogle Scholar
  2. Ben-Ze’ev A., Farmer, S.R., and Penman, S., 1980, Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts, Cell 21: 365.CrossRefGoogle Scholar
  3. Dhawan J., and Farmer, S.R., 1990, Regulation of α1(I)-collagen gene expression in response to cell adhesion in Swiss 3T3 fibroblasts, J. Biol. Chem. 265: 9015.PubMedGoogle Scholar
  4. Dhawan J., Lichtler, A.C., Rowe, D.W., and Farmer, S.R., 1991, Cell adhesion regulates pro-α1(I) collagen mRNA stability and transcription in mouse fibroblasts, J. Biol. Chem. 266: 8470.PubMedGoogle Scholar
  5. Ignotz, R.A. and Massague, J., 1986, Transforming growth factor-β stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix, J. Biol. Chem. 261: 4337.PubMedGoogle Scholar
  6. Ignotz, R.A., Endo T., and Massague, J., 1987, Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-β. J. Biol. Chem. 262: 6443.PubMedGoogle Scholar
  7. Ignotz, R.A., and Massague, J., 1987, Cell adhesion protein receptors are targets for transforming growth factor-β, Cell 51: 189.PubMedCrossRefGoogle Scholar
  8. Kislauskis, E.H., and Singer, R.H., 1992, Determinants of mRNA localization. Current Opinions in Cell Biology, 4: 975.CrossRefGoogle Scholar
  9. Nugent, M.A. and Newman, M.J., 1989, Inhibition of normal rat kidney cell growth by transforming growth factor-β is mediated by collagen, J. Biol. Chem. 264: 18060.PubMedGoogle Scholar
  10. Ritzenthaler, J.D., Goldstein, R.H., Fine A., Lichtler A., Rowe, D.W., and Smith, B.D., 1991, Transforming growth factor-β activation elements in the distal promoter regions of the rat α1 type I collagen gene, Biochem. J. 280: 157.PubMedGoogle Scholar
  11. Ritzenthaler, J.D., Goldstein, R.H., Fine A., and Smith, B.D., 1993, Regulation of the α1 (I) collagen promoter via a transforming growth factor-β activation element, J. Biol Chem. 268: 13625.PubMedGoogle Scholar
  12. Rossi P., Karsenty G., Roberts, A.B., Roche, N.S., Sporn, M.B., and deCrombrugghe, B., 1988, A nuclear factor I binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-β, Cell 52: 405.PubMedCrossRefGoogle Scholar
  13. Svoboda, K.K.H., and Hay, E.D., 1987, Embryonic corneal epithelial interaction with exogenous laminin and basal lamina is F-actin dependent, Dev. Biol. 123: 455.PubMedCrossRefGoogle Scholar
  14. Sympson, G.J., and Geoghegan, T.E., 1990, Actin gene expression in murine erythroleukemia cells treated with cytochalasin D, Exp. Cell Res. 189: 28.PubMedCrossRefGoogle Scholar
  15. Tannenbaum J., and Brett, J.G., 1985, Evidence for regulation of actin synthesis in cytochalasin D treated HepG2 cells, Exp. Cell Res. 160: 435.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Jyotsna Dhawan
    • 1
  • Stephen R. Farmer
    • 1
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

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